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Euphytica

, Volume 144, Issue 3, pp 291–299 | Cite as

Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean

  • Gerardine Mukeshimana
  • Astrid Pañeda
  • Cristina Rodríguez-Suárez
  • Juan José Ferreira
  • Ramón Giraldez
  • James D. Kelly
Article

Summary

Necrotic strains of bean common mosaic potyviruses are becoming increasingly problematic in bean growing areas of Africa and Europe. Pyramiding epistatic resistance genes provides the most effective long-term strategy for disease control against all known strains of the virus. Indirect selection using tightly linked markers should facilitate the breeding of desired epistatic resistance gene combinations. In common bean, the most effective strategy for broad spectrum control of the bean common mosaic potyviruses is to combine I and bc-3 genes. We describe the use of near-isogenic lines and segregating populations from different gene pools combined with bulked segregant analysis to identify markers tightly linked with the recessive bc-3 gene that conditions resistance to all strains of bean common mosaic necrosis virus. We identified a RAPD marker, OG6595, linked at 3.7 cM from the bc-3, and the marker was used to confirm the location of bc-3 gene on bean linkage group B6. A codominant AFLP marker, E ACA M CGG -169/172 was identified and linked at 3.5 cM from the bc-3 and the AFLP and OG6595 markers flanked the bc-3 gene. The AFLP marker was converted to the STS marker SE ACA M CGG -134/137 which showed co-segregation with the original AFLP marker. The 134 bp fragment associated with resistance was linked with the bc-3 gene present in a diverse group of bean genotypes except in two kidney bean lines. The OG6595 marker mapped on B6 supported independence of bc-3 from the I gene located on B2, which provides the opportunity to readily combine both genes in a single bean cultivar for broad spectrum resistance to bean common mosaic potyviruses.

Key words

AFLP bean common mosaic virus (BCMV) bean common mosaic necrosis virus (BCMNV) gene pyramiding Phaseolus vulgaris, RAPD STS markers 

