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Euphytica

, Volume 169, Issue 1, pp 1–17 | Cite as

Platanus × acerifolia genotypes surviving to inoculation with Ceratocystis platani (the agent of canker stain): first screening and molecular characterization

  • M. PilottiEmail author
  • A. Brunetti
  • L. Tizzani
  • O. Marani
Article

Abstract

Canker stain, caused by the fungus Ceratocystis platani, is a destructive disease in Platanus spp. It has been recently proved that resistant accessions can be produced and grown in Europe. However, additional resistant genotypes are still needed in order to avoid the onset of virulent pathogen strains favoured by the selection pressures exerted by genetically homogeneous resistant plane tree plantings. In this study we present the results of two parallel experiments performed on 975 accessions of P. × acerifolia seedlings and P. × acerifolia clones derived by cutting propagation from mature trees grown in the urban environments. The selection process was based on inoculation with C. platani and yielded 13 accessions that showed different types of resistant reactions and survived in a stable manner thoughout the period of observation. Selected accessions were characterised by sequencing the rDNA-ITS region and by developing PCR procedures capable of detecting P. orientalis and P. occidentalis LEAFY homoeologues. These molecular analyses enabled us to confirm the identification of the species, its hybrid origin and to assess an evident genetic variability among the accessions, which therefore have to be considered as different genotypes.

Keywords

Platanus Canker stain Ceratocystis platani Resistance ITS region LEAFY gene 

Notes

Acknowledgments

This ten-year long research could not have been realised without the vital support of pubblic Institutions and the help and engagement of many persons. The research project was funded over the years by Servizio Giardini—Comune di Roma. In particular we are grateful to Dr. Dario Esposito, Dr. Stefano Mastrangelo and Dr. Angelico Bonuccelli. Thanks also to Mr. Augusto Burini and Mr. Francesco Messina for their valuable contribution in supporting the research projects and to all the staff of Servizio Giardini that helped us in collecting and taking care of plant material. Molecular investigation was supported by MIPAF—Ministero delle Politiche Agricole e Forestali, project: “Proteine e geni per la protezione delle piante dagli stress biotici e abiotici, Proteo Stress—Genomica e Proteomica per lo studio della risposta di resistenza in specie arbore, ciliegio e platano”. We express our thanks to the coordinator of the project, Prof Felice Cervone (University La Sapienza, Rome). We would also like to mention Prof Paolo Capretti (University of Florence), Dr. Guido W. Grimm (Eberhard-Karls-University) and Dr. Angelo Porta-Puglia for their useful comments on the manuscript. Finally, we express our gratefulness to Dr. Andrè Vigouroux (INRA, France) whose research experience on the subject inspired us with the selection procedures in this study.

Supplementary material

10681_2009_9884_MOESM1_ESM.doc (130 kb)
Supplementary material (DOC 130 kb)

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • M. Pilotti
    • 1
    Email author
  • A. Brunetti
    • 1
  • L. Tizzani
    • 1
  • O. Marani
    • 1
  1. 1.Centro di Ricerca per la Patologia VegetaleConsiglio per la Ricerca e la Sperimentazione in Agricoltura, CRA-PAVRomeItaly

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