Euphytica

, Volume 162, Issue 2, pp 231–239 | Cite as

Mapping QTL tolerance to Phytophthora root rot in soybean using microsatellite and RAPD/SCAR derived markers

  • Yingpeng Han
  • Weili Teng
  • Kangfu Yu
  • Vaino Poysa
  • Terry Anderson
  • Lijuan Qiu
  • David A. Lightfoot
  • Wenbin Li
Article

Abstract

Broad tolerance to phytophthora root rot (PRR) caused by Phytophthora sojae has become an important goal for the improvement of soybean (Glycine max) because of the rapid spread of races that defeat the available resistance genes. The aim of this research was to identify the location of quantitative trait loci (QTL) in ‘Conrad’, a soybean cultivar with broad tolerance to many races of P. sojae. A PRR susceptible breeding line ‘OX760-6-1’was crossed with Conrad. Through single-seed-descent, 112, F2 derived, F7 recombinant inbred lines (RILs) were advanced. A total of 39 random amplified polymorphic DNA bands (RAPDs) and 89 type 1 microsatellite (simple sequence repeat; SSR) markers were used to construct a genetic linkage map. In the greenhouse, RILs were inoculated with four P. sojae isolates (three from China and one from Canada). Disease was measured as the percent of dead plants 20 days after germination in P. sojae inoculated vermiculite in the greenhouse. Three QTLs (QGP1, QGP2, QGP3) for PRR tolerance in the greenhouse were detected using WinQTLCart 2.0 with a log-likelihood (LOD) score 27.14 acquired through permutations (1,000 at P ≤ 0.05). QGP1 (near Satt509) was located at linkage group F and explained 13.2%, 5.9%, and 6.7% of the phenotypic variance for tolerance to the JiXi, JianSanJiang and ShuangYaShan isolates, respectively. QGP2 (near Satt334) was located in a different interval on linkage group F and explained 5.1% and 2.4% of the phenotypic variance for JiXi and ShuangYaShan isolates, respectively. QGP3 was located on linkage group D1b + W (near OPL18800/SCL18659) and explained 10.2% of the phenotypic variance for Woodslee isolate. QGP1 and QGP2 appeared to be associated with PRR tolerance across a range of isolates but QGP3 was active only against the Woodslee isolate. At Woodslee and Weaver (in Ontario) in 2000, the interval associated with QGP3 explained 21.6% and 16.7% of phenotypic variance in resistance to PRR, respectively and was referred as QFP1. The identified QTLs would be beneficial for marker assistant selection of PRR tolerance varieties against both China and North America P. sojae races.

Keywords

Quantitative trait loci SSR marker SCAR marker Marker-assistant selection Phytophthora root rot Soybean 

Notes

Acknowledgement

This project was conducted in the Key Laboratory of Soybean Biology of Chinese Education Ministry and financially supported by National High Technology Program (2006AA100104-4, 2006AA10Z1F1), National International Cooperation Project, National Science Foundation as well by Ontario Soybean Growers. The technical assistance of Chuck Meharg and Elaine Lepp is gratefully acknowledged.

