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Euphytica

, Volume 155, Issue 3, pp 329–336 | Cite as

Evaluation of leaf rust resistance genes Lr1, Lr9, Lr24, Lr47 and their introgression into common wheat cultivars by marker-assisted selection

  • Francesca Nocente
  • Laura Gazza
  • Marina PasquiniEmail author
Article

Abstract

Epidemiological field controls in different Italian locations and seedling evaluations of the ‘Thatcher’ near-isogenic lines (NILs) carrying the leaf rust resistance genes Lr1, Lr9, Lr24 and Lr47 were conducted during 5 years of testing. These genes confirmed their effectiveness in both field and greenhouse conditions. Moreover a backcross program was carried out by using as recurrent parents the susceptible high-quality common wheat cvs ‘Bolero’, ‘Colfiorito’, ‘Serio’ and ‘Spada’ and the ‘Thatcher’ NILs carrying the above mentioned genes as donor parents. The progenies of different cross combinations were selected by both resistance tests and marker assisted selection using molecular markers (STS, SCAR, CAPS) closely linked to Lr genes: a complete cosegregation was observed between the resistance genes used and the corresponding molecular markers.

Keywords

Common wheat Leaf rust Lr genes Molecular markers Resistance tests 

Notes

Acknowledgements

Research supported by a grant from Mipaaf, Project AMIFRUGAM.

