, Volume 179, Issue 3, pp 485–497

Quantitative trait loci analysis of stem strength and related traits in soybean



Stem strength is one of the major influencing factors of lodging in soybean [Glycine max (L.) Merr.] as well as other crops. To identify quantitative trait loci (QTL) associated with stem strength and related traits in soybean, a recombinant inbred line (RIL) population consisting of 165 lines derived from Zhongdou No. 29 × Zhongdou No. 32 was used in 3 years. Significant positive correlations were found among the four traits (stem strength, stem diameter, number of nodes, root dry weight). A linkage map spanning 1,240.7 cM was constructed using 245 SSR (simple sequence repeat) markers and a phenotypic marker (leaflet shape). By composite interval mapping and two-round strategy of QTL meta-analysis, 32 consensus QTL and 19 unique QTL were identified, respectively. Of eight pleiotropic unique QTL, two QTL (uq.A2-2 and uq.A2-3) located at the intervals of 23.2–26.8 and 38.5–42.4 cM on linkage group A2, respectively, were associated with all the four traits. Additive × environment (ae) interaction effects, epistasis (aa) and epistasis × environment (aae) interaction effects of QTL were detected as well. The results provide useful information for further genetic studies on stem strength of soybean.


Soybean Stem strength SSR QTL Meta-analysis 

Supplementary material

10681_2011_382_MOESM1_ESM.doc (42 kb)
Supplementary material 1 (DOC 41 kb)
10681_2011_382_MOESM2_ESM.doc (68 kb)
Supplementary material 2 (DOC 67 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Haifeng Chen
    • 1
  • Zhihui Shan
    • 1
  • Aihua Sha
    • 1
  • Baoduo Wu
    • 1
  • Zhonglu Yang
    • 1
  • Shuilian Chen
    • 1
  • Rong Zhou
    • 1
  • Xinan Zhou
    • 1
  1. 1.Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Oil Crop Biology of the Ministry of AgricultureWuhanChina

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