References

  1. Abney T, Melgar J, Richards T, Scott D, Grogan J, Young J (1997) New races of Phytophthora sojae with Rps1-d virulence. Plant Dis 81:653–655CrossRefGoogle Scholar
  2. Bernard R, Smith P, Kaufmann M, Schmitthenner A (1957) Inheritance of resistance to Phytophthora root rot and stem rot in the soybean. Agron J 49:391CrossRefGoogle Scholar
  3. Bhat R, Schmitthenner A (1993) Genetic crosses between physiologic races of Phytophthora sojae. Exp Mycol 17:122–129CrossRefGoogle Scholar
  4. Burnham K, Dorrance A, Francis D, Fioritto R, St. Martin S (2003a) Rps8, A new locus in soybean for resistance to Phytophthora sojae. Crop Sci 43:101–105CrossRefGoogle Scholar
  5. Burnham K, Dorrance A, VanToai T, St. Martin S (2003b) Quantitative trait loci for partial resistance to Phytophthora sojae in soybean. Crop Sci 43:1609–1617Google Scholar
  6. Buzzell R, Anderson T (1982) Plant loss response of soybean cultivars to Phytophthora megasperma f.sp. glycinea under field conditions. Plant Dis 66:1146–1148CrossRefGoogle Scholar
  7. Cregan P, Jarvik T, Bush A, Shoemaker R, Lara K, Kahler A, Kaya N, VanToai T, Lohnes D, Chung J, Specht J (1999) An integrated genetic linkage map of the soybean genome. Crop Sci 39:1464–1490CrossRefGoogle Scholar
  8. Demirbas A., Rector B, Lohnes D, Fioritto R, Graef G, Cregan P, Shoemaker R, Specht J (2001) Simple sequence repeat markers linked to the soybean Rps Genes for Phytophthora resistance. Crop Sci 41:1220–1227CrossRefGoogle Scholar
  9. Diers B, Mausur L, Imsanda J, Shoemaker R (1992) Mapping Phytophthora resistance loci in soybean with restriction fragment length polymorphism markers. Crop Sci 32:377–383CrossRefGoogle Scholar
  10. Dorrance A and St. Martin S (2000) Phytophthora sojae: is it time for a new approach? APS net pathogen population genetics and breeding for disease resistance http://www.apsnet.org/online/feature/PathPopGenetics/dorrance.html
  11. Drenth A, Whisson S, MacLean D, Irwin J, Obst N, Riley M (1996) The evolution of Phytophthora sojae in Australia. Phytopathology 86(2):163–169CrossRefGoogle Scholar
  12. Fehr W, Cianzio S, Voss B, Schultz S (1989) Registration of ‘Conrad’ soybean. Crop Sci 29:830CrossRefGoogle Scholar
  13. Gebhardt C, Valkonen J (2001) Organization of genes controlling disease resistance in the potato genome. Annu Rev Phytopathol 39:79–102PubMedCrossRefGoogle Scholar
  14. Hartman G., Sinclair J, Rupe J (1999) A compendium of soybean diseases 4th edn. The American Phytopathological Society, St Paul, MN 100Google Scholar
  15. Hildebrand A (1959) A root and stalk rot of soybean caused by Phytophthora megaspema var. sojae var. nov. Can J Bot 37:927–957CrossRefGoogle Scholar
  16. Hyten D, Pantalone V, Sams C, Saxton A, Landau-Ellis D, Stefaniak T, Schmidt M (2004) Seed quality QTL in a prominent soybean population. Theor Appl Genet 109:552–561PubMedCrossRefGoogle Scholar
  17. Irwin J, Cahill D, Drenth A (1995) Phytophthora in Australia. Aust J Agric Res 46:1311–1337CrossRefGoogle Scholar
  18. Jee H, Kim W, Cho W (1998) Occurrence of Phytophthora root rot on soybean (Glycine max) and identification of the causal fungus. Crop Prot 40:16–22Google Scholar
  19. Jones J, Johnson H (1969) Lupine, a new host for Phytophthora megasperma var. sojae. Phytopathology 59:504–507Google Scholar
  20. Kaufmann M, Gerdemann W (1958) Root and stem rot of soybean caused by Phytophthora sojae n. sp. Phytopathology 48:201–208Google Scholar
  21. Lander E, Green P, Abrahamson J, Baarlow A, Daly M, Lincoln S, Newburg L (1987) MapMaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181PubMedCrossRefGoogle Scholar
  22. Leitz R, Hartman G, Pederson W, Nickell C (2000) Races of Phytophthora sojae on soybean in Illinois. Plant Dis 84:487CrossRefGoogle Scholar
  23. Lohnes D, Nickell C, Schmitthenner A et al (1996) Origin of soybean alleles for Phytophthora resistance in China. Crop Sci 36:1689–1692CrossRefGoogle Scholar
  24. Malvick D, Grunden E (2004) Traits of soybean-infecting Phytophthora populations from Illinois agricultural fields. Plant Dis 88:1139–1145CrossRefGoogle Scholar
  25. Meng X, Shoemaker R, Yang B (1999) Analysis of pathogenicity and genetic variation among phytophthtora sojae isolates using RAPD. Mycol 103:173–178CrossRefGoogle Scholar
  26. Rafalski J, Tingey S, William J (1991) RAPD markers-a new technology for genetic mapping and plant breeding. AgBiotech News and Information 3:645–648Google Scholar
  27. Rizvi S, Yang X (1996) Fungi associated with soybean seeding diseases in Iowa. Plant Disease .80:57–60CrossRefGoogle Scholar
