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Euphytica

, Volume 166, Issue 2, pp 219–227 | Cite as

Marker selection for Fusarium head blight resistance based on quantitative trait loci (QTL) from two European sources compared to phenotypic selection in winter wheat

  • T. Miedaner
  • F. Wilde
  • V. Korzun
  • E. Ebmeyer
  • M. Schmolke
  • L. Hartl
  • C. C. Schön
Article

Abstract

Fusarium head blight (FHB) infects all cereals including maize and is considered a major wheat disease, causing yield losses and mycotoxin contamination. This study aimed to compare the realized selection gain from marker and phenotypic selection in European winter wheat. A double cross (DC) combined three FHB resistance donor-QTL alleles (Qfhs.lfl-6AL and Qfhs.lfl-7BS from ‘Dream’, and one QTL on chromosome 2BL from ‘G16-92’) with two high yielding, susceptible winter wheats, ‘Brando’ and ‘LP235.1’. The base population of 600 DC derived F1 lines was on one hand selected for the respective QTLs by SSR markers (marker-selected cycle, CM), resulting in 35 progeny possessing different combinations of beneficial donor-QTL alleles. On the other hand it was selected phenotypically, only by FHB rating, and the best 20 lines were recombined and selfed (phenotypically selected cycle, CP). The variants CP, CM, and an unselected variant (C0) were tested at four locations by inoculation of Fusarium culmorum. Resistance was measured as the mean of multiple FHB ratings (0–100%). FHB severity was reduced through both phenotypic and marker selection by 6.2 vs. 5.0%, respectively. On a per-year basis, marker selection by 2.5% was slightly superior to phenotypic selection with 2.1%, because the first variant saved 1 year. Marker-selected lines were on average 8.6 cm taller than phenotypically selected lines. A high genetic variation within the marker-selected variant for FHB resistance and the high effect of a resistance-QTL allele on straw length indicate that additional phenotypic selection will further enhance selection gain.

Keywords

Fusarium culmorum Triticum aestivum Deoxynivalenol Marker QTL Resistance Plant height 

Notes

Acknowledgments

We thank O. Kram, M. Raith, Stefanie Sabrowski, Bianca Schneider, and Meike Scholz for their excellent technical assistance in data collection. This project was supported by the German Federal Ministry of Education and Research (BMBF, Bonn; FKZ 0312559) and the Lochow-Petkus GmbH within the German–French EUREKA Consortium (Project No. Σ! 2386).

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • T. Miedaner
    • 1
  • F. Wilde
    • 1
  • V. Korzun
    • 2
  • E. Ebmeyer
    • 2
  • M. Schmolke
    • 3
  • L. Hartl
    • 4
  • C. C. Schön
    • 3
  1. 1.State Plant Breeding InstituteUniversität Hohenheim (720)StuttgartGermany
  2. 2.KWS LOCHOW GMBHBergenGermany
  3. 3.Chair of Plant BreedingTechnische Universität MünchenFreisingGermany
  4. 4.Bavarian State Research Center for Agriculture Institute for Crop Science and Plant BreedingFreisingGermany

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