, Volume 162, Issue 2, pp 231–239 | Cite as

Mapping QTL tolerance to Phytophthora root rot in soybean using microsatellite and RAPD/SCAR derived markers

  • Yingpeng Han
  • Weili Teng
  • Kangfu Yu
  • Vaino Poysa
  • Terry Anderson
  • Lijuan Qiu
  • David A. Lightfoot
  • Wenbin Li


Broad tolerance to phytophthora root rot (PRR) caused by Phytophthora sojae has become an important goal for the improvement of soybean (Glycine max) because of the rapid spread of races that defeat the available resistance genes. The aim of this research was to identify the location of quantitative trait loci (QTL) in ‘Conrad’, a soybean cultivar with broad tolerance to many races of P. sojae. A PRR susceptible breeding line ‘OX760-6-1’was crossed with Conrad. Through single-seed-descent, 112, F2 derived, F7 recombinant inbred lines (RILs) were advanced. A total of 39 random amplified polymorphic DNA bands (RAPDs) and 89 type 1 microsatellite (simple sequence repeat; SSR) markers were used to construct a genetic linkage map. In the greenhouse, RILs were inoculated with four P. sojae isolates (three from China and one from Canada). Disease was measured as the percent of dead plants 20 days after germination in P. sojae inoculated vermiculite in the greenhouse. Three QTLs (QGP1, QGP2, QGP3) for PRR tolerance in the greenhouse were detected using WinQTLCart 2.0 with a log-likelihood (LOD) score 27.14 acquired through permutations (1,000 at P ≤ 0.05). QGP1 (near Satt509) was located at linkage group F and explained 13.2%, 5.9%, and 6.7% of the phenotypic variance for tolerance to the JiXi, JianSanJiang and ShuangYaShan isolates, respectively. QGP2 (near Satt334) was located in a different interval on linkage group F and explained 5.1% and 2.4% of the phenotypic variance for JiXi and ShuangYaShan isolates, respectively. QGP3 was located on linkage group D1b + W (near OPL18800/SCL18659) and explained 10.2% of the phenotypic variance for Woodslee isolate. QGP1 and QGP2 appeared to be associated with PRR tolerance across a range of isolates but QGP3 was active only against the Woodslee isolate. At Woodslee and Weaver (in Ontario) in 2000, the interval associated with QGP3 explained 21.6% and 16.7% of phenotypic variance in resistance to PRR, respectively and was referred as QFP1. The identified QTLs would be beneficial for marker assistant selection of PRR tolerance varieties against both China and North America P. sojae races.


Quantitative trait loci SSR marker SCAR marker Marker-assistant selection Phytophthora root rot Soybean 



This project was conducted in the Key Laboratory of Soybean Biology of Chinese Education Ministry and financially supported by National High Technology Program (2006AA100104-4, 2006AA10Z1F1), National International Cooperation Project, National Science Foundation as well by Ontario Soybean Growers. The technical assistance of Chuck Meharg and Elaine Lepp is gratefully acknowledged.


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Yingpeng Han
    • 1
  • Weili Teng
    • 1
  • Kangfu Yu
    • 2
  • Vaino Poysa
    • 2
  • Terry Anderson
    • 2
  • Lijuan Qiu
    • 3
  • David A. Lightfoot
    • 4
  • Wenbin Li
    • 1
  1. 1.Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry Northeast Agricultural UniversityHarbinChina
  2. 2.Greenhouse and Processing Crops Research CentreAgriculture and Agri-Food CanadaHarrowCanada
  3. 3.Institute of Crop ScienceChinese Academy of AgricultureBeijingChina
  4. 4.Centers for Excellence in Soybean ResearchSouthern Illinois UniversityCarbondaleUSA

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