Applications of pedigree-based genome mapping in wheat and barley breeding programs
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The aim of the pedigree-based genome mapping project is to investigate and develop systems for implementing marker assisted selection to improve the efficiency of selection and increase the rate of genetic gain in breeding programs. Pedigree-based whole genome marker application provides a vehicle for incorporating marker technologies into applied breeding programs by bridging the gap between marker–trait association and marker implementation. We report on the development of protocols for implementation of pedigree-based whole genome marker analysis in breeding programs within the Australian northern winter cereals region. Examples of applications from the Queensland DPI&F wheat and barley breeding programs are provided, commenting on the use of microsatellites and other types of molecular markers for routine genomic analysis, the integration of genotypic, phenotypic and pedigree information for targeted wheat and barley lines, the genomic impacts of strong selection pressure in case study pedigrees, and directions for future pedigree-based marker development and analysis.
KeywordsPedigree mapping Molecular markers Whole genome Hordeum vulgare Triticum aestivum
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This work was funded by the Australian Grains Research and Development Corporation as part of the Australian Winter Cereals Molecular Marker Program.
- Bassam BJ, Caetano-Annollés G (1993) Silver staining of DNA in polyacrylamide gels. Appl Biochem Biotechnol 42:181–188Google Scholar
- Collins H, Logue S, Jefferies S, Barr A (2001) Validation of markers for malt extract, DP, alpha amylase and beta amylase. In: Proc 10th Aust Barley Technical Symp. Canberra, AustraliaGoogle Scholar
- Cooperative Research Centre of Molecular Plant Breeding (2006) http://www.scu.edu.au/research/cpcg/wheat/ index.php. Verified 27/3/06Google Scholar
- Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
- Gupta S, Wielinga C, Li CD, Cakir M, Platz G, Loughman R, Lance R, Appels R (2004) Gene distribution and SSR markers linked with net type net blotch resistance in barley. Proc 9th Int Barley Genet Symp: Session 7Google Scholar
- Jaccoud D, Peng K, Feinstein D, Killian A (2001) Diversity arrays: a solid state technology for sequence information independent genotyping. Nucleic Acid Res 29:4e25Google Scholar
- Lehmensiek A, Eckermann PJ, Verbyla AP, Appels R, Sutherland MW, Daggard GE (2006) Flour yield QTLs in three Australian doubled haploid wheat populations. Aust J Agric Res (in press)Google Scholar
- Liu Z-W, Biyashev RM, Saghai-Maroof M (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theor Appl Genet 93:869–876Google Scholar
- Marquez-Cedillo LA, Hayes PM, Jones BL, Kleinhofs A, Legge WG, Rossnagel BG, Sato K, Ullrich SE, Wesenberg DM (2000) QTL analysis of malting quality in barley based on the doubled-haploid progeny of two elite North American varieties representing different germplasm groups. Theor Appl Genet 101:173–184CrossRefGoogle Scholar
- Nei M (1987) Molecular evolutionary genetics. Colombia University Press, New YorkGoogle Scholar
- Payne RW, Harding SA, Murray DA, Soutar DM, Baird DB, Welham SJ, Kane AF, Gilmour AR, Thompson R, Webster R, Tunnicliffe-Wilson G (2005) GenStat release 8 reference manual, VSN International, OxfordGoogle Scholar
- Rodgers D, Jordan DR, Butler DG (2005) PBMASS: pedigree based marker assisted selection system, version 1.2.1. Queensland Department of Primary Industries and Fisheries, Brisbane, AustraliaGoogle Scholar
- Schmidt AL, Liu CJ, Martin D, Kelly A, McIntyre CL (2004) Molecular markers for selected quality traits in Australian hexaploid bread wheat. Proc 4th Int Crop Science Congress. Brisbane, AustraliaGoogle Scholar
- Schneider A, Roessli D, Excoffier L (2000) Software for population genetics data analysis. Arlequin ver 2000Google Scholar
- Seyfaarth R, Feuillet C, Schachermayr G, Messmer M, Winzeler M, Keller B (2000) Molecular mapping of the adult-plant leaf rust resistance gene Lr13 in wheat (Triticum aestivum L.). J Genet Breed 54:193–198Google Scholar
- Spielmeyer W, Sharp PJ, Rahman R, Lagudah ES (2001) PCR-based markers linked to broad-spectrum stem rust resistance gene Sr2 in wheat. 12th Aust Wheat Breeding Assembly, Mildura, Australia, pp 225–227Google Scholar