, Volume 166, Issue 2, pp 219–227 | Cite as

Marker selection for Fusarium head blight resistance based on quantitative trait loci (QTL) from two European sources compared to phenotypic selection in winter wheat

  • T. Miedaner
  • F. Wilde
  • V. Korzun
  • E. Ebmeyer
  • M. Schmolke
  • L. Hartl
  • C. C. Schön


Fusarium head blight (FHB) infects all cereals including maize and is considered a major wheat disease, causing yield losses and mycotoxin contamination. This study aimed to compare the realized selection gain from marker and phenotypic selection in European winter wheat. A double cross (DC) combined three FHB resistance donor-QTL alleles (Qfhs.lfl-6AL and Qfhs.lfl-7BS from ‘Dream’, and one QTL on chromosome 2BL from ‘G16-92’) with two high yielding, susceptible winter wheats, ‘Brando’ and ‘LP235.1’. The base population of 600 DC derived F1 lines was on one hand selected for the respective QTLs by SSR markers (marker-selected cycle, CM), resulting in 35 progeny possessing different combinations of beneficial donor-QTL alleles. On the other hand it was selected phenotypically, only by FHB rating, and the best 20 lines were recombined and selfed (phenotypically selected cycle, CP). The variants CP, CM, and an unselected variant (C0) were tested at four locations by inoculation of Fusarium culmorum. Resistance was measured as the mean of multiple FHB ratings (0–100%). FHB severity was reduced through both phenotypic and marker selection by 6.2 vs. 5.0%, respectively. On a per-year basis, marker selection by 2.5% was slightly superior to phenotypic selection with 2.1%, because the first variant saved 1 year. Marker-selected lines were on average 8.6 cm taller than phenotypically selected lines. A high genetic variation within the marker-selected variant for FHB resistance and the high effect of a resistance-QTL allele on straw length indicate that additional phenotypic selection will further enhance selection gain.


Fusarium culmorum Triticum aestivum Deoxynivalenol Marker QTL Resistance Plant height 



We thank O. Kram, M. Raith, Stefanie Sabrowski, Bianca Schneider, and Meike Scholz for their excellent technical assistance in data collection. This project was supported by the German Federal Ministry of Education and Research (BMBF, Bonn; FKZ 0312559) and the Lochow-Petkus GmbH within the German–French EUREKA Consortium (Project No. Σ! 2386).


