Euphytica

, Volume 161, Issue 3, pp 413–427

A preliminary genetic analysis of fibre traits and the use of new genomic SSRs for genetic diversity in jute

  • Reyazul R. Mir
  • Sachin Rustgi
  • Shailendra Sharma
  • Ravinder Singh
  • Aakash Goyal
  • Jitendra Kumar
  • Anupama Gaur
  • Akhilesh K. Tyagi
  • Haseena Khan
  • Mohit K. Sinha
  • Harindra S. Balyan
  • Pushpendra Kumar Gupta
Article

DOI: 10.1007/s10681-007-9597-x

Cite this article as:
Mir, R.R., Rustgi, S., Sharma, S. et al. Euphytica (2008) 161: 413. doi:10.1007/s10681-007-9597-x

Abstract

Jute is one of the most important fibre crops, which is second only to cotton in providing environment-friendly (biodegradable and renewable) ligno-cellulose fibre. In order to improve this largely neglected crop, we conducted a preliminary study involving the following: (i) analysis of nature and extent of the genetic variability for fibre yield and four other related traits in a set of 81 genotypes belonging to two commercially cultivated Corchorus species (45 genotypes of C. olitorius + 36 genotypes of C. capsularis), (ii) development and analysis of a set of simple sequence repeat (SSR) markers from C. olitorius, and (iii) use of a sub-set of SSRs for assessment of genetic diversity in the above set of 81 genotypes. The results suggested quantitative nature of fibre yield and other related traits, with a preponderance of dominance component in genetic variance. A sub-set of 45 SSRs derived from C. olitorius, when used for a study of DNA polymorphism and genetic diversity, showed high transferability of these C. olitorius SSRs to C. capsularis. The average number of alleles for individual SSRs was surprisingly low (3.04 for both species, 2.02 for C. capsularis and 2.51 for C. olitorius), and so was the average polymorphic information content (PIC; 0.23 and 0.24 in two species). In the dendrogram obtained using a similarity matrix, the 81 genotypes were grouped into three clusters, which largely corresponded to the two species, Cluster I belonging mainly to C. capsularis and the other two closely related clusters (clusters II and III) belonging to C. olitorius. It was also shown that a minimum of 15 SSRs could give the same information as 41 SSRs, thus making many SSRs redundant. The SSR markers developed during the present study and to be developed in future will prove useful not only for evaluation of genetic diversity, but also for molecular mapping/QTL analysis, and for comparative genome analysis of the two Corchorus species.

Keywords

Jute Corchorus Genetic analysis Simple sequence repeats Genetic diversity 

Supplementary material

10681_2007_9597_MOESM1_ESM.doc (132 kb)
(DOC 132 kb)

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Reyazul R. Mir
    • 1
  • Sachin Rustgi
    • 1
  • Shailendra Sharma
    • 1
  • Ravinder Singh
    • 1
  • Aakash Goyal
    • 1
  • Jitendra Kumar
    • 1
  • Anupama Gaur
    • 2
  • Akhilesh K. Tyagi
    • 2
  • Haseena Khan
    • 3
  • Mohit K. Sinha
    • 4
  • Harindra S. Balyan
    • 1
  • Pushpendra Kumar Gupta
    • 1
  1. 1.Molecular Biology Laboratory, Department of Genetics and Plant BreedingCh. Charan Singh UniversityMeerutIndia
  2. 2.Department of Plant Molecular BiologyUniversity of Delhi South CampusNew DelhiIndia
  3. 3.Department of Biochemistry and Molecular BiologyUniversity of DhakaDhakaBangladesh
  4. 4.Division of Crop ImprovementCentral Research Institute for Jute and Allied FibresBarrackporeIndia

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