  28. Ryley M, Obst N, Irwin J, Drenth A (1998) Changes in the racial composition of Phytophthora sojae in Australia between 1979 and 1996. Plant Dis 82:1048–1054CrossRefGoogle Scholar
  29. Sandhu D, Schallock K, Rivera-velez N, Lundeen P, Cianzio S, Bhattacharyya M (2005) Soybean Phytophthora resistance gene Rps8 maps closely to the Rps3 region. Hered 96(5):536–541CrossRefGoogle Scholar
  30. Schafer J (1971) Tolerance to plant disease. Annu Rev Phytopathol 9:235–252CrossRefGoogle Scholar
  31. Schmitthenner A (1989) Phytophthora root rot. In: Sinclair JB, Backman PA (eds) Compendium of soybean diseases. APS Press, St Paul, Minnesota, 35–38Google Scholar
  32. Song Q, Marek L, Shoemaker R, Lark K, Concibido V, Delannay X, Specht J, Cregan P (2004) A new integrated genetic linkage map of the soybean. Theor Appl Genet 109:122–128PubMedCrossRefGoogle Scholar
  33. Su Y, Yao S (1993) The discovery and biological characteristics studies of Phytophthora megasperma f.sp.glycinea on soybean in China. Acta Phytopathol Sin 23:341–347Google Scholar
  34. Thomison P, Thomas C, Kenworthy W, McIntosh M (1988) Evidence of pathogen specify in tolerance of soybean cultivars to phytophthora root rot. Crop Sci 28:714–715CrossRefGoogle Scholar
  35. Tooley P, Grau C (1982) Identification and quantitative characterization of rate-reducing resistance to Phytophthora megasperma f. sp. glycinea in soybean seedlings. Phytopathology 72:727–733CrossRefGoogle Scholar
  36. Tooley P, Grau C (1984a) Field characterization of rate-reducing resistance to Phytophthora megasperma f. sp. glycinea in soybean. Phytopathology 74:1201–1208Google Scholar
  37. Tooley P, Grau C (1984b) The relationship between rate-reducing resistance to Phytophthora megasperma f.sp. glycinea and yield of soybean. Phytopathology 74:1209–1216Google Scholar
  38. Trigiano R, Caetano-Anolles G (1998) Laboratory exercises on DNA amplification fingerprinting for evaluating the molecular diversity of horticultural species. Hort Technol 8:413–423Google Scholar
  39. Voorrips R (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78PubMedCrossRefGoogle Scholar
  40. Walker A, Schmitthenner A (1984) Heritability of tolerance to Phytophthora root rot in soybean. Crop Sci 24:490–491CrossRefGoogle Scholar
  41. Wang Z, Wang Y, Zhang Z, Zheng X (2006) Comparison analysis of Phytophthora sojae between China and America. Chin Sci Bull 51(16):1913–1919CrossRefGoogle Scholar
  42. Weng C, Yu K, Anderson T, Poysa V (2001) Mapping genes conferring resistance to phytophthora root rot of soybean, Rps1a and Rps7. Hered 92(5):442–446CrossRefGoogle Scholar
  43. Wrather J, Anderson T, Arsyad D, Tan Y, Ploper L, Porta-Puglia A, Ram H, Yorinori J (2001) Soybean disease loss estimates for the top ten soybean producing countries in 1998. Can J Plant Pathol 23:115–121CrossRefGoogle Scholar
  44. Xu Y, Han X, Zhang Z, Zhong Z, He Z, Yang Q (2000) Main pathogens and pests in Heilongjiang Provine. Crop Mag 5:9–10Google Scholar
  45. Yan W (2001) GGE biplot-a windows application for graphical analysis of multi-environment trial data and other types of two way data. Agron J 93:1111–1117CrossRefGoogle Scholar
  46. Yu K, Park S, Poysa V (1999) Abundance and variation of microsatellite DNA sequences in beans (Phaseolus and Vigna). Genome 42:27–34CrossRefGoogle Scholar
  47. Zeng Z (1993) Theoretical basis of separation of multiple linked gene effects on mapping quantitative trait loci. Proc Natl Acad Sci USA 90:10972–10976PubMedCrossRefGoogle Scholar
  48. Zheng C, Chang R, Qiu L, Chen P, Wu X, Chen S (2003) Identification and characterization of a RAPD/SCAR marker linked to a resistance gene for soybean mosaic virus in soybean. Euphytica 132:199–210CrossRefGoogle Scholar
  49. Zhu Z, Huo Y, Wang X, Huang J, Wu X (2004) Analysis of simple sequence repeats markers derived from Phytophthora sojae expressed sequence tags. Chin Sci Bull 49(19):2041–2046CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Yingpeng Han
    • 1
  • Weili Teng
    • 1
  • Kangfu Yu
    • 2
  • Vaino Poysa
    • 2
  • Terry Anderson
    • 2
  • Lijuan Qiu
    • 3
  • David A. Lightfoot
    • 4
  • Wenbin Li
    • 1
  1. 1.Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry Northeast Agricultural UniversityHarbinChina
  2. 2.Greenhouse and Processing Crops Research CentreAgriculture and Agri-Food CanadaHarrowCanada
  3. 3.Institute of Crop ScienceChinese Academy of AgricultureBeijingChina
  4. 4.Centers for Excellence in Soybean ResearchSouthern Illinois UniversityCarbondaleUSA

Personalised recommendations