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References

  1. Afanador, L.K., S.D. Haley & J.D. Kelly, 1993. Adoption of mini-prep DNA extraction method for RAPD marker analysis in common bean (Phaseolus vulgaris). Annu Rep Bean Improv Coop 36: 10–11.Google Scholar
  2. Ali, M.A., 1950. Genetics of resistance to the common bean mosaic virus in the bean (Phaseolus vulgaris L.). Phytopathology 40: 69–79.Google Scholar
  3. Beebe, S., P.W. Skroch, J. Tohme, M.C. Duque, F. Pedroza & J. Nienhuis, 2000. Structure of genetic diversity among common bean landraces of Middle American origin based on correspondence analysis of RAPD. Crop Sci 40: 264–273.Google Scholar
  4. Beebe, S., J. Rengifo, E. Gaitan, M.C. Duque & J. Tohme, 2001. Diversity and origin of Andean landraces of common bean. Crop Sci 41: 854–862.Google Scholar
  5. Coyne, D.P., J.R. Steadman, G. Godoy-Lutz, R. Gilbertson, E. Arnaud-Santana, J.S. Beaver & J.R. Myers, 2003. Contribution of the Bean/Cowpea CRSP to management of bean disease. Field Crops Res 82: 87–102.Google Scholar
  6. Drijfhout, E., 1978. Genetic interaction between Phaseolus vulgaris and bean common mosaic virus with implications for strain identification and breeding resistance. Agric Res Rep 872: 1–98. Centre for Agriculture Publishing and documentation, Wageningen, Netherlands.Google Scholar
  7. Drijfhout, E., 1991. Bean common mosaic. In: R. Hall (Ed.), Compendium of Bean Diseases, pp. 37–39. APS Press, St. Paul, MN.Google Scholar
  8. Flores-Estévez, N., J.A. Acosta-Gallegos & L. Silva-Rosales, 2003. Bean common mosaic virus and bean common mosaic necrosis virus in Mexico. Plant Dis 87: 21–25.Google Scholar
  9. Fraser, R.S., 1992. The genetics of plant-virus interactions: Implications for plant breeding. Euphytica 63: 175–1992.Google Scholar
  10. Freyre, R., P.W. Skroch, V. Geffroy, A.F. Adam-Blondon, A. Shirmohamadali, W.C. Johnson, V. Llaca, R.O. Nodari, P.A. Pereira, S.M. Tsai, J. Tohme, M. Dron, J. Nienhuis, C.E. Vallejos & P. Gepts, 1998. Towards an integrated linkage map of common bean. 4. Development of a core linkage map and alignment of RFLP maps. Theor Appl Genet 97: 847–856.Google Scholar
  11. Gepts, P., 1999. Development of an integrated linkage map. In: S.P. Singh (Ed.), Development in plant breeding. Common bean improvement in the twenty-first century, pp. 53–91. Kluwer Academic Publisher, Dordrecht, The Netherlands.Google Scholar
  12. Haley, S.D., P.N. Miklas, J.R. Stavely, J. Byrum & J.D. Kelly, 1993. Identification of RAPD markers linked to a major rust resistance gene block in common bean. Theor Appl Genet 86: 505–512.Google Scholar
  13. Haley, S.D., L. Afanador & J.D. Kelly, 1994a. Selection for monogenic pest traits with coupling and repulsion phase RAPD markers. Crop Sci 34: 1061–1066.Google Scholar
  14. Haley, S.D., L. Afanador & J.D. Kelly, 1994b. Identification and application of a random amplified polymorphic DNA marker for the I gene (potyvirus resistance) in common bean. Phytopathology 84: 157–160.Google Scholar
  15. Haley, S.D., L. Afanador & J.D. Kelly, 1994c. Heterogeneous inbred populations are useful as sources of near-isogenic lines for RAPD marker localization. Theor Appl Genet 88: 337–342.Google Scholar
  16. Harrison, B.D., 2002. Virus variation in relation to resistance breaking in plants. Euphytica 124: 181–192.Google Scholar
  17. Hart, L.P. & A.W. Saettler, 1981. Bean common mosaic virus. Michigan State University, Extension Bulletin E-1561.Google Scholar
  18. Hazen, S.P., P. Leroy & R.W. Ward, 2002. AFLP in Triticum aestivum L.: Pattern of genetic diversity and genome distribution. Euphytica 125: 89–102.Google Scholar
  19. ISAR, Bean program, 2001. Activity review, August 2001. p 1–8. http://www.isar.cgiar.org/Isarprog/Bean.htm
  20. Johansen, I.E., O.S. Lund, C.K. Hjulsager & J. Laursen, 2001. Recessive resistance in Pisum sativum and potyvirus pathotype resolved in gene for cistron correspondence between host and virus. J Virol 75: 6609–6614.Google Scholar
  21. Johnson, W.C., P. Guzman, D. Mandala, A.B.C. Mkandawire, S. Temple, R.L. Gilbertson & P. Gepts, 1997. Molecular tagging of bc-3 gene for introgression into Andean common bean. Crop Sci 37: 248–254.Google Scholar
  22. Kelly, J.D., 1997. A review of varietal response to bean common potyvirus in Phaseolus vulgaris. Plant Varieties Seeds 10: 1–6.Google Scholar
  23. Kelly, J.D., A.W. Saettler & M.W. Adams, 1983. New necrotic strain of bean common mosaic virus in Michigan. Annu Rep Bean Improv Coop 26: 49–50.Google Scholar
  24. Kelly, J.D., G.L. Hosfield, G.V. Varner, M.A. Uebersax, S.D. Haley & J. Taylor, 1994. Registration of ‘Raven’ black bean. Crop Sci 34: 1406–1407.Google Scholar
  25. Kelly, J.D., L. Afanador & S.D. Haley, 1995. Pyramiding genes for resistance to bean common mosaic virus. Euphytica 82: 207–212.Google Scholar
