Abstract
The color of the seed coats of ‘Dorado’ (Phaseolus vulgaris L.) is garnet brown (dark red kidney bean color) and differs from most other dry bean varieties in the Honduran red bean market class. A genetic investigation of the color of ‘Dorado’ (same as DOR364) and G19833 (Liborino market class) seed coats was conducted. Crosses with genetic tester stocks demonstrated that the gene for garnet brown (GB) in ‘Dorado’ was not allelic with the R gene for dominant red (oxblood) seed coat. An allelism test between the ‘Dorado’ gene for GB seed coat and the rk drv gene for recessive expression of GB demonstrated allelism. We propose the gene symbol for Rk r for the ‘Dorado’ GB seed coat color gene. Rk r expresses partial dominance over Rk, where Rk r/Rk expresses a paler and highly variable intermediate red color. The interactions of Rk r, rk drv, and c u are discussed. Segregation analysis in the mapping population made up of DOR364 (same as ‘Dorado’) × G19833 recombinant inbred lines showed that the Rk r gene mapped to linkage group 1. The new allele at Rk was located at a distance of 17 cM from the RFLP marker Bng130 with a LOD > 3.0.
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References
Bassett MJ (1996) A complex C region genotype [? R] that with G B v lae produces dark seal-brown seedcoat color in common bean. J Am Soc Hort Sci 121:594–598
Bassett MJ (1998) A test cross protocol for determining the genotype of dark red seedcoat colors in common bean. J Am Soc Hort Sci 123:1048–1052
Bassett MJ (1999) The seedcoat color genotype of ‘Prim’ and the Manteca and Coscorrón market classes of common bean. HortScience 34:336–337
Bassett MJ (2004) A spontaneous mutation (rk drv) for expanded red flower veins at the rk d gene for dark red seed coat color. Annu Rep Bean Improv Coop 47:181–182
Bassett MJ (2007) Genetics of seed coat color and pattern in common bean. Plant Breed Rev 28:239–315
Bassett MJ, Miklas PN (2003) New alleles, rk cd and rk p, at the red kidney locus for seedcoat color in common bean. J Am Soc Hort Sci 128:552–558
Beebe SE, Rojas-Pierce M, Yan X, Blair MW, Pedraza F, Muñoz F, Tohme J, Lynch JP (2006) Quantitative trait loci for root architecture traits correlated with phosphorus acquisition in common bean. Crop Sci 46:413–426
Beninger CW, Hosfield GL, Bassett MJ, Owens S (2000) Classical and morphological expression of the B and Asp genes in Phaseolus vulgaris. J Am Soc Hort Sci 125:52–58
Blair MW, Pedraza F, Buendia HF, Gaitán-Solís E, Beebe SE, Gepts P, Tohme J (2003) Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.). Theor Appl Genet 107:1362–1374
Blair MW, Buendia HF, Giraldo MC, Metais I, Peltier D (2008) Characterization of AT-rich microsatellites in common bean (Phaseolus vulgaris L. Theor Appl Genet 118:91–103
Grisi MCM, Blair MW, Gepts P, Brondani C, Pereira PAA, Brondani RPV (2007) Genetic mapping of microsatellite markers in common bean (Phaseolus vulgaris) population BAT93 × Jalo EEP558. Genet Mol Res 6:691–706
Koinange EMK, Singh SP, Gepts P (1996) Genetic control of the domestication syndrome in common-bean. Crop Sci 36:1037–1045
Lamprecht H (1947) The seven alleles of the gene R of Phaseolus. Agric Hortic Genet 5:46–64
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg L (1987) Mapmaker an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Miklas PN, Delorme R, Stone V, Daly MJ, Stavely JR, Steadman JR, Bassett MJ, Beaver JS (2000) Bacterial, fungal, and viral disease resistance loci mapped in a recombinant inbred common bean population (‘Dorado’/XAN 176). J Am Soc Hortic Sci 125:476–481
Prakken R (1972) Inheritance of colours in Phaseolus vulgaris L. III On genes for red seedcoat colour and a general synthesis. Meded Landbouwhogeschool Wageningen 72-29:1–82
Smith FL (1939) A genetic analysis of red seed-coat color in Phaseolus vulgaris. Hilgardia 12:553–621
Smith FL (1947) Inheritance of seed-coat color in derivatives of Pinto beans. J Am Soc Agron 39:1039–1052
Smith FL, Madsen CB (1948) Seed-color inheritance in beans Interaction of the alleles at the R, Rk, and Bl loci in Phaseolus vulgaris. J Hered 39:191–194
Vallejos CE, Malandro JJ, Sheehy K, Zimmerman MJ (2000) Detection and cloning of expressed sequences linked to a target gene. Theor Appl Genet 101:1109–1113
Vallejos CE, Astua-Monge G, Jones V, Plyler TR, Sakiyama NS, MacKenzie SA (2006) Genetic and molecular characterization of the I locus of Phaseolus vulgaris. Genetics 172:1229–1242
Voysest OV (2000) Mejoramiento genético del frijol (Phaseolus vulgaris L.): Legado de variedades de América Latina 1930–1999. CIAT (International Center for Tropical Agriculture) Publication No. 321
Acknowledgments
We wish to thank Susan Duser of the Graphics Dept., Office of Academic Technology, University of Florida, for Fig. 1.
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CIAT prefers that mail be sent to them through a U.S. address rather than directly to the research station in Colombia. Centro Internacional de Agricultura Tropical (CIAT), Cali, Colombia (mail: 7343 NW 79th Terrace, Medley, Florida 33166).
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Bassett, M.J., Miklas, P.N., Caldas, G.V. et al. A dominant gene for garnet brown seed coats at the Rk locus in ‘Dorado’ common bean and mapping Rk to linkage group 1. Euphytica 176, 281–290 (2010). https://doi.org/10.1007/s10681-010-0247-3
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DOI: https://doi.org/10.1007/s10681-010-0247-3