Euphytica

, Volume 164, Issue 2, pp 365–375 | Cite as

Development and characterisation of a Brassica carinata inbred line incorporating genes for low glucosinolate content from B. juncea

  • Angustias Márquez-Lema
  • José M. Fernández-Martínez
  • Begoña Pérez-Vich
  • Leonardo Velasco
Article
First Online:
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Accepted:

Abstract

The presence of high levels of sinigrin in the seeds represents a serious constraint for the commercial utilisation of Ethiopian mustard (Brassica carinata A. Braun) meal. The objective of this research was the introgression of genes for low glucosinolate content from B. juncea into B. carinata. BC1F1 seed from crosses between double zero B. juncea line Heera and B. carinata line N2-142 was produced. Simultaneous selection for B. carinata phenotype and low glucosinolate content was conducted from BC1F2 to BC1F4 plant generations. Forty-three BC1F4 derived lines were selected and subject to a detailed phenotypic and molecular evaluation to identify lines with low glucosinolate content and genetic proximity to B. carinata. Sixteen phenotypic traits and 80 SSR markers were used. Eight BC1F4 derived lines were very close to N2-142 both at the phenotypic and molecular level. Three of them, with average glucosinolate contents from 52 to 61 micromoles g−1, compared to 35 micromoles g−1 for Heera and 86 micromoles g−1 for N2-142, were selected and evaluated in two additional environments, resulting in average glucosinolate contents from 43 to 56 micromoles g−1, compared to 29 micromoles g−1 for Heera and 84 micromoles g−1 for N2-142. The best line (BCH-1773), with a glucosinolate profile made up of sinigrin (>95%) and a chromosome number of 2n = 34, was further evaluated in two environments (field and pots in open-air conditions). Average glucosinolate contents over the four environments included in this research were 42, 31 and 74 micromoles g−1 for BCH-1773, Heera and N2-142, respectively. These are the lowest stable levels of glucosinolates reported so far in B. carinata.

Keywords

Brassica carinata Brassica juncea Sinigrin Interspecific crosses Phenotypic traits Simple sequence repeats (SSRs) 
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Notes

Acknowledgements

The authors thank Prof. Dr. Heiko C. Becker and Dr. Christian Möllers, Institute of Agronomy and Plant Breeding, Georg-August University of Göttingen, Germany, for facilities given at the initial steps of this research, and Prof. Dr. A. Martín, Institute for Sustainable Agriculture (CSIC), Córdoba, Spain, for his kind assistance in chromosome counting. The research was partly supported by project MCYT AGL2001-2293 of the Spanish Government.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Angustias Márquez-Lema
    • 1
  • José M. Fernández-Martínez
    • 1
  • Begoña Pérez-Vich
    • 1
  • Leonardo Velasco
    • 1
  1. 1.Institute for Sustainable Agriculture (CSIC)CórdobaSpain

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