Euphytica

, 171:111 | Cite as

Mapping a diploid wheat abscisic acid 8′-hydroxylase homologue in the seed dormancy QTL region on chromosome 5Am

  • Shingo Nakamura
  • Makiko Chono
  • Fumitaka Abe
  • Hideho Miura
Article
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Abstract

In Arabidopsis, two genes of abscisic acid (ABA) 8′-hydroxylase (cytochrome P450 (CYP) 707A1 and A2) have been found to play important roles in seed dormancy through the regulation of ABA content in seeds. In order to examine the role of wheat ABA 8′-hydroxylase gene in seed dormancy, a diploid wheat ABA 8′-hydroxylase gene was cloned that showed high similarity to a barley ABA8′-hydroxylase gene (HvABA8′OH-2), and the cloned gene was designated as TmABA8′OH-2. Using recombinant inbred lines derived from a cross between diploid wheat Triticum boeoticum L. (Tb) and Triticum monococcum L. (Tm), TmABA8′OH-2 has been mapped to near the centromeric region of the long arm of chromosome 5Am, where the major seed dormancy QTL has been previously found. Comparison of the deduced amino acid sequences of TmABA8′OH-2 between Tb and Tm revealed five amino acid residue substitutions. These amino acid residues have distinctly different characteristics, and one of the substitutions occurs in the highly conserved amino acid residues in CYP707A family, indicating that these substitutions may have effects on the enzyme activities. Moreover, hexaploid wheat TmABA8′OH-2 homologue revealed that the level of its expression during seed development peaks at mid-maturation stage. This resembles the expression pattern of the Arabidopsis CYP707A1, which was shown to control seed dormancy. These results imply a possibility that TmABA8′OH-2 might be involved in seed dormancy, and associated with the QTL on chromosome 5Am.

Keywords

Wheat Dormancy SD1 locus Abscisic acid Abscisic acid 8′ hydroxylase 
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Notes

Acknowledgments

We thank former Professor T. Sasakuma of the Kihara Institute of Yokohama City University and Professor K. Noda, Okayama University for providing diploid wheat RILs for mapping, and H. Morishige at National Institute of Crop Science for her technical assistance. This research was supported by a “Development of innovative crops through the molecular analysis of useful genes” project grant from the National Agriculture and Food Research Organization.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Shingo Nakamura
    • 1
  • Makiko Chono
    • 1
  • Fumitaka Abe
    • 1
  • Hideho Miura
    • 2
  1. 1.National Institute of Crop Science (NICS)TsukubaJapan
  2. 2.Department of Crop ScienceObihiro University of Agriculture and Veterinary MedicineObihiroJapan

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