, Volume 186, Issue 3, pp 831–845 | Cite as

Variation in tolerance to radiant frost at reproductive stages in field pea germplasm

  • Shaista Shafiq
  • Diane E. Mather
  • Maqbool Ahmad
  • Jeffrey G. Paull


Radiant frost is a major abiotic stress, particularly at the reproductive stage, in field pea (Pisum sativum L.) grown in Mediterranean environments. Here, response to frost was studied for flowering stage (FS) organs (buds, flowers and set pods) and pod development stage (PDS) organs (flat, swollen and mature pods) under controlled conditions, with plants exposed to a minimum temperature of –4.8°C for 4 h. This frost treatment adversely affected seed yield through (i) abortion of buds, flowers and set pods (ii) death of pods and (iii) reduction in seed size. FS organs were more sensitive to frost than PDS organs. Genetic variation was observed among 83 accessions collected from 34 countries worldwide for survival of FS buds, flowers and set pods. In 60 of 83 accessions, no buds, flowers or set pods survived the frost treatment. Five accessions: ATC 104 (origin: United Kingdom), ATC 377 (Estonia), ATC 968 (Italy), ATC 3992 (Kazakhstan) and ATC 4204 (China), showed the highest frost tolerance of FS organs and lowest numbers of abnormal seeds. The frost tolerant accessions identified in this study may be useful as parents for breeding field pea varieties that will be less likely to suffer yield loss due to radiant frost during the reproductive stage.


Field pea Flowering stage Pod development stage Radiant frost Reproductive frost tolerance 


