, Volume 173, Issue 3, pp 357–367 | Cite as

Inheritance mode of male sterility in bunching onion (Allium fistulosum L.) accessions

  • Ken-ichiro YamashitaEmail author
  • Hikaru Tsukazaki
  • Akio Kojima
  • Takayoshi Ohara
  • Tadayuki Wako


Cytoplasmic male sterility (CMS) is an indispensable trait for F1 hybrid seed production in bunching onion (Allium fistulosum L.). Expansion of the cytoplasmic diversity of F1 hybrid cultivars by introduction of various CMS resources has great potential to eliminate vulnerability to cytoplasm type-specific diseases. This study aimed to evaluate appearance frequency of male sterile plants in several bunching onion accessions and to identify CMS resources. In eight (‘Nogiwa Aigara’, ‘Bansei Hanegi’, ‘Amarume’, ‘Kimnung’, ‘Zhangqiu’, ‘INT/CHN/1990/GOTOU’, ‘Natsunegi’ and ‘Guangzhou’) of 135 accessions collected from Japan, China, Mongolia, Korea and Taiwan, male sterile plants appeared with varied frequencies from 1.7% (‘Nogiwa Aigara’ and ‘Bansei Hanegi’) to 24.5% (‘Zhangqiu’). The inheritance mode of Zhangqiu- and Guangzhou-derived male sterility was confirmed to be CMS by sib-crossings and interbreed crossings. Microscopic examination of microsporogenesis in the CMS plants revealed that microspore protoplasm rapidly degenerated without mitotic division after the release of microspores from tetrads. The CMS germplasm described here would be useful for the development of “A” lines to be used in F1 hybrid seed production of bunching onion. Male fertility in ‘Nogiwa Aigara’, ‘Bansei Hanegi’, ‘Kimnung’, ‘INT/CHN/1990/GOTOU’ and ‘Natsunegi’ was verified to be controlled by a single fertility restoration locus.


Allium fistulosum Bunching onion CMS Cytoplasm Male sterility 


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Ken-ichiro Yamashita
    • 1
    Email author
  • Hikaru Tsukazaki
    • 1
  • Akio Kojima
    • 1
  • Takayoshi Ohara
    • 1
  • Tadayuki Wako
    • 1
  1. 1.National Institute of Vegetable and Tea ScienceNational Agriculture and Food Research OrganizationTsuJapan

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