Confirmation of quantitative trait loci for resistance to multiple-HG types of soybean cyst nematode (Heterodera glycines Ichinohe)
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Genetic analysis of resistance of plant introduction (PI) 438489B to soybean cyst nematode (SCN) have shown that this PI is highly resistant to many SCN HG types. However, validation of the previously detected quantitative trait loci (QTL) has not been done. In this study, 250 F2:3 progeny of a Magellan (susceptible) × PI 438489B (resistant) cross were used for primary genetic mapping to detect putative QTL for resistance to five SCN HG types. QTL confirmation study was subsequently conducted using F6:7 recombinant inbred lines (RILs) derived from the same cross. Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers were employed for molecular genotyping. Interval mapping (IM), permutation tests, cofactor selection, and composite interval mapping (CIM) were performed to identify and map QTL. Results showed that five QTL intervals were associated with resistance to either multiple- or single-HG types of SCN. Among these, two major QTL for resistance to multiple-SCN HG types were mapped to chromosomes (Chr.) 8 and 18, consistent with the known rhg1 and Rhg4 locations. The other QTL were mapped to Chr. 4. The results of our study confirmed earlier reported SCN resistance QTL in this PI. Moreover, SSR and SNP molecular markers tightly linked to these QTL can be useful for the near-isogenic lines (NILs) development aiming to fine-mapping of these QTL regions and map-based cloning of SCN resistance candidate genes.
KeywordsSoybean (Glycine max) Soybean cyst nematode Quantitative trait loci Simple sequence repeat Single nucleotide polymorphisms
This research was supported by the United Soybean Board, Missouri Soybean Merchandizing Council, and National Center for Soybean Biotechnology (USDA-CSREES Award No. 2008-34555-19305). The authors acknowledge Dr. Md Pathan, John Wilcox, Md. Sariful Islam, Sabrina Brown, Kerry Clark, and Cuilan Liu, Division of Plant Sciences, University of Missouri, for their technical assistance. The authors would like to thank Drs. Perry Cregan and David Hyten, Soybean Genomics and Improvement Lab, USDA-ARS, Beltsville, MD 20705, for the development and early accessibility of a universal soybean single nucleotide polymorphism panel (USLP 1.0) used in this study.
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