SSR marker tightly linked to the Ti locus in Soybean [Glycine max (L.) Merr.]
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Soybean is a major source of protein meal in the world. Soybean kunitz trypsin inhibitor (SKTI) protein is a responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. The primary objective of this research was to identify DNA markers linked to the Ti locus controlling presence and absence of kunitz trypsin inhibitor protein. Two mapping populations were developed. Population 1 was derived from a cross between cultivar Jinpumkong2 (TiTi) and C242 (titi). Population 2 was made from a mating between cultivar Clark (TiTi) and C242. The F1 plants were grown in the greenhouse to produce F2 seeds. Each F2 seed from F1 plants was analyzed electrophoretically to determine the presence of the SKTI protein band. One-thousand RAPD primers, 342 AFLP primer sets, and 35 SSR primers were used to map Ti locus in population 1 and 2. The presence of SKTI protein was dominant to the lack of a SKTI protein and kunitz trypsin inhibit protein band was controlled by a single locus. Twelve DNA markers (4 RAPD, 4 AFLP, and 3 SSR) and Ti locus were found to be genetically linked in population 1 consisted with 94 F2 individual plants. Three SSR markers (Satt409, Satt228, and Satt429) were linked with Ti locus within 10 cM. Satt228 marker was tightly linked with Ti locus. Satt228 marker was tightly linked within 0–3.7 cM of the Ti locus and may be useful in a marker assisted selection program.
KeywordsKunitz trypsin inhibitor Molecular marker Soybean SSR marker Ti locus
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This work was supported by a grant (CG 3122) from Functional Genomics Center (CFGC), 21C Frontier R & D Project, Ministry of Science & Technology, Korea.
- Akkaya MS, Bhagwat AA, Cregan PB (1995) Length polymorphism of simple sequence repeat DNA in soybean. Genetics 132:1131–1139Google Scholar
- Apuya NR, Frazier BL, Keim P, Roth EJ, Lark KG (1988) Restriction fragment length polymorphism as genetic markers in soybean. Theor Appl Genet 75:889–901Google Scholar
- Kim MS, Park MJ, Hwang JG, Jo SH, Ko MS, Chung JI (2004) Identification of molecular markers linked to Ti locus in soybean. Korean J Crop Sci 49(5):419–422Google Scholar
- Kim MS, Cho YJ, Park DJ, Han SJ, Oh JH, Hwang JG, Ko MS, Chung JI (2003) Construction of genetic linkage map for korean soybean genotypes using molecular markers. Korean J Crop Sci 48(4):297–302Google Scholar
- Kosambi DD (1944) The estimation of map distance from recombination values. Ann. Eugen. 12:172–175Google Scholar