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Euphytica

, Volume 186, Issue 1, pp 265–276 | Cite as

Genomic characterization of drought tolerance-related traits in spring wheat

  • Sundeep Kumar
  • Sunish Kumar Sehgal
  • Uttam Kumar
  • P. V. Vara Prasad
  • Arun Kumar Joshi
  • Bikram Singh Gill
Article

Abstract

Drought tolerance was investigated in ‘C306’, one of the most drought tolerant wheat cultivars bred in India in the 1960’s. An intervarietal mapping population of recombinant inbred lines of the cross ‘C306’ × ‘HUW206’ was evaluated for drought tolerance components, namely potential quantum efficiency of photosystem (PS) II (Fv/Fm), chlorophyll content (Chl), flag leaf temperature (Lt), and grain yield per plant (Gyp) under stress. Three independent experiments were conducted under well-watered and water-stressed conditions in greenhouses and growth chambers at Kansas State University (USA). Five hundred and sixty microsatellite markers covering the entire genome were screened for polymorphism between the parents. A QTL (QLt.ksu-1D) for Lt (low flag leaf temperature under stress) on the short arm of chromosome 1D between markers Xbarc271 and Xgwm337 at LOD 3.5 explained 37% of the phenotypic variation. A QTL for Fv/Fm (QF v /F m .ksu-3B) and Chl (QChl.ksu-3B) controlling quantum efficiency of PS II and chlorophyll content under stress were co-localized on chromosome 3B in the marker interval Xbarc68–Xbarc101 and explained 35–40% of the phenotypic variation for each trait. A QTL (QGyp.ksu-4A) for Gyp on chromosome 4A at a LOD value of 3.2 explained 16.3% of the phenotypic variation. Inconsistent QTLs were observed for Fv/Fm on chromosomes 3A, 6A, 2B, 4B, and 4D; for Chl on 3A, 6A, 2B and 4B; and for Lt on 1A, 3A 6A, 3B and 5B. The identified QTLs give a first glimpse of the genetics of drought tolerance in C306 and need to be validated in field experiments using the marker-phenotype linkages reported here.

Keywords

Chlorophyll content Chlorophyll fluorescence Leaf temperature Triticum aestivum QTL 

Notes

Acknowledgments

This research was supported by the Ministry of Science and Technology, Government of India, Delhi, through a Better Opportunities for Young Scientists in Chosen Areas of Science and Technology (BOYSCAST) Fellowship to Dr. Sundeep Kumar for doing advanced research at the Wheat Genetic and Genomic Resources Center, Kansas State University. We also acknowledge a Coordinated Agricultural Project Grant (no. 2011–68002–30029, Triticeae-CAP) from the USDA National Institute of Food and Agriculture for partial financial support. We thank Duane Wilson and Jon Raupp for excellent technical assistance. This is contribution no. 12-256-J from the Kansas Agricultural Experiment Station, Manhattan, KS 66506-5502.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Sundeep Kumar
    • 1
    • 2
  • Sunish Kumar Sehgal
    • 1
  • Uttam Kumar
    • 3
  • P. V. Vara Prasad
    • 4
  • Arun Kumar Joshi
    • 5
    • 6
  • Bikram Singh Gill
    • 1
    • 7
  1. 1.Wheat Genetic and Genomic Resources Center, Department of Plant PathologyKansas State UniversityManhattanUSA
  2. 2.NRC on DNA FingerprintingNational Bureau of Plant Genetic ResourcesNew DelhiIndia
  3. 3.The Energy and Resources Institute (TERI)New DelhiIndia
  4. 4.Department of AgronomyKansas State UniversityManhattanUSA
  5. 5.CIMMYT South AsiaKathmanduNepal
  6. 6.Genetics and Plant Breeding, Institute of Agricultural SciencesBanaras Hindu UniversityVaranasiIndia
  7. 7.Department of Biological Sciences, Faculty of Science, Genomics and Biotechnology SectionKing Abdulaziz UniversityJeddahSaudi Arabia

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