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Comparative genetic analysis and molecular mapping of fertility restoration genes for WA, Dissi, and Gambiaca cytoplasmic male sterility systems in rice

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Abstract

The genetic relationship among three cytoplasmic male sterility (CMS) systems, consisting of WA, Dissi, and Gambiaca, was studied. The results showed that the maintainers of one CMS system can also maintain sterility in other cytoplasmic backgrounds. The F1 plants derived from crosses involving A and R lines of the respective cytoplasm and their cross-combination with other CMS systems showed similar pollen and spikelet fertility values, indicating that similar biological processes govern fertility restoration in these three CMS systems. The results from an inheritance study showed that the pollen fertility restoration in all three CMS systems was governed by two independent and dominant genes with classical duplicate gene action. Three F2 populations, generated from the crosses between the parents of good-performing rice hybrids, that possess WA, Dissi, and Gambiaca CMS cytoplasm, were used to map the Rf genes. For the WA-CMS system, Rf3 was located at a distance of 2.8 cM from RM490 on chromosome 1 and Rf4 was located at 1.6 cM from RM1108 on chromosome 10. For the Dissi-CMS system, Rf3 was located on chromosome 1 at 1.9 cM from RM7466 and Rf4 on chromosome 10 was located at 2.3 cM from RM6100. The effect of Rf3 on pollen fertility appeared to be stronger than the effect of Rf4. In the Gambiaca-CMS system, only one major locus was mapped on chromosome 1 at 2.1 cM from RM576. These studies have led to the development of marker-assisted selection (MAS) for selecting putative restorer lines, new approaches to alloplasmic line breeding, and the transfer of Rf genes into adapted cultivars through a backcrossing program in an active hybrid rice breeding program.

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Acknowledgments

This research was supported by a graduate program scholarship from IRRI and the Ministry of Agriculture of the Islamic Republic of Iran. The authors thank Dr. Bill Hardy for editing the manuscript and T. M. Nas for technical assistance during the study.

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Authors and Affiliations

  1. Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines

    Majid Sattari, Arumugam Kathiresan, Glenn B. Gregorio & Sant S. Virmani

  2. Rice Research Institute of Iran, P.O. Box 145, Amol, Iran

    Majid Sattari

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  1. Majid Sattari
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  2. Arumugam Kathiresan
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  3. Glenn B. Gregorio
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  4. Sant S. Virmani
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Correspondence to Sant S. Virmani.

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Sattari, M., Kathiresan, A., Gregorio, G.B. et al. Comparative genetic analysis and molecular mapping of fertility restoration genes for WA, Dissi, and Gambiaca cytoplasmic male sterility systems in rice. Euphytica 160, 305–315 (2008). https://doi.org/10.1007/s10681-007-9498-z

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