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Euphytica

, Volume 154, Issue 1–2, pp 113–126 | Cite as

Responses to water withdrawal of tobacco plants genetically engineered with the AtTPS1 gene: a special reference to photosynthetic parameters

  • André M. AlmeidaEmail author
  • Anabela B. Silva
  • Susana S. Araújo
  • Luís A. Cardoso
  • Dulce M. Santos
  • José M. Torné
  • Jorge M. Silva
  • Matthew J. Paul
  • Pedro S. Fevereiro
Article

Abstract

We have previously obtained several lines of tobacco transformed with a trehalose-6-phosphate synthase gene of plant origin (Arabidopsis thaliana), involved in the first step of the biosynthesis of trehalose, a known osmoprotectant. Two showed distinct intensity of expression: high (B5H) and low (B1F). Such lines were analyzed for trehalose-6-phosphate content and the obtained results demonstrated to be in accordance with the expression results. In order to study the responses of photosynthesis to water deficit of transgenic lines in comparison to wild type (WT), three experiments were performed under different conditions: (1) Relative water (2) Leaf gas exchange (3) Modulated Chlorophyll a Fluorescence. Different responses in RWC of plant lines to water withdrawal were detected, with transgenic line B5H indicating less water loss after the water withdrawal period. Similar responses to water deficit regarding the leaf gas exchanges were recorded for the three lines. When subjected to water deficit stress situations, higher F v/F m, ΦPSII and qP were detected for the transgenic lines. Under a SWC of 20% where higher values for such parameters were detected with special relevance for the B5H line, indicating a possible higher ability to withstand severe drought stress and to resist to prolonged periods without water than the B1F and WT lines.

Keywords

Trehalose Photosynthesis Water deficit 

Notes

Acknowledgements

Financial support from Fundação para a Ciência e a Tecnologia is acknowledged as grant PRAXIS XXI/BD/21270/99 (FCT / FSE, III Quadro Comunitário de Apoio). Authors would also like to thank Josep Matas Jorba of the Faculty of Biology of the University of Barcelona for technical assistance regarding the leaf gas exchange assay and to P. Fontanet, A. Sanz and E. Saavedra (IBMB, Barcelona, Spain) for Greenhouse work.

