Advertisement

Euphytica

, Volume 152, Issue 3, pp 351–360 | Cite as

Insect-mediated seed-set evaluation of 21 soybean lines segregating for male sterility at 10 different loci

  • E. Ortiz-Perez
  • H. T. Horner
  • S. J. Hanlin
  • R. G. Palmer
Article

Abstract

The first requirement to establish a successful hybrid soybean program is the availability of a stable male-sterile, female-fertile system. Male sterility has been an important tool in soybean breeding programs to improve traits such as yield, seed-protein and seed-oil content, and seed size. However, improvement of seed-set per se on male-sterile plants has not been an important breeding objective. The evaluation of the out-crossing potential of the available male-sterile, female-fertile soybean lines is crucial to determine the future of hybrid soybean. The objective of this study was to evaluate seed-set among 21 soybean lines segregating for male sterility at 10 different loci using Megachile rotundata as insect pollinator. Seed-set was evaluated in field conditions in 2001, 2002, and 2003 near Ames, Iowa. Our results indicated significant differences in seed-set among male-sterile lines. The effects of maturity group, pubescence color, the locus and/or the allele, and the genetic background of the lines segregating for male sterility were significant. Differences for seed-set among lines with independent mutational events at the same locus, (i.e. different alleles), also were significant. These results imply that interaction effects with the genetic background of the lines for traits related to fertility/sterility and insect-pollinator attraction and reward are important. Flower color had little effect on seed-set in the evaluated lines. The effect of year was very important on the performance of the lines for seed-set. This suggested that environmental conditions that favor plant–pollinator relationships need to be determined in order to increase insect-mediated cross-pollination to develop an efficient hybrid soybean program.

Keywords

Soybean Male sterility Pollinator Seed-set 

Abbreviations

MG

Maturity group

CV

Coefficient of variation

LSD

Least significant difference

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Notes

Acknowledgments

The authors thank Dairyland Seed Co., Inc, and Verde Seed Co., Inc. for partial support of this project.

