Morphological and physiological behaviour of sweet cherry ‘somaclone’ HS plants in field
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We reported earlier that somaclonal variant HS (HS), regenerated from Prunus avium `Hedelfinger’ (H) leaf explants, showed reduced apical dominance both in vitro and in vivo. In this report we found that the somaclone HS, showed reduced vegetative vigour as determined by trunk circumference and tree height nevertheless natural architecture of the tree was preserved. HS canopy was less crowded as to compare to H and leaves were shorter with a shorter petiole, they were better spaced out as indicated by internodes length. HS had a significantly thinner cuticle and wax layer compared to H leaves surface. Histological analysis of leaves also showed that HS had thicker mesophyll and palisade the latter composed of more than one layer which, together with increased stomatal density, are distinguishing signs of extensive exposure of leaves to sun. Chlorophyll a and chlorophyll b content did not differ between H and HS, β-carotene content was slightly lower in HS leaves. However no significant differences were found between the two genotypes in terms of net photosynthesis, intercellular CO2 concentration, leaf transpiration and water use efficiency. HS flowered 1-year later than H and number of flowers was reduced. Fruit size, shape, skin colour change during the ripening process and the main parameters of fruit quality as assessed in the wild type and the somaclone were basically the same.
KeywordsCuticle Mesophyll Net photosynthesis Stomatal density Tree height Trunk circumference Fruit characteristics
This research has been supported by MIUR-PRIN project ‘Regulation of development and growth habit of crop plants through genetic engineering: regeneration and transformation of sweet cherry (Prunus avium L.) cultivars’. The authors wish to thank the ‘C.L.I.F.O.F. Foundation’ (Minoprio, Italy) for maintaining these tissue culture derived cherry trees and Dr. C. Srinivasan (USDA-ARS Appalachian Fruit Research Station, Kearneysville, WV) for the critical reading of the manuscript.
- Bargioni A (1996) Sweet cherry scions: characteristics of the principal commercial cultivars, breeding objectives and methods. In: Webster AD, Looney NE (eds) Cherries: crop physiology, production and uses. CAB International, Wallingford UKGoogle Scholar
- Bassi D (ed) (2003) Growth habits in stone fruit trees. Il Divulgatore, BolognaGoogle Scholar
- Grbić V (2002) Axillary meristem development. In: McManus MT, Veit B (eds) Meristematic tissues in plant growth and development. Biological sciences series. Sheffield Academic Press, EnglandGoogle Scholar
- Leva AR, Muleo R, Polsinelli L, Petruccelli R, Montagni G (2000) Frangivento olive somaclones originated by somatic embryogenesis show both different polimorphic patterns and growth habit. In: Failla O, Piagnani MC (eds) Proceedings of V Giornate scientifiche SOI, Edizioni Tecnos, Milano Italy, pp 622Google Scholar
- Lüttge U, Kluge M, Bauer G (1997) Botanica. Zanichelli, BolognaGoogle Scholar
- Miller SS, Tworkoski T (2003) Regulating vegetative growth in deciduous fruit trees. Plant Growth Regul Soc Am Q 31(1):8–46Google Scholar
- Piagnani MC, Iacona C, Intrieri MC, Muleo R (2005) A Somaclonal variant in ‘Hedelfinger’ sweet cherry. Acta Hortic 667(1):93–99Google Scholar
- Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. BBA 975:384–394CrossRefGoogle Scholar
- Ružić D, Cerovic R, Boskovic R (1991) The assessment of somaclonal variation in sour cherry cv. Sumadinka regenerated from leaf explants. Fruit Sci Rep 18(4):155–162Google Scholar
- Wasmund N, Topp I, Schories D (2006) Optimising the storage and extraction of chlorophyll samples. Oceanologia 48(1):125–144Google Scholar