Construction of a genetic linkage map and QTL analysis of fiber-related traits in upland cotton (Gossypium hirsutum L.)
- 653 Downloads
A genetic linkage map with 70 loci (55 SSR, 12 AFLP and 3 morphological loci) was constructed using 117 F2 plants obtained from a cross between two upland cotton cultivars Yumian 1 and T586, which have relatively high levels of DNA marker polymorphism and differ remarkably in fiber-related traits. The linkage map comprised of 20 linkage groups, covering 525 cM with an average distance of 7.5 cM between two markers, or approximately 11.8% of the recombination length of the cotton genome. The present genetic linkage map was used to identify and map the quantitative trait loci (QTLs) affecting lint percentage and fiber quality traits in 117 F2:3 family lines. Sixteen QTLs for lint percentage and fiber quality traits were identified in six linkage groups by multiple interval mapping: four QTLs for lint percentage, two QTLs for fiber 2.5% span length, three QTLs for fiber length uniformity, three QTLs for fiber strength, two QTLs for fiber elongation and two QTLs for micronaire reading. The QTL controlling fiber-related traits were mainly additive, and meanwhile including dominant and overdominant. Several QTLs affecting different fiber-related traits were detected within the same chromosome region, suggesting that genes controlling fiber traits may be linked or the result of pleiotropy.
Key Wordscotton (Gossypium hirsutum L.) fiber-related traits genetic linkage map QTLs (quantitative trait loci)
Unable to display preview. Download preview PDF.
- Briard, M., V.L. Clerc, D. Grzebelus, D. Senalik & P.W. Simon, 2000. Modified protocols for rapid carrot genome DNA extraction and AFLP analysis using silver stain or radioisotopes. Plant Mol Biol Reporter 18: 235–241.Google Scholar
- Endrizzi, J.E., E.L. Turcotte & R.J. Kohel, 1984. Qualitative genetics, cytology and cytogenetics. In: R.J. Kohel & C.F. Lewis (eds.), Cotton. pp. 81–109, Am Society of Agronomy.Google Scholar
- Fryxell, P.A., 1992. A revised taxonomic interpretation of Gossypium. Rheedea 2: 108–165.Google Scholar
- Iqbal, M.J., O.U.K. Reddy, K.M. El-Zik & A.E. Pepper, 2001. A genetic bottleneck in the ‘evolution under domestication’ of upland cotton Gossypium hirsutum L. examined using DNA fingerprinting. Theor Appl Genet 103: 547–554.Google Scholar
- Kohel, R.J., 1999. Cotton germplasm resources and the potential for improved fiber production and quality. In: A.S. Basra (ed.), Cotton Fibers, pp.167–182, The Haworth Press Inc, New York.Google Scholar
- Kosambi, D.D., 1944. The estimation of map distances from recombination values. Ann Eugen 12: 172–175.Google Scholar
- Meredith Jr, W.R., 1984. Qualitative genetics. In: R.J. Kohel & C.F. Lewis (ed.), Cotton. pp.131–150, Am Society of Agronomy.Google Scholar
- Permingeat, H.R., M.V. Romagnoli & R.H. Vallejos, 1998. A simple method for isolating high yield and quality DNA from cotton (Gossypium hirsutum L.) leaves. Plant Mol Biol Reporter 16: 1–6.Google Scholar
- Reddy, O.U.K., A.E. Pepper, I. Abdurakhmonov, S. Saha, J.N. Jenkins, T. Brooks, Y. Bolek & K.M. El-Zik, 2001. New dinucleotide and trinucleotide microsatellite markers resources for cotton genome research. J Cotton Sci 5: 103–113.Google Scholar
- Rong, J.-K., C. Abbey, J.E. Bowers, C.L. Brubaker, C. Chang, W.-C. Peng, T.A. Delmonte, X.-L. Ding, J.J. Garza, B.S. Marler, C.H. Park, G.J. Pierce, K.M. Rainey, V.K. Rastogi, S.R. Schulze, N.L. Trolinder, J.F. Wendel, T.A. Wilkins, T.D. Williams-Coplin, R.A. Wing, R.J. Wright, X.-P. Zhao, L.-H. Zhu & A.H. Paterson, 2004. A 3347-locus genetic recombination map of sequence-tagged sites reveals features of genome organization, transmission and evolution of cotton (Gossypium). Genetics 166: 389–417.CrossRefPubMedGoogle Scholar
- Schwarz, G., 1978. Estimating the dimension of model. Ann Stat 6: 461–464.Google Scholar
- Shappley, Z.W., J.N. Jenkins, J. Zhu & J.C. McCarty, 1998b. Quantitative trait loci associated with agronomic and fiber traits of upland cotton. J Cotton Sci 2: 153–163.Google Scholar
- Ulloa, M. & W.R. Meredith Jr, 2000. Genetic linkage map and QTL analysis of agronomic and fiber quality traits in an intraspecific population. J Cotton Sci 4: 161–170.Google Scholar
- Van Ooijen, J.W. & R.E. Voorrips, 2001. JoinMap 3.0, Software for the calculation of genetic linkage maps. Plant Research International, Wageningen, the Netherlands.Google Scholar
- Voorrips, R.E., 2002. MapChart 2.1: Software for the graphical presentation of linkage maps and QTLs. Plant Research International, Wageningen, The Netherlands. http://www.plant.wageningen-ur.nl.
- Wang, S.-C., C.J. Basten & Z.-B. Zeng, 2004. Windows QTL Cartographer WinQtlcartV2.0. Statistical Genetics, North Carolina State University. http://statgen.ncsu.edu/qtlcart/WQTLCart.htm.
- Wendel, J.F., C.L. Brubaker & E. Percival, 1992. Genetic diversity in Gossypim hirsutum and the origin of Upland cotton. Am J Bot 79: 1291–1310.Google Scholar