, Volume 152, Issue 1, pp 67–73 | Cite as

Modifier QTL for fatty acid composition in soybean oil

  • D. R. Panthee
  • V. R. Pantalone
  • A. M. Saxton
Original Article


Soybean [Glycine max (L.) Merr.] is the principal oilseed crop in the world. Soybean oil has various industrial and food applications. The quality of soybean oil is determined by its fatty acid composition. Palmitic, stearic, oleic, linoleic and linolenic are the predominant fatty acids in soybean oil. The objective of this study was to determine the associations of simple sequence repeat (SSR) molecular markers with minor differences in fatty acids in soybean oil thereby detecting modifier quantitative trait loci (QTL) which could further improve soybean oil quality. To achieve this objective, 101 F6-derived recombinant inbred lines (RIL) from a population whose parents did not contain major mutant fatty acid alleles were developed from a cross of N87-984-16 × TN93-99. Fatty acids were determined by gas chromatography. Heritability estimates on an entry mean basis for fatty acids ranged from 65.8 to 77.3% for palmitic and linoleic acids, respectively. Molecular marker Satt537 located on molecular linkage group (MLG) D1b was associated with palmitic acid and Satt168 and Satt249 located on MLG B2 and J, respectively were associated with stearic acid. Molecular markers Satt185 or Satt268 (which are within 0.6 cM of each other) located on MLG E were consistently associated with oleic and linoleic acid, and Satt263 and Satt235 located on MLG E and G, respectively were associated with linolenic acid. The lack of markers associated with multiple fatty acids suggests the possibility of independently changing fatty acid levels to achieve a desirable composition, except for regions common to all saturated fatty acids. Phenotypic variation explained by the fatty acids modifier QTL ranged from 10 to 22.5%. These modifier QTL may be useful in making minor improvements to further enhance the quality of soybean oil.


Glycine max Molecular markers SSR 


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  1. Brummer EC, Graef GL, Orf J, Wilcox JR, Shoemaker RC (1997) Mapping QTL for seed protein and oil content in eight soybean populations. Crop Sci 37:370–378CrossRefGoogle Scholar
  2. Burton JW, Carter TE, Wilson RF (1999) Registration of ‘Prolina’ soybean. Crop Sci 39:294–295CrossRefGoogle Scholar
  3. Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971PubMedGoogle Scholar
  4. Cregan PB, Jarvik T, Bush AL, Shoemaker RC, Lark KG, Kahler AL, Kaya N, VanToai TT, Lohnes DG, Chung J (1999) An integrated genetic linkage map of the soybean genome. Crop Sci 39:1464–1490CrossRefGoogle Scholar
  5. Csanadi G, Vollmann J, Stift G, Lelley T (2001) Seed quality QTLs identified in a molecular map of early maturing soybean. Theor Appl Genet 103:912–919CrossRefGoogle Scholar
  6. Diers BW, Shoemaker RC (1992) Restriction fragment length polymorphism analysis of soybean fatty acid content. J Am Oil Chem Soc 69:1242–1244Google Scholar
  7. Hyten DL, Pantalone VR, Sams CE, Saxton AM, Landau-Ellis D, Stefaniak TR, Schmidt ME (2004a) Seed quality QTL in a prominent soybean population. Theor Appl Genet 109:552–561CrossRefGoogle Scholar
  8. Hyten DL, Pantalone VR, Saxton AM, Schmidt ME, Sams CE (2004b) Molecular mapping & identification of soybean fatty acid modifier quantitative trait loci. J Am Oil Chem Soc 81:1115–1118Google Scholar
  9. Li Z, Wilson RF, Rayford WE, Boerma HR (2002) Molecular mapping genes conditioning reduced palmitic acid content in N87-2122-4 soybean. Crop Sci 42:373–378CrossRefGoogle Scholar
  10. Lincoln SE, Daly MJ, Lander ES (1993) Constructing genetic linkage maps with MAPMAKER Version 3.: A tutorial & reference manual. Release 3rd edn. Whitehead Institute for Biomedical Research, Cambridge, MAGoogle Scholar
  11. Mansur LM, Orf JH, Chase K, Jarvik T, Cregan PB, Lark KG (1996) Genetic mapping of agronomic traits using recombinant inbred lines of soybean. Crop Sci 36:1327–1336CrossRefGoogle Scholar
  12. Nyquist WE (1991) Estimation of heritability & prediction of selection response in plant populations. Crit Rev Plant Sci 10:235–322CrossRefGoogle Scholar
  13. Pantalone V, Walker D, Dewey R, Rajcan I. (2004) DNA marker-assisted selection for improvement of soybean oil concentration & quality, p. 283–311, In:Wilson R, et al., (eds.) Legume crop genomics. AOCS Press, Champaign, ILGoogle Scholar
  14. Pantalone VR, Allen FL, Landau-Ellis D (2003) Registration of TN93-99 Soybean germplasm. Crop Sci 43:1137CrossRefGoogle Scholar
  15. Qiu BX, Rao Arelli AP, Sleper DA. (1997). RFLP markers associated with soybean oil & protein concentration. Soyb Genet Newsl 24:206–207Google Scholar
  16. SAS Institute Inc (2002) SAS guide for personal computers. 9th ed. SAS Institute, Cary, NCGoogle Scholar
  17. Song QJ, Marek LF, Shoemaker RC, Lark KG, Concibido VC, Delannay X, Specht JE, Cregan PB (2004) A new integrated genetic linkage map of the soybean. Theor Appl Genet 109:122–128PubMedCrossRefGoogle Scholar
  18. Soytech Inc (2004) Oilseed statistics. Soytech Inc., Bar Harbor, MEGoogle Scholar
  19. Spencer MM, Pantalone VR, Meyer EJ, Landau Ellis D, Hyten Jr. DL (2003) Mapping the Fas locus controlling stearic acid content in soybean. Theor Appl Genet 106:615–619PubMedGoogle Scholar
  20. Wang S, Basten CJ, Zeng ZB (2003) Windows QTL Cartographer. Release V2.0. North Carolina State University, Raleigh, NCGoogle Scholar
  21. Wilson R, Burton J, Pantalone V, Dewey R (2002) New gene combinations governing saturated & unsaturated fatty acid composition in soybean, In: Kuo T, Gardner H (eds.). Lipid Biotechnology. Marcel Dekker, Inc., Basel, New York, pp. 95–113Google Scholar
  22. Wilson RF (1996) Current research on oilseeds Institute of Food Technologists 1996 Symposium: Identify Preserved Oils. Institute of Food Technologists, New Orleans, LAGoogle Scholar
  23. Wilson RF (2004) Seed composition In: Boerma HR, Specht JE (eds.). Soybeans: Improvement, Production & Uses, 3rd ed. ASA, CSSA, SSA, Madison, WIGoogle Scholar

Copyright information

© Springer Science + Business Media B.V. 2006

Authors and Affiliations

  • D. R. Panthee
    • 1
  • V. R. Pantalone
    • 1
  • A. M. Saxton
    • 2
  1. 1.Department of Plant SciencesUniversity of TennesseeKnoxvilleUSA
  2. 2.Department of Animal ScienceUniversity of TennesseeKnoxvilleUSA

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