Euphytica

, Volume 168, Issue 1, pp 113–119 | Cite as

Over-expression of ArabidopsisFT gene reduces juvenile phase and induces early flowering in ornamental gentian plants

  • Takashi Nakatsuka
  • Yoshiko Abe
  • Yuko Kakizaki
  • Akiko Kubota
  • Norimoto Shimada
  • Masahiro Nishihara
Article

Abstract

Ornamental gentian plants are perennial and have a juvenile period of over 1 year before flowering. We transformed gentian plants with a construct comprising the ArabidopsisFLOWERING LOCUS T (FT) gene (encoding a major component protein of the flowering hormone ‘florigen’) under the control of the rolC promoter from Agrobacterium rhizogenes, which is known to induce vascular-specific expression. The resultant rolCpro-FT transgenic gentian plants showed early flowering in vitro and the earliest line formed floral buds within 4 months after transformation. Regeneration experiments from leaf explants of these rolCpro-FT transgenic plants also confirmed the early flowering phenotype. After acclimatization, these transgenic plants showed normal floral development in a closed greenhouse. There is no effective method to induce early flowering by cultivation management in gentian, therefore these lines might be very useful as annual early season cultivars.

Keywords

Early flowering FT Gentian In vitro flowering Transgenic plant 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Takashi Nakatsuka
    • 1
  • Yoshiko Abe
    • 1
  • Yuko Kakizaki
    • 1
  • Akiko Kubota
    • 1
  • Norimoto Shimada
    • 1
  • Masahiro Nishihara
    • 1
  1. 1.Iwate Biotechnology Research CenterKitakamiJapan

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