Euphytica

, Volume 143, Issue 3, pp 239–242 | Cite as

Red grain colour gene (R) of wheat is a Myb-type transcription factor

Article

Abstract

Red pigment of wheat grain was reported to be polyphenol compounds, phlobaphene or proanthocyanidin, which were synthesized through the flavonoid biosynthesis pathway. We examined the expression of four genes in the flavonoid pathway in developing wheat grains: chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), and dihydroflavonol 4-reductase (DFR). The expression of these genes in the white-grained wheat lines was reduced, compared with that in the red-grained wheats. These results suggest that the R genes for grain colour, located on the long arms of chromosomes 3A, 3B and 3D, are transcriptional activators of the flavonoid synthesis genes. We identified three Myb-type transcription factors (Tamyb10-A1 on 3A, Tamyb10-B1 on 3B and Tamyb10-D1 on 3D), which were also located in the same region as the R loci, and were expressed predominantly in developing grains. In wheat lines with the recessive allele r3 (3B) Tamyb10-B1 had a deletion which caused a frame-shift in its open reading frame. Tamyb10-A1, and -D1 were not expressed in wheats with r2 (3A) and r1 (3D).

Keywords

dormancy flavonoid MYB pigment transcription factor 

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References

  1. Athma, P., E. Grotewold & T. Peterson, 1992. Insertional mutagenesis of the maize P gene by intragenic transposition of Ac. Genetics 131: 199–209.PubMedGoogle Scholar
  2. Flintham, J.E., 2000. Different genetic components control coat-imposed and embryo-imposed dormancy in wheat. Seed Sci Res 10: 43–50.Google Scholar
  3. Himi, E., D.J. Mares, A. Yanagisawa & K. Noda, 2002. Effect of grain colour gene (R) on grain dormancy and sensitivity of the embryo to abscisic acid (ABA) in wheat. J Exp Bot 53: 1569–1574.CrossRefPubMedGoogle Scholar
  4. Himi, E., A. Nisar & Kaz. Noda, 2005. Colour genes (R and Rc) for grain and coleoptile upregulate flavonoid biosynthesis genes in wheat. Genome (in print).Google Scholar
  5. Koval, S.F., 1997. The catalog of near-isogenic lines of Novosibirskaya-67 common wheat and principles of their use in experiments. Russ J Genet 33: 995–1000.Google Scholar
  6. Martin, C., A. Prescott, S. Mackay, J. Bartlett & E. Vrijlandt, 1991. Control of anthocyanin biosynthesis in flowers of Antirrhinum majus. Plant J 1: 37–49.PubMedGoogle Scholar
  7. McIntosh, R.A., G.E. Hart, K.M. Devos, M.D. Gale & W.J. Rogers, 1998. Catalogue of gene symbols for wheat. In: Proceedings of the Ninth International Wheat Genetics Symposium, Vol. 5. University of Saskatchewan Extension Press, Canada.Google Scholar
  8. Miyamoto, T. & E.H. Everson, 1958. Biochemical and physiological studies of wheat seed pigmentation. Agron J 50: 733–734.Google Scholar
  9. Nesi, N., C. Jond, I. Debeaujon, M. Caboche & L. Lepiniec, 2001. The Arabidopsis TT2 gene encodes an R2R3 MYB domain protein that acts as a key determinant for proanthocyanidin accumulation in developing seed. Plant Cell 13: 2099–2114.CrossRefPubMedGoogle Scholar
  10. Radicella, J.P., D. Turks & V.L. Chandler, 1991. Cloning and nucleotide sequence of a cDNA encoding B-Peru, a regulatory protein of the anthocyanin pathway in maize. Plant Mol Biol 17: 127–130.CrossRefPubMedGoogle Scholar
  11. Vom Endt, D., J. Kijne & J. Memelink, 2002. Transcription factors controlling plant secondary metabolism: What regulates the regulators? Phytochemistry 61: 107–114.CrossRefPubMedGoogle Scholar
  12. Warner, R.L., D.A. Kudrna, S.C. Spaeth & S.S. Jones, 2000. Dormancy in wheat-grain mutants of Chinese Spring wheat (Triticum aestivum L.). Seed Sci Res 10: 51–60.Google Scholar
  13. Winkel-Shirley, B., 2001. Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol 126: 485–493.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  1. 1.Research Institute for BioresourcesOkayama UniversityKurashikiJapan

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