References

  1. Andrivon D, De Vallavieille-Pope C (1993) Racial diversity and complexity in regional populations of Erysiphe graminis f.sp. hordei in France over a 5-year period. Plant Pathol 42:443–464CrossRefGoogle Scholar
  2. Bai D, Knott DR (1992) Suppression of rust resistance in bread wheat (Triticum aestivum L.) by D-genome chromosomes. Genome 35:276–282Google Scholar
  3. Blaszczyk L, Chelkowski J, Korzun V, Kraic J, Ordon F, Ovesna J, Purnhauser L, Tar M, Vida G (2004) Verification of STS markers for leaf rust resistance genes of wheat by seven European laboratories. Cell Mol Biol Lett 9:805–817PubMedGoogle Scholar
  4. Casulli F, Pasquini M (1993) Virulenza delle popolazioni di Puccinia recondita f.sp. tritici e P. graminis f.sp. tritici in Italia. Phytopath Medit 32:115–120Google Scholar
  5. Casulli F, Pasquini M (1998) Pathogenicity of Puccinia recondita f.sp. tritici in Italy from 1993 to 1996. Phytopath Medit 37:51–57Google Scholar
  6. Chelkowski J Golka L, Stepien L (2003) Application of STS markers for leaf rust resistance genes in near-isogenic lines of spring wheat cv. Thatcher. J Appl Genet 44(3):323–338Google Scholar
  7. Dedryver F, Jubier MF, Thouverin J, Goyeau H (1996) Molecular markers linked to leaf rust resistance gene Lr 24. Genome 39:830–835PubMedGoogle Scholar
  8. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II- Plant. Mol Biol Rep 1:19–21CrossRefGoogle Scholar
  9. Dubcovsky J, Lukaszewski AJ, Echaide M, Antonelli EF, Porter DR (1998) Molecular characterization of two Triticum speltoides interstitial translocations carrying leaf rust and greenbug resistance genes. Crop Sci 38:1655–1660CrossRefGoogle Scholar
  10. Feuillet C, Messmer M, Schachermayr G, Keller B (1995) Genetical and physical characterization of Lr1 leaf rust resistance locus in wheat (Triticum aestivum L.). Mol Gen Genet 248:553–562PubMedCrossRefGoogle Scholar
  11. Friebe B, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87Google Scholar
  12. Gupta AK, Saini RG, Gupta S, Malhotra S (1984) Genetic analysis of two wheat cultivars, “Sonalika” and “WL711” for reaction to leaf rust (Puccinia recondita). Theor Appl Genet 67:215–217CrossRefGoogle Scholar
  13. Gupta PK, Varshney RK, Sharma PC, Ramesh B (1999) Molecular markers and their applications in wheat breeding. Plant Breed 118:369–390CrossRefGoogle Scholar
  14. Gupta SK, Charpe A, Koul S, Haque QMR, Prabhu KV (2006) Development and validation of SCAR markers co-segregating with an Agropyron elongatum derived leaf rust resistance gene Lr24 in wheat. Euphytica 150(1–2):233–240CrossRefGoogle Scholar
  15. Helguera M, Khan IA, Dubcovsky J (2000) Development of PCR markers for the wheat leaf rust resistance gene Lr 47. Theor Appl Genet 100:1137–1143CrossRefGoogle Scholar
  16. Helguera M, Khan IA, Kolmer J, Lijavetzky D, Zhong-qi L, Dubcovsky J (2003) PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines. Crop Sci 43:1839–1847CrossRefGoogle Scholar
  17. Korzun V (2002) Use of molecular markers in cereal breeding. Cell Mol Biol Lett 7:811–820PubMedGoogle Scholar
  18. Limpert E, Muller K (1994) Designation of pathotypes of plant pathogens. J Phytopath 140:346–358Google Scholar
  19. Martinez F, Sillero JC, Rubiales D (2005) Pathogenic specialization of Puccinia triticina in Andalusia from 1998 to 2000. J Phytopath 153(6):344–349CrossRefGoogle Scholar
  20. McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO Publications, VIC, Australia, 200 ppGoogle Scholar
  21. McIntosh RA, Hart GE, Devos KM, Morris CF, Rogers WJ (2003) Catalogue of gene symbols for wheat: 2003 Supplement. Ann Wheat Newsl 49:246–282Google Scholar
  22. Mesterhazy A, Bartos P, Goyeau H, Niks RE, Csosz M, Andersen O, Casulli F, Ittu M, Jones E, Manisterski J, Manninger K, Pasquini M, Rubiales D, Schachermayr G, Strzembicka A, Szunics L, Todorova M, Unger O, Vanco B, Vida G, Walther U (2000) European virulence survey for leaf rust in wheat. Agronomie 20:793–804CrossRefGoogle Scholar
  23. Park RF, Felsenstein FG (1998) Physiological specialization and pathotype distribution of Puccinia recondita in western Europe, 1995. Plant Pathol 47(2):157–164CrossRefGoogle Scholar
  24. Pasquini M, Zitelli G (1984) Virulence changes in Puccinia recondita f.sp.tritici and Puccinia graminis tritici in Italy from 1960 to 1983. In: Proceeding of the 6th European and mediterranean cereal rusts conference, Grignon (France), 4–7 September 1984, pp 175–180Google Scholar
  25. Pasquini M, Pancaldi D, Casulli F (2003) Genetic variation in Italian populations of Puccinia recondita f.sp. tritici from 1990 to 2001. J Genet Breed 57:191–200Google Scholar
  26. Pasquini M, Iori A, Sereni L, Casini F, L’Aurora A, Matere A, Nocente F, Gazza L, Siniscalco A, Matteu L, Preiti G, Raimondo I, Randazzo B, Cambrea M, Mameli L, Mayerle M, Viola P, Notario T (2005) Malattie fungine sui frumenti: limitato sviluppo nel 2004–2005. L′Informatore Agrario 36:66–73Google Scholar
  27. Pathan AK, Park RF (2006) Evaluation of seedling and adult plant resistance to leaf rust in European wheat cultivars. Euphytica. DOI 10.1007/s10681-005-9081-4Google Scholar
  28. Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust severity on leaves and stems of cereals. Can J Res Sect C Bot Sci 26:496–500Google Scholar
  29. Roelfs AP (1985a) Wheat and Rye stem rust. In: Roelfs AP, Bushnell WR (eds) The cereal rusts, Vol. 2: diseases, distribution, epidemiology and control. Academic, Orlando, FL, pp 3–27Google Scholar
  30. Roelfs AP (1985b) Epidemiology in North America. In: Roelfs AP, Bushnell WR (eds) The cereal rusts, Vol. 2: diseases, distribution, epidemiology and control. Academic, Orlando, FL, pp 403–434Google Scholar
  31. Roelfs AP (1988) Resistance to leaf and stem rusts in wheat. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to rust of wheat. International Maize and Wheat Improvement Center, Mexico, DF, pp 10–19Google Scholar
  32. Roelfs AP, Sing RP, Saari EE (1992) Rust diseases of wheat: concepts and methods of disease management. International Maize and Wheat Improvement Center, Mexico, DF, pp 10–19Google Scholar
  33. Schachermayr G, Siedler H, Gale MD, Winzeler H, Winzeler M, Keller B (1994) Identification and localization of molecular markers linked to the Lr 9 leaf rust resistance gene of wheat. Theor Appl Genet 88:110–115CrossRefGoogle Scholar
  34. Siniscalco A, Paradies M, Fanelli C, Zitelli G, Biancolatte E, Cecchi V, Ceoloni C, Pasquini M, Vallega V (1978) Risultati delle prove di campo eseguite nel 1977–78 sul comportamento di frumenti verso ruggini e oidio. Bollettino dell’ Istituto Patologia Vegetale, Università di Bari e dell’Istituto sperimentale per la cerealicoltura di Roma. Ottobre 1978, pp 1–57Google Scholar
  35. Winzeler M, Mesterhazy A, Park RF, Bartos P, Csosz M, Goyeau H, Ittu M, Jones E, Loschemberger F., Manninger K, Pasquini M, Richter K, Rubiales D, Schachermayr G, Strzembicka A, Trottet M, Unger O, Vida G, Walther U (2000) Resistance of European winter wheat germoplasm to leaf rust. Agronomie 20:783–792CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Francesca Nocente
    • 1
  • Laura Gazza
    • 1
  • Marina Pasquini
    • 1
    Email author
  1. 1.Istituto Sperimentale per la Cerealicoltura, C.R.A., Section of Applied GeneticsRomeItaly

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