References

  1. Almeida AM, Villalobos E, Araújo SS, Leyman B, van Dijk P, Alfaro-Cardoso L, Fevereiro PS, Torné JM, Santos DM (2005) Transformation of tobacco with an Arabidopsis thaliana gene involved in trehalose biosynthesis increases tolerance to several abiotic stresses. Euphytica 146:165–176CrossRefGoogle Scholar
  2. Arrabaça MC (1981) The effect of temperature on photosynthetic and photorespiratory metabolism (PhD thesis), University of London, LondonGoogle Scholar
  3. Bajaj S, Targolli J, Liu LF, Ho TD, Wu R (1999) Transgenic approaches to increase dehydration-stress tolerance in plants. Mol Breed 5:493–503CrossRefGoogle Scholar
  4. Bernfeld P (1955) Methods in enzymology I. Academic Press, New YorkCrossRefGoogle Scholar
  5. Cortina C, Culiáñez-Macià FA (2005) Tomato abiotic stress enhanced tolerance by trehalose biosynthesis. Plant Sci 169:75–82CrossRefGoogle Scholar
  6. Eastmond PJ, Li Y, Graham IA (2003) Is trehalose-6-phosphate a regulator of sugar metabolism in plants? J Exp Bot 54:533–537PubMedCrossRefGoogle Scholar
  7. Elbein AD (1974) The metabolism of α,α-Trehalose. Adv Carbohyd Chem Biochem 30:227–256CrossRefGoogle Scholar
  8. Garg AK, Kim JK, Ranwala AP, Choi YD, Kochian LV, Wu RJ (2002) Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses. Proce Nat Acad Sci USA 99:15898–15903CrossRefGoogle Scholar
  9. Goddijn OJ, Verwoerd TC, Moogd E, Krutwagen RW, de Graaf PT, Poels J, van Dun K, Ponstein AS, Damm B, Pen J (1997) Inhibition of trehalase activity enhances trehalose accumulation in transgenic plants. Plant Physiol 113:181–190PubMedCrossRefGoogle Scholar
  10. Holmström KO, Mäntylä E, Wellin B, Mandal A, Palva ET (1996) Drought tolerance in tobacco. Nature 379:683–684CrossRefGoogle Scholar
  11. Holt NE, Fleming GR, Niyogi KK, (2004) Towards an understanding of the mechanism of nonphotochemical quenching in green plants. Biochemistry 43:8282–8289CrossRefGoogle Scholar
  12. Horsh RB, Fry JF, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT, (1985) Transferring genes into plants. Science 227:1229–1231CrossRefGoogle Scholar
  13. Jang IC, Oh SJ, Seo JS, Choi WB, Song SY, Kim CH, Kim YS, Seo HS, Choi YD, Nahm BH, Kim JK (2003) Expression of a bifunctional fusion of the Escherichia coli genes for Trehalose-6-phosphate synthase and Trehalose-6-phosphate phosphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth. Plant Physiol 131:516–524PubMedCrossRefGoogle Scholar
  14. Leyman B, Van Dijck P, Thevelein J (2001) An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana. Trend Plant Sci 6:510–513CrossRefGoogle Scholar
  15. LI-COR (1990) Li-6200 Technical reference. LI-COR, Lincoln, Nebraska, USAGoogle Scholar
  16. Llorens L, Penuelas J, Estiarte M, Bruna P (2004) Contrasting growth changes in two dominant species of a Mediterranean shrubland submitted to experimental drought and warming. Ann Bot (Lond) 94:843–853CrossRefGoogle Scholar
  17. Marques da Silva J, Arrabaça MC (2004) Photosynthesis in the water-stressed C4 grass Setaria sphacelata is mainly limited by stomata with both rapidly and slowly imposed water deficits. Physiol Plantarum 121:409–420CrossRefGoogle Scholar
  18. Maxwell K, Johnson GN (2000). Chlorophyll fluorescence—a practical guide. J Exp Bot 51:659–668PubMedCrossRefGoogle Scholar
  19. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15:473–497CrossRefGoogle Scholar
  20. Nuccio ML, Rodees D, McNeil S, Hanson AD (1999) Metabolic engineering of plants for osmotic stress resistance. Curr Opin Plant Biol 2:128–134PubMedCrossRefGoogle Scholar
  21. Paul MJ, Driscoll SP, Andralojc PJ, Knight JS, Gray JC, Lawlor DW (2000) Decrease o phosphoribulokinase activity by antisense RNA in transgenic tobacco: definition of the light environment under which phosphoribulokinase is not in large excess. Planta 211:112–119PubMedCrossRefGoogle Scholar
  22. Paul MJ, Pellny T, Goddijn O (2001) Enhancing photosynthesis with sugar signals. Trend Plant Sci 6:197–200CrossRefGoogle Scholar