  1. Bai G, Shaner G (1994) Scab of wheat: prospects for control. Plant Dis 78:760–766Google Scholar
  2. Bürstmayr H, Lemmens M, Berlakovich S, Ruckenbauer P (1999) Combining ability of resistance to head blight caused by Fusarium culmorum (W.G. Smith) in the F1 of a seven parent diallel of winter wheat (Triticum aestivum L.). Euphytica 110:199–206. doi: 10.1023/A:1003757002052 CrossRefGoogle Scholar
  3. Bürstmayr H, Lemmens M, Hartl L, Doldi L, Steiner B, Stierschneider M, Ruckenbauer P (2002) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (type II resistance). Theor Appl Genet 104:84–91. doi: 10.1007/s001220200009 CrossRefGoogle Scholar
  4. Bürstmayr H, Steiner B, Hartl L, Griesser M, Angerer N, Lengauer D, Miedaner T, Schneider B, Lemmens M (2003) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat II. Resistance to fungal penetration and spread. Theor Appl Genet 107:503–508. doi: 10.1007/s00122-003-1272-6 CrossRefGoogle Scholar
  5. Dubcovsky J (2004) Marker-assisted selection in public breeding programs: the wheat experience. Crop Sci 44:1895–1898Google Scholar
  6. Falconer DS, Mackay TFC (1996) Introduction of quantitative genetics, 4th edn. Longman, New York, 464 ppGoogle Scholar
  7. Fehr WR (1987) Principles of cultivar development, theory and technique, vol 1. Macmillan, New YorkGoogle Scholar
  8. Gale MD, Youssefian S (1985) Dwarfing genes in wheat. In: Russell GE (ed) Progress in plant breeding. Butterworths and Co., London, pp 11–35Google Scholar
  9. Häberle J, Schmolke M, Schweizer G, Korzun V, Ebmeyer E, Zimmermann G, Hartl L (2007) Effects of two major Fusarium head blight resistance QTL verified in a winter wheat backcross population. Crop Sci 47:1823–1831CrossRefGoogle Scholar
  10. Hospital F, Moreau L, Lacoudre F, Charcosset A, Gallais A (1997) More of the efficiency of marker-assisted selection. Theor Appl Genet 95:1181–1189. doi: 10.1007/s001220050679 CrossRefGoogle Scholar
  11. Kuchel H, Ye GY, Fox R, Jefferies S (2005) Genetic and economic analysis of a targeted marker-assisted wheat breeding strategy. Mol Breed 16:67–78. doi: 10.1007/s11032-005-4785-7 CrossRefGoogle Scholar
  12. McMullen M, Jones R, Gallenberg D (1997) Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis 81:1340–1348. doi: 10.1094/PDIS.1997.81.12.1340 CrossRefGoogle Scholar
  13. Mesterházy Á (2003) Control of Fusarium head blight of wheat by fungicides. In: Leonard KJ, Bushnell WR (eds) Fusarium head blight of wheat and barley. The American Phytopathological Society, St. Paul, pp 363–380Google Scholar
  14. Miedaner T, Gang G, Geiger HH (1996) Quantitative-genetic basis of aggressiveness of 42 isolates of Fusarium culmorum for winter rye head blight. Plant Dis 80:500–504Google Scholar
  15. Miedaner T, Reinbrecht C, Lauber U, Schollenberger M, Geiger HH (2001) Effects of genotype and genotype × environment interaction on deoxynivalenol accumulation and resistance to Fusarium head blight in rye, triticale, and wheat. Plant Breed 120:97–105. doi: 10.1046/j.1439-0523.2001.00580.x CrossRefGoogle Scholar
  16. Miedaner T, Schneider B, Oettler G (2006) Means and variances for Fusarium head blight resistance of F2-derived bulks from winter triticale and winter wheat crosses. Euphytica 152:405–411. doi: 10.1007/s10681-006-9228-y CrossRefGoogle Scholar
  17. Moreau L, Lamarie S, Charcosset A, Gallais A (2000) Economic efficiency of one cycle of marker-assisted selection. Crop Sci 40:329–337Google Scholar
  18. SAS Institute (2004) SAS/STAT user’s guide, Version 802. Cary, NC, USAGoogle Scholar
  19. Schmolke M, Zimmermann G, Bürstmayr H, Schweizer G, Miedaner T, Korzun V, Ebmeyer E, Hartl L (2005) Molecular mapping of Fusarium head blight resistance in the winter wheat population Dream/Lynx. Theor Appl Genet 111:747–756. doi: 10.1007/s00122-005-2060-2 PubMedCrossRefGoogle Scholar
  20. Schmolke M, Zimmermann G, Schweizer G, Miedaner T, Korzun V, Hartl L (2008) Molecular mapping of quantitative trait loci for field resistance to Fusarium head blight in a European winter wheat population. Plant Breed 127:459–464. doi: 10.1111/j.1439-0523.2007.01486.x CrossRefGoogle Scholar
  21. Schroeder HW, Christensen JJ (1963) Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53:831–838Google Scholar
  22. Searle SR (1971) Linear models. Wiley, New YorkGoogle Scholar
  23. Snedecor GW, Cochran WG (1989) Statistical methods, 8th edn. Iowa State University, AmesGoogle Scholar
  24. Snijders CHA (1990) Response to selection in F2 generations of winter wheat for resistance to head blight caused by Fusarium culmorum. Euphytica 50:163–169. doi: 10.1007/BF00023641 CrossRefGoogle Scholar
  25. Utz HF (2004) Ein Computerprogramm zur statistischen Analyse pflanzenzüchterischen Experimenten, Version 2O. Institute of Plant Breeding, Seed Science, and Population Genetics. Universität HohenheimGoogle Scholar
  26. Wilde F, Korzun V, Ebmeyer E, Geiger HH, Miedaner T (2007) Comparison of phenotypic and marker-based selection for Fusarium head blight resistance and DON content in spring wheat. Mol Breed 19:357–370. doi: 10.1007/s11032-006-9067-5 CrossRefGoogle Scholar
  27. Wilde F, Schoen CC, Korzun V, Ebmeyer E, Schmolke M, Hartl L, Miedaner T (2008) Marker-based introduction of three quantitative-trait loci conferring resistance to Fusarium head blight into an independent elite winter wheat breeding population. Theor Appl Genet 117:29–35. doi: 10.1007/s00122-008-0749-8 PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • T. Miedaner
    • 1
  • F. Wilde
    • 1
  • V. Korzun
    • 2
  • E. Ebmeyer
    • 2
  • M. Schmolke
    • 3
  • L. Hartl
    • 4
  • C. C. Schön
    • 3
  1. 1.State Plant Breeding InstituteUniversität Hohenheim (720)StuttgartGermany
  2. 2.KWS LOCHOW GMBHBergenGermany
  3. 3.Chair of Plant BreedingTechnische Universität MünchenFreisingGermany
  4. 4.Bavarian State Research Center for Agriculture Institute for Crop Science and Plant BreedingFreisingGermany

Personalised recommendations