Advertisement

Euphytica

, Volume 153, Issue 1–2, pp 1–10 | Cite as

More precise map position and origin of a durable non-specific adult plant disease resistance against stripe rust (Puccinia striiformis) in wheat

  • E. K. Khlestkina
  • M. S. Röder
  • O. Unger
  • A. Meinel
  • A. Börner
Article

Abstract

Recently a major gene determining non-specific adult plant disease resistance against stripe rust (Puccinia striiformis) designated Yrns-B1 was mapped in wheat Triticum aestivum L. by using a cross between ‘Lgst. 79-74’ (resistant) and ‘Winzi’ (susceptible). Linkage to five Gatersleben wheat microsatellite (GWM) markers was discovered, previously mapped on chromosome arm 3BS. In the present study this map was improved by the incorporation of four additional GWM markers. QTL-analysis revealed high LOD values for the resistance at all nine loci, whereas the largest LOD (20.76) was found for the newly mapped marker Xgwm1329.

Microsatellite analysis and resistance tests of a collection of old German/UK wheat varieties, including probable ancestors of ‘Lgst.79-74’ were carried out. A high coincidence of non-specific adult plant disease resistance against stripe rust and the presence of ‘Lgst. 79-74’ allele (117 bp) of the marker Xgwm533 was observed among the varieties tested. Linkage during the inheritance of both the resistance and the 117 bp allele of Xgwm533 was demonstrated. The probable origin of Yrns-B1 is discussed. Carriers of this resistance gene were grown on large areas since more than 100 years. To estimate the capability of Xgwm533 as a diagnostic marker for non-specific adult plant disease resistance against stripe rust, microsatellite analysis and resistance tests of a collection of Russian spring wheat varieties were performed. The 117 bp allele of Xgwm533 was found in about 35% of the Russian cultivars analysed, however, none of them possessed the expected disease resistance. Thus, the utilisation of Xgwm533 as diagnostic marker seems to be restricted to certain genepools.

