Advertisement

Euphytica

, Volume 170, Issue 3, pp 355–364 | Cite as

Co-location of seed oil content, seed hull content and seed coat color QTL in three different environments in Brassica napus L.

  • X. Y. Yan
  • J. N. Li
  • F. Y. Fu
  • M. Y. Jin
  • L. Chen
  • L. Z. LiuEmail author
Article

Abstract

Increasing seed oil content is an important breeding goal for Brassica napus L. (B. napus). The identification of quantitative trait loci (QTL) for seed oil content and related traits is important for efficient selection of B. napus cultivars with high seed oil content. To get better knowledge on these traits, a molecular marker linkage map for B. napus was constructed with a recombinant inbred lines (RIL) population. The length of the map was 1,589 cM with 451 markers distributed over 25 linkage groups. QTL for seed oil content, seed hull content and seed coat color in three environments were detected by composite interval mapping (CIM) tests. Eleven QTL accounted for 5.19–13.57% of the variation for seed oil content. Twelve QTL associated with seed hull content were identified with contribution ranging from 5.80 to 22.71% and four QTL for seed coat color accounted for 5.23–15.99% of the variation. It is very interesting to found that co-localization between QTL for the three traits were found on N8. These results indicated the possibility to combine favorable alleles at different QTL to increase seed oil content, as well as to combine information about the relationship between seed oil content and other traits.

Keywords

Brassica napus L. Quantitative trait loci (QTL) Seed oil content Seed hull content Seed coat color 

Abbreviations

QTL

Quantitative trait loci

RIL

Recombinant inbred lines

SRAP

Sequence related amplified polymorphism

SSR

Simple sequence repeat

AFLP

Amplified fragment length polymorphism

TRAP

Target region amplified polymorphism

cM

Centimorgans

CIM

Composite interval mapping

PCR

Polymerase chain reaction

DNA

Deoxyribonucleic acid

CTAB

Cetyl trimethyl ammonium bromide

LOD

Log likelihood ratio

OC

Seed oil content

HC

Seed hull content

SC

Seed coat color

Notes

Acknowledgments

Financial support from the State “973” Project (2006CB101604) of China and a grant (30330400) from the Natural Science Foundation of China.

