Characterization of genetic diversity of puroindoline genes in Mexican wheat landraces
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Grain hardness is a major factor determining milling performance of common wheat. It determines the amount of damaged starch generated during milling, and therefore the end use of a given variety. One hundred and two lines from 15 Mexican wheat landraces were analyzed for grain hardness and for its genetic control. Sixteen lines were hard and 86 were soft-textured. All hard lines could be explained by a mutation in either the Pina-D1 or Pinb-D1 genes. In six hard lines there was no amplification of Pina-D1, suggesting that this gene was deleted (Pina-D1b allele). The remaining ten hard lines showed the presence of both Pina-D1 and Pinb-D1. Sequencing the Pinb-D1 genes of the hard lines revealed the presence of two different alleles (Pinb-D1b and Pinb-D1e). The results substantiate the importance of very old Mexican landraces as potential sources of genetic diversity for key quality traits in the development of modern wheat cultivars with different grain textures.
KeywordsCommon wheat Friabilin Genetic variability Grain texture
This research was supported by Grant AGL2010-19643-C02-01 from the Spanish Ministry of Science and Innovation, co-financed with European Regional Development Fund (FEDER) from the European Union. M. Ayala thanks to the Institutional Project Grant from the Fundación Carolina, Universidad de Córdoba (Spain) and Universidad Nacional de Asunción (Paraguay) for a fellowship to carry out a Masters degree. C. Guzmán thanks to the Spanish Ministry of Education (FPU program) for a predoctoral fellowship. The field help of Ana Moral is acknowledged.
- Edwards MA, Osborne BG, Henry RJ (2010) Puroindoline genotype, starch granule size distribution and milling quality of wheat. J Cereal Sci 52:314–320Google Scholar
- Greenwell P, Schofield JD (1986) A starch granule protein associated with endosperm softness in wheat. Cereal Chem 63:379–380Google Scholar
- Pomeranz Y, Williams PC (1990) Wheat hardness: its genetic, structural and biochemical background, measurement, and significance. Adv Cereal Sci Technol 10:471–546Google Scholar
- Stacey J, Isaac P (1994) Isolation of DNA from plants. In: Isaac PG (ed) Methods in molecular biology: protocols for nucleic acid analysis by non-radioactive probes. Humana Press, Totowa, pp 9–15Google Scholar
- Tanaka H, Morris CF, Haruna M, Tsujimoto H (2008) Prevalence of puroindoline alleles in wheat varieties from eastern Asia including the discovery of a new SNP in puroindoline b. Plant Genet Resour 6:142–152Google Scholar
- Tellez-Molina R, Alonso-Peña M (1952) Los trigos de la Ceres Hispanica de Lagasca y Clemente. Instituto Nacional de Investigaciones Agronómicas, MadridGoogle Scholar
- Velu G, Singh R, Huerta-Espino J, Peña J, Ortiz-Monasterio I (2011) Breeding for enhanced zinc and iron concentration in CIMMYT spring wheat germplasm. Czech J Genet Plant Breed 47:S174–S177Google Scholar
- Williams P, El-Haramein FJ, Nakkoul H, Rihawi S (1988) Crop quality evaluation methods and guidelines. Technical Manual 14 (Rev. 1). International Center for Agriculture Research in the Dry Areas, AleppoGoogle Scholar
- Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX (1997) Popgene version 1.3.2. The user-friendly software for population genetic analysis. Molecular Biology and Biotechnology Center, University of Alberta, AlbertaGoogle Scholar