  1. Ahmad M, Shafiq S, Lake L (2010) Radiant frost tolerance in pulse crops—a review. Euphytica 172:1–12CrossRefGoogle Scholar
  2. Ahmed FE, Hall AE (1993) Heat injury during early floral bud development in cowpea. Crop Sci 33:764–767CrossRefGoogle Scholar
  3. Ali A, Johnson DL, Stushnoff C (1999) Screening lentil (Lens culinaris) for cold hardiness under controlled conditions. J Agric Sci 133:313–319CrossRefGoogle Scholar
  4. Aloni B, Karni L, Zaidman Z, Schaffer AA (1996) Changes of carbohydrates in pepper (Capsicum annuum L) flowers in relation to their abscission under different shading regimes. Ann Bot 78(2):163–168CrossRefGoogle Scholar
  5. Australian Bureau of Meteorology. Extremes of climate. Accessed September 2010
  6. Badaruddin M, Meyer DW (2001) Factors modifying frost tolerance of legume species. Crop Sci 41:1911–1916CrossRefGoogle Scholar
  7. Clarke HJ, Siddique KHM (2004) Response of chickpea genotypes to low temperature stress during reproductive development. Field Crops Res 90:323–334CrossRefGoogle Scholar
  8. Cousin R, Burghoffer A, Marget P, Vingere A, Eteve G (1993) Morphological, physiological and genetic basis of resistance in pea to cold and drought. In: Singh KB, Saxena MC (eds) Breeding for stress tolerance in cool-season food legumes. John Wiley and Sons, Sayce Publishing and ICARDA, West Sussex, pp 311–321Google Scholar
  9. GenStat Statistical Software 13th Edition. VSN international Ltd.
  10. Guilioni L, Wery J, Tardieu F (1997) Heat stress-induced abortion of buds and flowers in pea: is sensitivity linked to organ age or to relations between reproductive organs? Ann Bot 80:159–168CrossRefGoogle Scholar
  11. Knott CM (1987) A key for stages of development of the pea (Pisum sativum). Ann appl Biol 111:233–244CrossRefGoogle Scholar
  12. Knott CM, Belcher SJ (1998) Optimum sowing dates and plant populations for winter peas (Pisum sativum). J Agric Sci 131:449–454CrossRefGoogle Scholar
  13. Lejeune-Henaut I, Bourion V, Eteve G, Cunot E, Delhaye K, Desmyter C (1999) Floral initiation in field-grown forage peas is delayed to a greater extent by short photoperiods, than in other types of European varieties. Euphytica 109:201–211CrossRefGoogle Scholar
  14. Lejeune-Henaut I, Hanocq E, Bethencourt L, Fontaine V, Delbreil B, Morin J, Petit A, Devaux R, Boilleau M, Stempniak JJ, Thomas M, Laine AL, Foucher F, Baranger A, Burstin J, Rameau C, Giauffret C (2008) The flowering locus Hr colocalizes with a major QTL affecting winter frost tolerance in Pisum sativum L. Theor Appl Genet 116:1105–1116PubMedCrossRefGoogle Scholar
  15. Longvah T, Deosthale YG (1998) Nutrient composition and food potential of Parkia roxburghii, a less known tree legume from northeast India. Food Chem 62(4):477–481CrossRefGoogle Scholar
  16. McCullagh P, Nelder J (eds) (1989) Generalized linear models, 2nd edn. Chapman and Hall, CRC Press, UKGoogle Scholar
  17. Meyer DW, Badaruddin M (2001) Frost tolerance of ten seedling legume species at four growth stages. Crop Sci 41:1838–1842CrossRefGoogle Scholar
  18. Nayyar H (2005) Putrescine increases floral retention, pod set and seed yield in cold stressed chickpea. J Agron Crop Sci 191(5):340–345CrossRefGoogle Scholar
  19. Nayyar H, Bains T, Kumar S (2005) Low temperature induced floral abortion in chickpea: relationship to abscisic acid and cryoprotectants in reproductive organs. Environ Exp Bot 53(1):39–47CrossRefGoogle Scholar
  20. Nayyar H, Kaur G, Kumar S, Upadhyaya HD (2007) Low temperature effects during seed filling on chickpea genotypes (Cicer arietinum L.): probing mechanism affecting seed reserves and yield. J Agron Crop Sci 193:336–344CrossRefGoogle Scholar
  21. Nunes MC, Raymundo A, Sousa I (2006) Rheological behaviour and microstructure of pea protein/k-carrageenan/starch gels with different setting conditions. Food Hydrocolloids 20:106–113CrossRefGoogle Scholar
  22. Ohnishi S, Miyoshi T, Shirai S (2010) Low temperature stress at different flower developmental stages affects pollen development, pollination, and pod set in soybean. Environ Exp Bot 69(1):56–62CrossRefGoogle Scholar
  23. Poggio SL, Satorre EH, Dethiou S, Gonzalo GM (2005) Pod and seed numbers as a function of photothermal quotient during the seed set period of field pea (Pisum sativum) crops. Eur J Agron 22(1):55–69CrossRefGoogle Scholar
  24. Ridge PE, Pye DL (1985) The effects of temperature and frost at flowering on the yield of peas grown in a Mediterranean environment. Field Crops Res 12:339–346CrossRefGoogle Scholar
  25. Sasaki H, Ichimura K, Okada K, Oda M (1998) Freezing tolerance and soluble sugar contents affected by water stress during cold-acclimation and de-acclimation in cabbage seedlings. Sci Hortic 76(3–4):161–169CrossRefGoogle Scholar
  26. Siddique KHM, Loss SP, Regan KL, Jettner RL (1999) Adaptation and seed yield of cool season grain legumes in Mediterranean environments of south-western Australia. Aust J Agric Res 50:375–387CrossRefGoogle Scholar
  27. Srinivasan A, Johansen C, Saxena NP (1998) Cold tolerance during early reproductive growth of chickpea (Cicer arietinum L.): characterization of stress and genetic variation in pod set. Field Crops Res 57(2):181–193CrossRefGoogle Scholar
  28. Stoddard FL, Balko C, Erskine W, Khan HR, Link W, Sarker A (2006) Screening techniques and sources of resistance to abiotic stresses in cool-season food legumes. Euphytica 147:167–186CrossRefGoogle Scholar
  29. Tar’an B, Warkentin T, Somers DJ, Miranda D, Vandenberg A, Blade S, Bing D (2004) Identification of quantitative trait loci for grain yield, seed protein concentration and maturity in field pea (Pisum sativum L.). Euphytica 136:297–306CrossRefGoogle Scholar
  30. Tian S, Kyle WSA, Small DM (1999) Pilot scale isolation of proteins from field peas (Pisum sativum L.) for use as food ingredients. Int J Food Sci Tech 34:33–39CrossRefGoogle Scholar
  31. Woltz JM, Egli DB, TeKrony DM (2005) Freezing point temperatures of corn seed structures during seed development. Agron J 97(6):1564–1569CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Shaista Shafiq
    • 1
  • Diane E. Mather
    • 1
  • Maqbool Ahmad
    • 1
    • 2
  • Jeffrey G. Paull
    • 1
  1. 1.School of Agriculture, Food and Wine, Waite Research InstituteThe University of AdelaideGlen OsmondAustralia
  2. 2.South Australian Research and Development InstituteAdelaideAustralia

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