References

  1. Bernard RL, Nelson RL, Cremeens CR (1991) USDA soybean genetic collection: isoline collection. Soybean Genet Newsl 18:27–57Google Scholar
  2. Boerma HR, Cooper RL (1978) Increased female fertility associated with the ms1 locus in soybean. Crop Sci 18:344–346CrossRefGoogle Scholar
  3. Boerma HR, Moradshahi A (1975) Pollen movement within and between rows to male-sterile soybean. Crop Sci 15:858–861CrossRefGoogle Scholar
  4. Bradner NR (1969) Hybrid soybeans: fiction or fact? Soybean Digest 29:16–17Google Scholar
  5. Carter TE, Burton JW, Huie EB (1983) Implications of seed-set on ms2ms2 male-sterile plants in Raleigh. Soybean Genet Newsl 10:85–87Google Scholar
  6. Cervantes-Martínez IG, Ortiz-Pérez E, Xu M, Horner HT, Palmer RG (2005) Molecular mapping of the ms2, ms3, and ms9 loci in soybean [Glycine max (L.) Merr.]. Agron Abstr p 257Google Scholar
  7. Chaudhari HH, Davis HW (1977) A new male-sterile strain in Wabash soybeans. J Hered 68:266–267Google Scholar
  8. Delannay X, Palmer RG (1982) Genetics and cytology of the ms4 male-sterile soybean. J Hered 73:219–223Google Scholar
  9. Erickson EH (1975) Variability of floral characteristics influences honey bee visitation to soybean blossoms. Crop Sci 15:767–771CrossRefGoogle Scholar
  10. Erickson EH, Berger GA, Shannon JG, Robbins JM (1978) Honey bee pollination increased soybean yields in the Mississippi delta region of Arkansas and Missouri. J Econ Ent 71:601–603Google Scholar
  11. Fehr WR (1991) Principles of cultivar development. Theory and technique. Macmillan Publishing Company, Ames, IAGoogle Scholar
  12. Fujita R, Ohara M, Okazaki K, Shimamoto Y (1997) The extend of natural cross-pollination in wild soybean (Glycine soja). J Hered 88:124–128Google Scholar
  13. Graybosch RA, Palmer RG (1987) Analysis of a male-sterile character in soybean. J Hered 78:66–70Google Scholar
  14. Graybosch RA, Palmer RG (1988) Male sterility in soybean – an overview. Am J Bot 75:144–156CrossRefGoogle Scholar
  15. Hymowitz T (2004) Speciation and cytogenetics. In: Specht JE, Boerma HR (eds) Soybean: improvement, production and uses. Agron. Monogr.16. ASA, CSSA, and SSSA, 3rd edn. Madison, WI, pp 97–136Google Scholar
  16. Ilarslan H, Horner HT, Palmer RG (1999) Genetics and cytology of a new male-sterile, female-fertile soybean [Glycine max (L.) Merr.] mutant. Crop Sci 39:58–64CrossRefGoogle Scholar
  17. Jin W, Horner HT, Palmer RG (1997) Genetics and cytology of a new genic male-sterile soybean [Glycine max (L.) Merr.]. Sex Plant Reprod 10:13–21CrossRefGoogle Scholar
  18. Kettle WD, Taylor OR (1979) Ecological interaction of honeybees and soybeans. J. Kansas Entomol Soc 52:549Google Scholar
  19. Lewers KS, Palmer RG (1997) Recurrent selection in soybean. Plant Breed Rev 15:275–313Google Scholar
  20. Lewers KS, St Martin SK, Hedges BR, Widrlechner MP, Palmer RG (1996) Hybrid soybean seed production: comparison of three methods. Crop Sci 36: 1560–1567CrossRefGoogle Scholar
  21. Lewers KS, St Martin SK, Hedges BR, Widrlechner MP, Palmer RG (1998) Testcross evaluation of soybean germplasm. Crop Sci 38:1143–1149CrossRefGoogle Scholar
  22. Morrison MJ, Voldeng HD, Guillemette RD (1994) Soybean pubescence color influences seed yield in cool season climates. Agron J 86:796–799CrossRefGoogle Scholar
  23. Ortiz-Perez E, Horner HT, Hanlin SJ, Palmer RG (2006) Evaluation of insect-mediated seed set among soybean lines segregating for male sterility at the ms6 locus. Field Crops Res 97:353–362CrossRefGoogle Scholar
  24. Ortiz-Perez E, Cervantes-Martinez IG, Wiley H, Hanlin SJ, Horner HT, Davis WH, Palmer RG (2004) Phenotypic recurrent selection for increased pollinator attraction to produce hybrid soybean seed. Agron Abstr p 1029Google Scholar
  25. Palmer RG (2000) Genetics of four male-sterile, female-fertile soybean mutants. Crop Sci 40:78–83CrossRefGoogle Scholar
  26. Palmer RG, Winger CL, Albertsen MC (1978) Four independent mutations at the ms1 locus in soybean. Crop Sci 18:727–729CrossRefGoogle Scholar
  27. Palmer RG, Johns CW, Muir PS (1980) Genetics and cytology of the ms3 male-sterile soybean. J Hered 71:343–348Google Scholar
  28. Palmer RG, Albertsen MC, Johns CW (1983) Pollen movement to two male-sterile soybean mutants grown in two locations. J Hered 74:55–57Google Scholar
  29. Palmer RG, Skorupska H (1990) Registration of a male-sterile genetic stock (T295H). Crop Sci 30:244CrossRefGoogle Scholar
  30. Roumet P, Magnier I (1993) Estimation of hybrid seed production and efficient pollen flow using insect pollination of male-sterile soybeans in caged plots. Euphytica 70:61–67CrossRefGoogle Scholar
  31. SAS Institute (2003) SAS/STAT user’s guide, Version 9.0. SAS Inst., Inc., Cary, NCGoogle Scholar
  32. Schoen DJ, Brown AHD (1991) Whole and part flower self-pollination in Glycine clandestina and G. argyrea and the evolution of autogamy. Evolution 45:1651–1664CrossRefGoogle Scholar
  33. Skorupska H, Palmer RG (1989) Genetics and cytology of␣the ms6 male-sterile soybean. J Hered 80:304– 310Google Scholar
  34. Skorupska H, Palmer RG (1990) Additional sterile mutations in soybean Glycine max (L.) Merr. J Hered 81:296–300Google Scholar
  35. Sun H, Zhao LM, Huang M (2000) Cytoplasmic-nuclear male sterile soybean and the method for producing hybrid soybean. The People’s Republic of China Patent No. ZL 97 1 12173.7Google Scholar
  36. Suso MJ, Harder L, Moreno MT, Fouad Maalouf (2005) New strategies for increasing heterozygosity in crops: Vicia faba mating system as study case. Euphytica 143:51–65Google Scholar
  37. Weber CR, Hanson WD (1961) Natural hybridization with and without ionizing radiation in soybean. Crop Sci 1:389–392CrossRefGoogle Scholar
  38. Weber CR, Fehr WR (1967) Effect of hybridization and thermal neutron irradiation on quantitative characters of soybean. Crop Sci 7:78CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • E. Ortiz-Perez
    • 1
  • H. T. Horner
    • 2
  • S. J. Hanlin
    • 3
  • R. G. Palmer
    • 4
  1. 1.Department of AgronomyIowa State UniversityAmesUSA
  2. 2.Department of Genetics, Development and Cell Biology, and Bessey Microscopy FacilityIowa State UniversityAmesUSA
  3. 3.USDA-ARS North Central Regional Plant Introduction StationIowa State UniversityAmesUSA
  4. 4.USDA-ARS CICGR, and Department of AgronomyIowa State UniversityAmesUSA

Personalised recommendations