More precise map position and origin of a durable non-specific adult plant disease resistance against stripe rust (Puccinia striiformis) in wheat
- 172 Downloads
Recently a major gene determining non-specific adult plant disease resistance against stripe rust (Puccinia striiformis) designated Yrns-B1 was mapped in wheat Triticum aestivum L. by using a cross between ‘Lgst. 79-74’ (resistant) and ‘Winzi’ (susceptible). Linkage to five Gatersleben wheat microsatellite (GWM) markers was discovered, previously mapped on chromosome arm 3BS. In the present study this map was improved by the incorporation of four additional GWM markers. QTL-analysis revealed high LOD values for the resistance at all nine loci, whereas the largest LOD (20.76) was found for the newly mapped marker Xgwm1329.
Microsatellite analysis and resistance tests of a collection of old German/UK wheat varieties, including probable ancestors of ‘Lgst.79-74’ were carried out. A high coincidence of non-specific adult plant disease resistance against stripe rust and the presence of ‘Lgst. 79-74’ allele (117 bp) of the marker Xgwm533 was observed among the varieties tested. Linkage during the inheritance of both the resistance and the 117 bp allele of Xgwm533 was demonstrated. The probable origin of Yrns-B1 is discussed. Carriers of this resistance gene were grown on large areas since more than 100 years. To estimate the capability of Xgwm533 as a diagnostic marker for non-specific adult plant disease resistance against stripe rust, microsatellite analysis and resistance tests of a collection of Russian spring wheat varieties were performed. The 117 bp allele of Xgwm533 was found in about 35% of the Russian cultivars analysed, however, none of them possessed the expected disease resistance. Thus, the utilisation of Xgwm533 as diagnostic marker seems to be restricted to certain genepools.
KeywordsAllele distribution Diagnostic markers Durable non-specific adult plant disease resistance Puccinia striiformis Microsatellites Triticum aestivum Wheat germplasm
Unable to display preview. Download preview PDF.
- Bariana HS, Kailasapillai S, Brown GN, Sharp PJ (1998) Marker assisted identification of Sr2 in the National Cereal Rust Control Program in Australia. In: Slinkard AE (ed) Proceedings of 9th international wheat and genetic symposium, University of Extension Press, University of Saskatchewan, Saskatoon, 3:38–91Google Scholar
- Börner A, Röder MS, Unger O, Meinel A (2001) Non-specific adult-plant disease resistance – genetics and molecular mapping. In: Bedö Z, Lang L (eds) Wheat in a global environment, Kluwer Academic Publishers, pp 317–323Google Scholar
- Flor HH, (1959) Genetic controls and host parasite interactions in rust diseases. In: Holton CS et al (eds) Plant pathology, problems and progress 1908–1958, University of Wisconsin Press, Madison, Wisc., 137–144Google Scholar
- Hillmann P (1910) Die deutsche landwirtschaftliche Pflanzenzucht. Arbeiten der DLG, Heft 168:603 ppGoogle Scholar
- Lupton FGH (1992) Changes in varietal distribution of cereals in central and western Europe. Agro-Ecological Atlas of Cereal Growing in Europe, vol. IV, Pudoc WageningenGoogle Scholar
- McIntosh RA, Luig NH, Johnson R, Hare RA (1981) Cytogenetical studies in wheat. XI. Sr9g for reaction to Puccinia graminis tritici. Z Pflanzenzüchtg 87:274–289Google Scholar
- McIntosh RA, Yamazaki Y, Devos KM, Dubcovsky J, Rogers J, Appels R (2003) Catalogue of gene symbols for wheat http://www.grs.nig.ac.jp/wheat/komugi/genes/
- Meinel A, Unger O (1998) Breeding aspects of partial resistance to airborne pathogens in wheat. Czech J Genet Plant Breed 34:103–106Google Scholar
- Rimpau W (1883) Züchtung auf dem Gebiete der landwirthschaftlichen Kulturpflanzen. Mentzel & von Lengerke’s Landwirthschaftlicher Kalender II, pp 33–92Google Scholar
- Rimpau W (1887) Produkten-Ausstellung von W. Rimpau. Archiv Prof. Rimpau, BerlinGoogle Scholar
- Rimpau W (1891) Kreuzungsprodukte Landwirthschaftlicher Kulturpflanzen, Parey, 39 ppGoogle Scholar
- Römer Th, Fuchs WH, Isenbeck K (1938) Die Züchtung resistenter Rassen der Kulturpflanzen, PareyGoogle Scholar
- Singh RP, William HM, Huerta-Espino J, Grospy M (2003) Identification and mapping of gene Yr31 for resistance to stripe rust in Triticum aestivum cultivar Pastor. In: Proceedings of 10th international wheat genet symposium, Paestium, Italy, pp 411–413Google Scholar
- Stubbs RW (1985) Stripe rust. In Roelfs AP, Bushnell WR (Eds.) The cereal rusts II. Academic Press, Orlando, Florida, USA, pp 61–101Google Scholar
- Worland AJ, Korzun V, Röder MS, Ganal MW, Law CN (1998) Genetic analysis of the dwarfing gene Rht8 in wheat. Part II. The distribution and adaptive significance of allelic variants at the Rht8 locus of wheat as revealed by microsatellite screening. Theor Appl Genet 96:1110–1120CrossRefGoogle Scholar