References

  1. Badani AG, Wittkop B, Lühs W, Baetzel R, Horn R, De Haro A, Font R, Friedt W, Snowdon RJ (2006) Colocalization of a partially dominant gene for yellow seed color with a major QTL influencing acid detergent fiber (ADF) content in different crosses of oilseed rape (Brassica napus). Genome 49:1499–1509CrossRefPubMedGoogle Scholar
  2. Basten CJ, Weir BS, Zeng ZB (1999) QTL cartographer, Version 2.5: programme in statistical genetics. Raleigh, North Carolina State UniversityGoogle Scholar
  3. Burns MJ, Barnes SR, Bowman JG, Clarke MHE, Werner CP, Kearsey MJ (2003) QTL analysis of an intervarietal set of substitution lines in Brassica napus: (i) seed oil content and fatty acid composition. Heredity 90:39–48CrossRefPubMedGoogle Scholar
  4. Cao G, Zhu J, He C, Gao Y, Yan J, Wu P (2001) Impact of epistasis and QTL and environment interaction on the developmental behavior of plant height in rice (Oryza sativa L.). Theor Appl Genet 103:153–160CrossRefGoogle Scholar
  5. Cheung WY, Landry BS (1998) Molecular mapping of seed quality traits in Brassica juncea L. Czern. and Coss. Acta Hortic 459:139–147Google Scholar
  6. Daun JK, DeClercq DR (1988) Quality of yellow and dark seeds in Brassica campestris canola varieties Candle and Tobin. J Am Oil Chem Soc 65:122–126CrossRefGoogle Scholar
  7. Delourme R, Falentin C, Huteau V, Clouet V, Horvais R, Gandon B, Specel S, Hanneton L, Dheu JE, Deschamps M, Margale E, Vincourt P, Renard M (2006) Genetic control of oil content in oilseed rape (Brassica napus L.). Theor Appl Genet 113:1331–1345CrossRefPubMedGoogle Scholar
  8. Ecke W, Uzunova M, Weißleder K (1995) Mapping the genome of rapeseed (Brassica napus L.). II. Localization of genes controlling erucic acid synthesis and seed oil content. Theor Appl Genet 91:972–977CrossRefGoogle Scholar
  9. Engqvist GM, Becker HC (1991) Relative importance of genetic parameters for selecting between oilseed rape crosses. Hereditas 115:25–30CrossRefGoogle Scholar
  10. Ferriol M, Pico B, Nuez F (2003) Genetic diversity of some accessions of Cucurbita maxima from Spain using RAPD and SRAP markers. Genet Resour Crop Evol 50:227–238CrossRefGoogle Scholar
  11. Fu FY, Liu LZ, Chai YR, Chen L, Yang T, Jin MY, Ma AF, Yan XY, Zhang ZS, Li JN (2007) Localization of QTLs for seed color using recombinant inbred lines of Brassica napus in different environments. Genome 50:840–854CrossRefPubMedGoogle Scholar
  12. Grami B, Stefansson BR (1977) Gene action for protein and oil content in summer rape. Can J Plant Sci 57:625–631Google Scholar
  13. Gül MK (2002) QTL-Kartierung und Analyse von QTL × Stickstoff Interaktionen beim Winterraps (Brassica napus L.). Ph.D. thesis, Uni Göttingen, Germany, pp 34–38Google Scholar
  14. Hu JG, Vick BA (2003) Target region amplification polymorphism: a novel marker technique for plant genotyping. Plant Mol Biol Rep 21:289–294CrossRefGoogle Scholar
  15. Lacape JM, Nguyen TB, Thibivilliers S, Bojinov B, Courtois B, Cantrell RB, Burr B, Hau B (2003) A combined RFLP-SSR-AFLP map of tetraploid cotton based on a Gossypium hirsutum × Gossypium barbadense backcross population. Genome 46(4):612–626CrossRefPubMedGoogle Scholar
  16. Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103:455–461CrossRefGoogle Scholar
  17. Li JN, Chen L, Tang ZL, Zhang XK, Yan SJ (2001) Genetic study and commercial application of the yellow-seeded rapeseed (Brassica napus L.). In: Proceedings of the international symposium on rapeseed science, Science Press, New York, Date Month Year, pp 28–34Google Scholar
  18. Liu LZ, Meng JL, Lin N, Chen L, Tang ZL, Zang XK, Li JN (2006) QTL mapping of seed coat color for yellow seeded Brassica napus. Acta Genetica Sinica 33:181–187CrossRefPubMedGoogle Scholar
  19. Lombard V, Delourme R (2001) A consensus linkage map for rapeseed (Brassica napus L.): construction and integration of three individual maps from DH populations. Theor Appl Genet 103:491–507CrossRefGoogle Scholar
  20. McCouch SR, Cho YG, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T (1997) Report on QTL nomenclature. Rice Genet Newslett 14:11–13Google Scholar
  21. Murry HG, Thomspon WF (1980) Rapid isolation of high molecular weight DNA. Nucleic Acids Res 8:4321–4322CrossRefGoogle Scholar
  22. Ochodzki P, Piotrowska A, Sorensen H (2003) Physical properties and chemical composition of whole seeds, hulls and meals of yellow-seeded rape grown in Poland. 1lth International Rape seed Congress, Copenhagen, Denmark, July, 21Google Scholar
  23. Parkin IAP, Sharpe AG, Lydiate DJ (2003) Patterns of genome duplication within the Brassica napus genome. Genome 46:291–303CrossRefPubMedGoogle Scholar
  24. Piepho HP (2000) A mixed-model approach to mapping quantitative trait loci in barley on the basis of multiple environment data. Genetics 156:2043–2050PubMedGoogle Scholar
  25. Piquemal J, Cinquin E, Couton F, Rondeau C, Seignoret E, Doucet I, Perret D, Villeger MJ, Vincourt P, Blanchard P (2005) Construction of an oilseed rape (Brassica napus L.) genetic map with SSR markers. Theor Appl Genet 111:1514–1523CrossRefPubMedGoogle Scholar
  26. Qiu D, Morgan C, Shi J, Long Y, Liu J, Li R, Zhuang X, Wang Y, Tan X, Dietrich E, Weihmann T, Everett C, Vanstraelen S, Beckett P, Fraser F, Trick M, Barnes S, Wilmer J, Schmidt R, Li J, Li D, Meng J, Bancroft I (2006) A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content. Theor Appl Genet 114:67–80CrossRefPubMedGoogle Scholar
  27. Rahman MH, Joersbo M, Poulson MH (2001) Development of yellow-seeded Brassica napus of double low quality. Plant Breed 120:473–478CrossRefGoogle Scholar
  28. SAS Institute (1989) SAS/STAT users guide: version 6, vol. 2, fourthed. SAS Institute Inc. Cary, North Carolina, USAGoogle Scholar
  29. Shen JX, Fu TD, Yang GS, Ma CZ, Tu JX (2005) Genetic analysis of rapeseed self-incompatibility lines reveals significant heterosis of different patterns for yield and oil content traits. Plant Breed 124:111–116CrossRefGoogle Scholar
  30. Stam PV, Ooijen JW (1995) Join Map(tm) version 3.0: software for the calculation of genetic linkage map, CPRO-DLO Wagemingen, The Netherlands, pp 1–5Google Scholar
  31. Stringam GR, McGregor DI, Pawlowski SH (1974) Chemical and morphological characteristics associated with seed coat color in rapeseed. In: Wratten N, Salisbury PA (eds) Proceedings of the 4th International Rapeseed Conference, The Regional Institute, Gosford, Australia, pp 99–108Google Scholar
  32. Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 1:77–78CrossRefGoogle Scholar
  33. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeay M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414CrossRefPubMedGoogle Scholar
  34. Wang R, Li JN, Chen L, Tang ZL, Zhang XK (2003) Genetic correlation analysis for main characters in yellow-seeded rapeseed lines (Brassica napus L.). Chin J Oil Crop Sci 25:8–11Google Scholar
  35. Zhao J (2002) QTLs for oil content and their relationships to other agronomic traits in an European × Chinese oilseed rape population. PhD thesis, faculty of agricultural sciences, George-August Uni Göttingen, GermanyGoogle Scholar
  36. Zhao J, Becker HC, Zhang D, Zhang Y, Ecke W (2005) Oil content in an European × Chinese rapeseed population: QTL with additive and epistatic effects and their genotype-environment interactions. Crop Sci 45:51–59CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • X. Y. Yan
    • 1
  • J. N. Li
    • 1
  • F. Y. Fu
    • 1
  • M. Y. Jin
    • 1
  • L. Chen
    • 1
  • L. Z. Liu
    • 1
    Email author
  1. 1.Chongqing Engineering Research Center for RapeseedCollege of Agronomy and Biotechnology, Southwest UniversityBeibei, ChongqingPeople’s Republic of China

Personalised recommendations