Euphytica

, Volume 182, Issue 2, pp 157–166 | Cite as

Mapping a dominant negative mutation for triforine sensitivity in lettuce and its use as a selectable marker for detecting hybrids

  • Ivan Simko
  • Ryan J. Hayes
  • María José Truco
  • Richard W. Michelmore
Article

Abstract

Some lettuce cultivars are highly sensitive to triforine, an inhibitor of sterol biosynthesis found in some commercial systemic fungicides. First symptoms of a sensitive reaction are usually observed within 24–48 h after treatment and include severe wilting, necrosis and rapid plant death. We mapped a single dominant gene (Tr) that confers sensitivity of lettuce to triforine to linkage group 1 of the integrated genetic map of lettuce. The occurrence of sensitivity is not uniform across horticultural types of lettuce. While over 80% of green-romaine lettuce cultivars tested were sensitive, most cultivars of all other lettuce types were insensitive to triforine. All accessions of wild Lactuca spp. were insensitive to triforine. Allelism tests using F1 and F2 progeny revealed that sensitive cultivars of all horticultural types likely carry the same Tr gene. The dominant allele for sensitivity found in cultivated lettuce probably had a monophyletic origin. The reaction to triforine can be used as a marker for detecting hybrids originating from a cross between phenotypically similar parents with different responses to triforine treatment. It also provides an indication of genotypes for which applications of triforine-containing fungicides are inappropriate.

Keywords

Dominant mutation Lettuce Selectable marker Sterol biosynthesis inhibitor Triforine 

Abbreviations

cM

CentiMorgans

RILs

Recombinant-inbred lines

Tr gene

A dominant gene that confers sensitivity to triforine in lettuce

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Copyright information

© Springer Science+Business Media B.V. (outside the USA) 2011

Authors and Affiliations

  • Ivan Simko
    • 1
  • Ryan J. Hayes
    • 1
  • María José Truco
    • 2
  • Richard W. Michelmore
    • 2
  1. 1.Crop Improvement and Protection Research UnitUSDA-ARSSalinasUSA
  2. 2.The Genome Center and Department of Plant SciencesUniversity of CaliforniaDavisUSA

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