Euphytica

, 171:129

Genetic mapping within the wheat D genome reveals QTL for germination, seed vigour and longevity, and early seedling growth

Article

Abstract

Quantitative trait loci (QTL) controlling germination, seed vigour and longevity, and early seedling growth were identified using a set of common wheat lines carrying known D genome introgression segments. Seed germination (capacity, timing, rate and synchronicity) was characterized by a standard germination test, based either on the 1 mm root protrusion (germination sensu stricto) or the development of normal seedlings. To quantify seed vigour, the same traits were measured from batches of seed exposed for 72 h at 43°C and high (ca. 100%) humidity. Seed longevity was evaluated from the relative trait values. Seedling growth was assessed both under non-stressed and under osmotic stress conditions. Twenty QTL were mapped to chromosomes 1D, 2D, 4D, 5D, and 7D. Most of the QTL for germination sensu stricto clustered on chromosome 1DS in the region Xgwm1291Xgwm337. A region on chromosome 7DS associated with Xgwm1002 harboured loci controlling the development of normal seedlings. Seed vigour-related QTL were present in a region of chromosome 5DL linked to Xgwm960. QTL for seed longevity were coincident with those for germination or seed vigour on chromosomes 1D or 5D. QTL for seedling growth were identified on chromosomes 4D and 5D. A candidate homologues search suggested the putative functions of the genes within the respective regions. These results offer perspectives for the selection of favourable alleles to improve certain vigour traits in wheat, although the negative effects of the same chromosome regions on other traits may limit their practical use.

Keywords

Accelerated ageing Aegilops tauschii Plant genetic resources Triticum aestivum 

Abbreviations

AA

Accelerated ageing

CS

Chinese Spring

DILs

D genome introgression lines

IMA

Interval mapping analysis

ITMI

International Triticeae Mapping Initiative

LI

Longevity index

LOD

Logarithm of odds

PEG

Polyethylene glycol

SMA

Single marker analysis

QTL

Quantitative trait locus (loci)

SGT

Standard germination test

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Institute of GeneticsBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)GaterslebenGermany

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