  26. Kelly, J.D., P. Gepts, P.N. Miklas & D.P. Coyne, 2003.Tagging and mapping of genes and QTL and molecular-marker assisted selection for traits of economic importance in bean and cowpea. Field Crops Res 82: 135–154.Google Scholar
  27. Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincoln & L. Newburg, 1987. MAPMAKER: An interactive computing package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–181.Google Scholar
  28. McKern, N.M., G.I. Mink, O.W. Barnett, A. Mishra, L.A. Whittaker, M.J. Silbernagel, C.W. Ward & D.D. Shukla, 1992. Isolates of bean common mosaic virus comprising two distinct potyviruses. Phytopathology 82: 923–928.Google Scholar
  29. Melotto, M., L. Afanador & J.D. Kelly, 1996. Development of a SCAR marker linked to the I gene in common bean. Genome 39: 1216–1219.Google Scholar
  30. Michelmore, R.W., I. Paran & R.V. Kesseli, 1991. Identification of marker linked to disease resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci 88: 9828–9832.Google Scholar
  31. Miklas, P.N., S. Lambert, G. Mink & M. Silbernagel, 1998. Many beans with bc-3 resistance to BCMNV are susceptible to BCMV. Annu Rep Bean Improv Coop. 41: 33–34.Google Scholar
  32. Miklas, P.N., R.C. Larsen, R. Riley & J.D. Kelly, 2000. Potential marker assisted selection for bc-12 resistance to bean common mosaic potyvirus in common bean. Euphytica 116: 211–219.Google Scholar
  33. Miklas, P.N., A.N. Hang, J.D. Kelly, C.A. Strausbaugh & R.L. Forster, 2002. Registration of three kidney bean germplasm lines resistant to bean common mosaic virus and necrosis potyviruses: USLK-2 light red kidney, USDK-4 dark red kidney, and USWK-6 white kidney. Crop Sci 42: 674–675.Google Scholar
  34. Miklas, P.N. & J.D. Kelly, 2002. Registration of two cranberry bean germplasm lines resistant to bean common mosaic and necrosis potyviruses: USCR-7 and USCR-9. Crop Sci 42: 673–674.Google Scholar
  35. Njau, P.J.R. & H.F.J. Lyimo, 2000. Incidence of bean common mosaic virus and bean common mosaic necrosis virus in bean (Phaseolus vulgaris L.) in the wild legumes seedlots in Tanzanian Seed. Sci Technol 28: 85–92.Google Scholar
  36. Provvidenti, R., M.J. Silbernagel & W.Y. Wang, 1984. Local epidemic of NL-8 strain of bean common mosaic virus in bean fields of western New York. Plant Dis 68: 1092–1094.Google Scholar
  37. Revers, F., O. Le Gall, T. Candresse & A.J. Maule, 1999. New advances in understanding the molecular biology of plant/potyvirus interactions. Mol Plant-Microbe Interact 12: 367–376.Google Scholar
  38. Sáiz, M., C. De Blas, G. Carazo, J. Fresno, J. Romero & S. Castro, 1995. Incidence and characterization of bean common mosaic virus isolates in Spanish bean fields. Plant Dis 79: 79–81.Google Scholar
  39. Sengooba, T.N., N.J. Spence, D.G.A. Walkey, D.J. Allen & A. Femi Lana, 1997. The occurrence of bean common mosaic necrosis virus in wild and forage legumes in Uganda. Plant Pathol 46: 95–103.Google Scholar
  40. Silbernagel, M.J., L.J. Mills & W.Y. Wang, 1986. Tanzanian strain of bean common mosaic virus. Plant Dis 70: 839–841.Google Scholar
  41. Silbernagel, M.J., G.I. Mink, R.-L. Zhao & G.-Y. Zheng, 2001. Phenotypic recombination between bean common mosaic and bean common mosaic necrosis potyviruses in vivo. Arch Virol 146: 1007–1020.Google Scholar
  42. Spence, N.J. & D.G.A. Walkey, 1995. Variation for pathogenicity among isolates of bean common mosaic virus in Africa and reinterpretation of the genetic relationship between cultivars of Phaseolus vulgaris and pathotypes of BCMV. Plant Pathol 44: 527–546.Google Scholar
  43. Strausbaugh, C.A., J.R. Myers, R.L. Forster & P.E. McClean, 1999. Bc-1 and Bc-u two loci controlling bean common mosaic virus resistance in common bean are linked. J Am Soc Hort Sci 124: 644–648.Google Scholar
  44. Tu, J.C., 1986. Isolation and characterization of a new necrotic strain (NL-8) of bean common mosaic virus in Southwestern Ontario. Can Plant Dis Surv 66: 13–14.Google Scholar
  45. Urrea, C.A., P.N. Miklas, J.S. Beaver & R.H. Riley, 1996. A codominant randomly amplified polymorphic DNA (RAPD) marker useful for indirect selection of bean golden mosaic virus resistance in common bean. J Am Soc Hort Sci 121: 1035–1039.Google Scholar
  46. Vallejo, V. & J.D. Kelly, 2002. The AFLP analysis to tag the Co-12 gene conditioning resistance to bean anthracnose. Plant and animal genome X conference 2002. http://www.int-pag.org/pag/10/abstracts/PAGX P233.html

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Gerardine Mukeshimana
    • 1
  • Astrid Pañeda
    • 2
  • Cristina Rodríguez-Suárez
    • 2
  • Juan José Ferreira
    • 3
  • Ramón Giraldez
    • 2
  • James D. Kelly
    • 4
  1. 1.National University of Rwanda, NURButareRwanda
  2. 2.Department of Biología FuncionalUniversity of OviedoOviedoSpain
  3. 3.SERIDAVillaviciosa (Asturias)Spain
  4. 4.Crop and Soil SciencesMichigan State UniversityEast LansingUSA

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