Euphytica

, Volume 186, Issue 2, pp 479–488 | Cite as

Induction and identification of hexadecaploid of Pinellia ternate

  • LiangYan He
  • Zhishan Ding
  • Fusheng Jiang
  • Bo Jin
  • Weiping Li
  • Xinghong Ding
  • Jinyue Sun
  • Guiyuan Lv
Article
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Abstract

Pinellia ternate (Thunb.) Breit (Araceae) is an important Chinese medicinal herb. In order to obtain the hexadecaploid plants of P. ternate with improved medicinal qualities, the shoot tips from in vitro-grown plants were treated with colchicine at various concentrations for different time to double the chromosome number. We obtained highest (42.5%) frequency of morphological variation when the tips were treated with 0.1% colchicine for 48 h. The putative hexadecaploid plants showed characteristics such as larger and thicker leaves, deeper green leaf color, stouter petiole, larger stomata, lower density of stomata across the lower epidermis of leaves and increased numbers of chloroplasts per stomata guard cell. Further analysis by flow cytometry and chromosome counting confirmed seven out of 129 were hexadecaploids. Besides, the growth rate (GR) of PLBs in hexadecaploid plants was 10.49% (increase rate in 1 day), 1.6 times higher than that of octoploid plants and the alkaloid content of PLBs in hexadecaploid plants was 0.0794%, 1.8 times higher than that of octoploid plant. The results indicated that we have established a successful procedure for development and propagation of hexadecaploid of P. ternate with higher GR and alkaloid content.

Keywords

Pinellia ternate Hexadecaploid Colchicine Flow cytometry Morphological characterization Alkaloid 

Abbreviations

PLBs

Protocorm-like bodies

ZJTCM

Zhejiang Chinese Medical University

6-BA

6-Benzylaminopurine

MS

Murashige and Skoog (1962) medium

NAA

1-Naphthaleneacetic acid

AC

Activated carbon

IBA

Indole-3-butyric acid

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Notes

Acknowledgments

The authors are grateful for the financial support of Zhejiang Key Innovation Team (Traditional Chinese Medicine Clinical Innovation Team) Research Fund Project (2009R50042), 2011 Zhejiang Provincial Science and Technology Agency Nonprofit Technology Research Project and 2010 Zhejiang Provincial University Students Science and Technology Innovation Program (Xinmiao Talents Scheme) (2010R410041).

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • LiangYan He
    • 1
  • Zhishan Ding
    • 1
  • Fusheng Jiang
    • 1
  • Bo Jin
    • 1
  • Weiping Li
    • 1
  • Xinghong Ding
    • 1
  • Jinyue Sun
    • 2
  • Guiyuan Lv
    • 1
  1. 1.Zhejiang Chinese Medical UniversityHangzhouPeople’s Republic of China
  2. 2.Plant Biotechnology InstituteNational Research Council of CanadaSaskatoonCanada

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