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Euphytica

, Volume 151, Issue 3, pp 311–319 | Cite as

Genetic Combining Ability of Petal Shape in Garden Pansy (Viola × wittrockiana Gams) based on Image Analysis

  • Y. Yoshioka
  • H. Iwata
  • N. Hase
  • S. Matsuura
  • R. Ohsawa
  • S. NinomiyaEmail author
Original Article

Abstract

Flower appearance is an important target for improvement in garden pansy (Viola × wittrockiana Gams). Flowers of this species consist of three petal types: inferior, lateral, and superior. By means of principal-components (PC) analysis of elliptic Fourier descriptors (EFDs), we estimated the general and specific combining abilities (GCA and SCA) of the floral characteristics of F1 progeny derived from diallel crosses of four inbred lines. The greatest variation in petal shape was explained by the aspect ratio (the first PC) in each petal type. The second or third PC of each petal type was associated with the curvatures of the various parts of the petal. The highly significant GCA effects indicate the importance of additive genetic variance in the transmission of parental petal characteristics to the progeny. The fact that the SCA mean squares were not significant for aspect ratio and petal area indicates that these characteristics of a single-cross progeny can be sufficiently predicted on the basis of GCA. Significant SCA effects were observed in the curvatures of the distal and proximal parts of lateral and superior petals. Correlation analyses indicated several associations between the shape elements of lateral and superior petals, suggesting that genes for these shape elements may be associated, linked, or pleiotropic in the parents used in this study. We successfully demonstrated the use of EFD–PCA to evaluate the petal shape of garden pansy, and of analyzing combining ability and correlations based on the PC scores of EFDs.

Keywords

Combining ability Elliptic Fourier descriptor Floral variation Heritability Principal component analysis 

Abbreviations

EFD

Elliptic Fourier descriptor

GCA

General combining ability

PA

Petal area

PC

Principal component

PCA

Principal-components analysis

SCA

Specific combining ability

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Copyright information

© Springer Science + Business Media B.V. 2006

Authors and Affiliations

  • Y. Yoshioka
    • 1
  • H. Iwata
    • 2
  • N. Hase
    • 3
  • S. Matsuura
    • 3
  • R. Ohsawa
    • 1
  • S. Ninomiya
    • 4
    • 5
    Email author
  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan
  2. 2.Department of Information Science and TechnologyNational Agricultural Research CenterTsukubaJapan
  3. 3.Tohoku Seed CompanyUtsunomiyaJapan
  4. 4.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan
  5. 5.Department of Information Science and TechnologyNational Agricultural Research CenterTsukubaJapan

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