  23. Pellny TK, Ghannoum O, Conroy JP, Schueppman H, Smeekens S, Androlojc J, Krause KP, Goddijn O, Paul M (2004) Genetic modification of photosynthesis with E. coli genes for trehalose synthesis. Plant Biotechnol J 2:71–82PubMedCrossRefGoogle Scholar
  24. Pena-Rojas K, Aranda X, Fleck I (2004) Stomatal limitation to CO2 assimilation and down-regulation of photosynthesis in Quercus ilex resprouts in response to slowly imposed drought. Tree Physiol 24:813–822PubMedGoogle Scholar
  25. Penna S (2003) Building stress tolerance through over-producing trehalose in transgenic plants. Trend Plant Sci 8:355–357CrossRefGoogle Scholar
  26. Pilon-Smits E, Terry N, Sears T, Kim H, Zayed A, Hwang S, van Dun K, Voogd E, Verwoerd TC, Krutwagen RH, Goddijn OJ (1998) Trehalose-producing transgenic tobacco plants show improved growth performance under drought stress. J Plant Physiol 152:525–532Google Scholar
  27. Pilon-Smits EH, Ebskamp MJ, Paul MJ, Jeuken J, Weisbeek PJ, Smeekens SCM (1995) Improved performance of transgenic fructan-accumulating tobacco under drought stress. Plant Physiol 107:125–130PubMedGoogle Scholar
  28. Roe JH (1934) A colorimetric method for the determination of fructose in blood and urine. J Biol Chem 107:809–818Google Scholar
  29. Romero C, Bellés JM, Vayá JL, Serrano R, Culiañez-Maciá FA (1997) Expression of the yeast trehalose-6-phosphate synthase gene in transgenic tobacco plants: pleiotropic phenotypes include drought tolerance. Planta 201:293–297CrossRefGoogle Scholar
  30. Schluepmann H, van Dijken A, Aghdasi M, Wobbes B, Paul M, Smeekens S (2004) Trehalose mediated growth inhibition of Arabidopsis seedlings is due to trehalose-6-phosphate accumulation. Plant Physiol 135:879–890PubMedCrossRefGoogle Scholar
  31. Schreiber U (1997) Chlorophyll Fluorometer PAM-200 (Teaching PAM) and data acquisition software DA-TEACH, Heinz Walz GmbH, Effeltrich, GermanyGoogle Scholar
  32. Scott P (2000) Resurrection plants and the secret of eternal leaf. Ann Bot 85:159–166CrossRefGoogle Scholar
  33. Sheveleva E, Chamra W, Bohnert HJ, Jensen RG (1997) Increased salt and drought tolerance by d-ononitol production in transgenic Nicotiana tabacum L. Plant Physiol 115:1211–1219PubMedGoogle Scholar
  34. Siedow JN (2001) Feeding ten billion people, three views. Plant Physiol 126:20–22PubMedCrossRefGoogle Scholar
  35. Wingler A (2002) The function of trehalose biosynthesis in plants. Phytochemistry 60:437–440PubMedCrossRefGoogle Scholar
  36. Yan J, He C, Wang J, Mao Z, Halladay SA, Allen RD, Zhang H (2004) Overexpression of the Arabidopsis 14-3-3 protein GF14λ in cotton leads to a stay-green phenotype and improves stress tolerance under moderate drought conditions. Plant Cell Physiol 45:1007–1014PubMedCrossRefGoogle Scholar
  37. Yeo ET, Kwon HB, Han SE, Lee JT, Ryu JC, Byun MO (2000) Genetic engineering of drought resistant potato plants by introduction of the trehalose-6-phosphate synthase (TPS1) gene from Sacharomyces cerevisae. Mol Cells 10:263–268PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • André M. Almeida
    • 1
    • 2
    Email author
  • Anabela B. Silva
    • 3
  • Susana S. Araújo
    • 1
  • Luís A. Cardoso
    • 2
  • Dulce M. Santos
    • 1
  • José M. Torné
    • 4
  • Jorge M. Silva
    • 3
  • Matthew J. Paul
    • 5
  • Pedro S. Fevereiro
    • 1
    • 3
  1. 1.Laboratório de Biotecnologia de Células VegetaisInstituto de Tecnologia Química e Biológica (ITQB)OeirasPortugal
  2. 2.Instituto de Investigação Científica e TropicalLisboaPortugal
  3. 3.Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa (FCUL), LisboaPortugal and Centro de Engenharia Biológica (FCUL)LisboaPortugal
  4. 4.Instituto de Biologia Molecular de Barcelona-CSICBarcelonaSpain
  5. 5.Crop Performance and ImprovementIACR-RothamsteadHarpendenUK

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