Keywords

Allele distribution Diagnostic markers Durable non-specific adult plant disease resistance Puccinia striiformis Microsatellites Triticum aestivum Wheat germplasm 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bariana HS, McIntosh RA (1993) Cytogenetic studies in wheat. XV. Location of rust resistance genes in VPM1 and their genetic linkage with other disease resitance genes in chromosome 2A. Genome 36:476–482PubMedGoogle Scholar
  2. Bariana HS, McIntosh RA (1995) Genetics of adult plant stripe rust resistance in four Australian wheats and the French cultivar ‘Hybride-de-Bersee’. Plant Breed 114:485–491CrossRefGoogle Scholar
  3. Bariana HS, Hayden MJ, Ahmed NU, Bell JA, Sharp PJ, McIntosh RA (2001) Mapping of durable adult plant and seedling resistances to stripe rust and stem rust diseases in wheat. Aust J Agric Res 52:1247–1255CrossRefGoogle Scholar
  4. Bariana HS, Kailasapillai S, Brown GN, Sharp PJ (1998) Marker assisted identification of Sr2 in the National Cereal Rust Control Program in Australia. In: Slinkard AE (ed) Proceedings of 9th international wheat and genetic symposium, University of Extension Press, University of Saskatchewan, Saskatoon, 3:38–91Google Scholar
  5. Börner AM, Röder S, Unger O, Meinel A (2000) The detection and molecular mapping of a major gene for non-specific adult-plant disease resistance against stripe rust (Puccinia striiformis) in wheat. Theor Appl Genet 100:1095–1099CrossRefGoogle Scholar
  6. Börner A, Röder MS, Unger O, Meinel A (2001) Non-specific adult-plant disease resistance – genetics and molecular mapping. In: Bedö Z, Lang L (eds) Wheat in a global environment, Kluwer Academic Publishers, pp 317–323Google Scholar
  7. Börner A, Schumann E, Fürste A, Cöster H, Leithold B, Röder MS, Weber WE (2002) Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet 105:921–936PubMedCrossRefGoogle Scholar
  8. Boukhatem N, Baret PV, Mingeot D, Jacquemin JM (2002) Quantitative trait loci for resistance against yellow rust in two wheat-derived recombinant inbred line populations. Theor Appl Genet 104:111–118PubMedCrossRefGoogle Scholar
  9. Flor HH, (1959) Genetic controls and host parasite interactions in rust diseases. In: Holton CS et al (eds) Plant pathology, problems and progress 1908–1958, University of Wisconsin Press, Madison, Wisc., 137–144Google Scholar
  10. Gupta PK, Varshney RK, Sharma PC, Ramesh B (1999) Molecular markers and their applications in wheat breeding. Plant Breed 118:369–390CrossRefGoogle Scholar
  11. Hillmann P (1910) Die deutsche landwirtschaftliche Pflanzenzucht. Arbeiten der DLG, Heft 168:603 ppGoogle Scholar
  12. Hsam SLK, Mohler V, Hartl L, Wenzel G, Zeller FJ (2000) Mapping of powdery mildew and leaf rust resistance genes on the wheat-rye translocated chromosome T1BL.1RS using molecular and biochemical markers. Plant Breed 119:87–89CrossRefGoogle Scholar
  13. Imtiaz M, Ahmad M, Cromey MG, Griffin WB Hampton JG (2004) Detection of molecular markers linked to the durable adult plant stripe rust resistance gene Yr18 in bread wheat (Triticum aestivum L.). Plant Breed 123:401–404CrossRefGoogle Scholar
  14. Islam MR, Shepherd KW, Mayo GME (1989) Recombination among genes at the L group in flax conferring resistant to rust. Theor Appl Genet 77:540–546CrossRefGoogle Scholar
  15. Khlestkina EK, Röder MS, Efremova TT, Börner A, Shumny VK (2004) The genetic diversity of old and modern Siberian varieties of common spring wheat determined by microsatellite markers. Plant Breed 123:122–127CrossRefGoogle Scholar
  16. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg I (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181PubMedCrossRefGoogle Scholar
  17. Lupton FGH (1992) Changes in varietal distribution of cereals in central and western Europe. Agro-Ecological Atlas of Cereal Growing in Europe, vol. IV, Pudoc WageningenGoogle Scholar
  18. Mago R, Spielmeyer W,Lawrence GJ, Lagudah ES, Ellis JG, Pryor A (2002) Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theor Appl Genet 104:1317–1324PubMedCrossRefGoogle Scholar
  19. McIntosh RA, Luig NH, Johnson R, Hare RA (1981) Cytogenetical studies in wheat. XI. Sr9g for reaction to Puccinia graminis tritici. Z Pflanzenzüchtg 87:274–289Google Scholar
  20. McIntosh RA, Yamazaki Y, Devos KM, Dubcovsky J, Rogers J, Appels R (2003) Catalogue of gene symbols for wheat http://www.grs.nig.ac.jp/wheat/komugi/genes/
  21. Meinel A (2003) An early scientific approach to heredity by the plant breeder Wilhelm Rimpau (1842–1903). Plant Breed 122:195–198CrossRefGoogle Scholar
  22. Meinel A, Unger O (1998) Breeding aspects of partial resistance to airborne pathogens in wheat. Czech J Genet Plant Breed 34:103–106Google Scholar
  23. Nelson JC (1997) QGENE: software for mapmaker-based genomic analysis and breeding. Mol Breed 3:239–245CrossRefGoogle Scholar
  24. Nelson JC, Singh RP, Autrique JE, Sorrells ME (1997) Mapping genes conferring and suspecting leaf rust resistance in wheat. Crop Sci 37:1928–1935CrossRefGoogle Scholar
  25. Plaschke J, Ganal MW, Röder MS (1995) Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor Appl Genet 91:1001–1007CrossRefGoogle Scholar
  26. Rimpau W (1883) Züchtung auf dem Gebiete der landwirthschaftlichen Kulturpflanzen. Mentzel & von Lengerke’s Landwirthschaftlicher Kalender II, pp 33–92Google Scholar
  27. Rimpau W (1887) Produkten-Ausstellung von W. Rimpau. Archiv Prof. Rimpau, BerlinGoogle Scholar
  28. Rimpau W (1891) Kreuzungsprodukte Landwirthschaftlicher Kulturpflanzen, Parey, 39 ppGoogle Scholar
  29. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  30. Römer Th, Fuchs WH, Isenbeck K (1938) Die Züchtung resistenter Rassen der Kulturpflanzen, PareyGoogle Scholar
  31. Singh RP, Nelson JC, Sorrells ME (2000) Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Sci 40:1148–1155CrossRefGoogle Scholar
  32. Singh RP, William HM, Huerta-Espino J, Grospy M (2003) Identification and mapping of gene Yr31 for resistance to stripe rust in Triticum aestivum cultivar Pastor. In: Proceedings of 10th international wheat genet symposium, Paestium, Italy, pp 411–413Google Scholar
  33. Spielmeyer W, Sharp PJ, Lagudah ES (2003) Identification and validation of markers linked to broad-spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.) Crop Sci 43:333–336CrossRefGoogle Scholar
  34. Stubbs RW (1985) Stripe rust. In Roelfs AP, Bushnell WR (Eds.) The cereal rusts II. Academic Press, Orlando, Florida, USA, pp 61–101Google Scholar
  35. William M, Singh RP, Huerta-Espino J, Ortiz Islas S, Hoisington D (2003) Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat. Phytopathology 93:153–159PubMedGoogle Scholar
  36. Worland AJ, Korzun V, Röder MS, Ganal MW, Law CN (1998) Genetic analysis of the dwarfing gene Rht8 in wheat. Part II. The distribution and adaptive significance of allelic variants at the Rht8 locus of wheat as revealed by microsatellite screening. Theor Appl Genet 96:1110–1120CrossRefGoogle Scholar
  37. Zhou WC, Kolb FL, Bai GH, Domier LL, Boze LK, Smith NJ (2003) Validation of a major QTL for scab resistance with SSR markers and use of marker-assisted selection in wheat. Plant Breed 122:40–46CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • E. K. Khlestkina
    • 1
    • 2
  • M. S. Röder
    • 1
  • O. Unger
    • 3
  • A. Meinel
    • 3
  • A. Börner
    • 1
  1. 1.Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)GaterslebenGermany
  2. 2.Institute of Cytology and Genetics Siberian Branch of the Russian Academy of SciencesNovosibirskRussia
  3. 3.NORDSAAT Saatzuchtgesellschaft mbHBöhnshausenGermany

Personalised recommendations