, Volume 173, Issue 2, pp 265–277 | Cite as

A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers

  • Osman GulsenEmail author
  • Aydin Uzun
  • Ihsan Canan
  • Ubeyit Seday
  • Ercan Canihos


Sequence-related amplified polymorphism (SRAP), simple sequence repeats (SSR), inter-simple sequence repeat (ISSR), peroxidase gene polymorphism (POGP), resistant gene analog (RGA), randomly amplified polymorphic DNA (RAPD), and a morphological marker, Alternaria brown spot resistance gene of citrus named as Cabsr caused by (Alternaria alternata f. sp. Citri) were used to establish genetic linkage map of citrus using a population of 164 F1 individuals derived between ‘Clementine’ mandarin (Citrus reticulata Blanco ‘Clementine) and ‘Orlando’ tangelo’ (C. paradisi Macf. ‘Duncan’ × C. reticulata Blanco ‘Dancy’). A total of 609 markers, including 385 SRAP, 97 RAPD, 95 SSR, 18 ISSR, 12 POGP, and 2 RGA markers were used in linkage analysis. The ‘Clementine’ linkage map has 215 markers, comprising 144 testcross and 71 intercross markers placed in nine linkage groups. The ‘Clementine’ linkage map covered 858 cM with and average map distance of 3.5 cM between adjacent markers. The ‘Orlando’ linkage map has 189 markers, comprising 126 testcross and 61 intercross markers placed in nine linkage groups. The ‘Orlando’ linkage map covered 886 cM with an average map distance of 3.9 cM between adjacent markers. Segregation ratios for Cabsr were not significantly different from 1:1, suggesting that this trait is controlled by a single locus. This locus was placed in ‘Orlando’ linkage group 1. The new map has an improved distribution of markers along the linkage groups with fewer gaps. Combining different marker systems in linkage mapping studies may give better genome coverage due to their chromosomal target site differences, therefore fewer gaps in linkage groups.


Citrus reticulata Clementine Orlando tangelo F1 mapping Peroxidase Two-way pseudo-testcross strategy 



We thank Dr. Mikeal L. Roose for technical help and providing SSR primers. This study was funded by the Scientific and Technological Research Council (TUBITAK) with the project code TOVAG-105 O 628 and the General Directorate of Agricultural Research of Ministry of Agriculture and Rural Affairs, Turkey.

Supplementary material

10681_2010_146_MOESM1_ESM.doc (36 kb)
Supplementary material 1 (DOC 36 kb)


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Osman Gulsen
    • 1
    Email author
  • Aydin Uzun
    • 2
  • Ihsan Canan
    • 2
  • Ubeyit Seday
    • 2
  • Ercan Canihos
    • 3
  1. 1.Department of Horticulture, Faculty of AgricultureErciyes UniversityKayseriTurkey
  2. 2.Alata Horticultural Research InstituteMersinTurkey
  3. 3.Plant Protection Research InstituteAdanaTurkey

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