Euphytica

, Volume 158, Issue 1–2, pp 87–94 | Cite as

A major QTL associated with cold tolerance at seedling stage in rice (Oryza sativa L.)

  • Qiaojun Lou
  • Liang Chen
  • Zongxiu Sun
  • Yongzhong Xing
  • Jun Li
  • Xiaoyan Xu
  • Hanwei Mei
  • Lijun Luo
Article

Abstract

Chilling injury is one of the most important limiting factors affecting rice production in temperate and high-elevation areas. In this study, 146 microsatellite markers were employed to identify quantitative trait loci (QTL) conferring cold tolerance at seedling stage (CTS) .The mapping population consisted of 193 doubled haploid (DH) lines, which derived from a cross between a cold-tolerant japonica variety (AAV002863) and a cold-sensitive indica cultivar (Zhenshan97B). Tolerance to cold was assessed by the survival percentage of seedlings after cold treatment. In a climate chamber, after treatment at 6°C/10°C for 7 d, the measurement was taken on the sixth day of the recovery stage at room temperature. The phenotypic distribution of the DH population approximately fitted normality with skewness and kurtosis less than 0.3, and the difference among the three repetitions was not significant. Five main effect QTLs were identified with LOD > 4.0 on chromosomes 1, 2, 8 using a composite interval mapping approach. The accumulated contribution of the five QTLs was 62.28%, and a major QTL (LOD = 15.09) was identified on chromosome 2 flanked by RM561 and RM341, which explained 27.42% of the total phenotypic variation. Four significant epistatic interactions were also detected with a total contribution of 20.14%.

Keywords

Cold tolerance at seedling stage (CTS) Microsatellite marker (SSR) Quantitative trait locus (QTL) Rice 

Notes

Acknowledgements

This work was supported by the Program of Shanghai Qiming Star (04qmx1458) and the 973 program (2004CB117204).

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Qiaojun Lou
    • 1
  • Liang Chen
    • 1
  • Zongxiu Sun
    • 3
  • Yongzhong Xing
    • 2
  • Jun Li
    • 1
  • Xiaoyan Xu
    • 1
  • Hanwei Mei
    • 1
  • Lijun Luo
    • 1
    • 2
  1. 1.Shanghai Agrobiological Gene CenterShanghaiChina
  2. 2.Huazhong Agriculture UniversityWuhanChina
  3. 3.China National Rice InstituteHangzhouChina

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