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Euphytica

, Volume 144, Issue 1–2, pp 91–99 | Cite as

Construction of a genetic linkage map and QTL analysis of fiber-related traits in upland cotton (Gossypium hirsutum L.)

  • Zheng-Sheng Zhang
  • Yue-Hua Xiao
  • Ming Luo
  • Xian-Bi Li
  • Xiao-Ying Luo
  • Lei Hou
  • De-Mou Li
  • Yan Pei
Article

Abstract

A genetic linkage map with 70 loci (55 SSR, 12 AFLP and 3 morphological loci) was constructed using 117 F2 plants obtained from a cross between two upland cotton cultivars Yumian 1 and T586, which have relatively high levels of DNA marker polymorphism and differ remarkably in fiber-related traits. The linkage map comprised of 20 linkage groups, covering 525 cM with an average distance of 7.5 cM between two markers, or approximately 11.8% of the recombination length of the cotton genome. The present genetic linkage map was used to identify and map the quantitative trait loci (QTLs) affecting lint percentage and fiber quality traits in 117 F2:3 family lines. Sixteen QTLs for lint percentage and fiber quality traits were identified in six linkage groups by multiple interval mapping: four QTLs for lint percentage, two QTLs for fiber 2.5% span length, three QTLs for fiber length uniformity, three QTLs for fiber strength, two QTLs for fiber elongation and two QTLs for micronaire reading. The QTL controlling fiber-related traits were mainly additive, and meanwhile including dominant and overdominant. Several QTLs affecting different fiber-related traits were detected within the same chromosome region, suggesting that genes controlling fiber traits may be linked or the result of pleiotropy.

Key Words

cotton (Gossypium hirsutum L.) fiber-related traits genetic linkage map QTLs (quantitative trait loci) 

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References

  1. Briard, M., V.L. Clerc, D. Grzebelus, D. Senalik & P.W. Simon, 2000. Modified protocols for rapid carrot genome DNA extraction and AFLP analysis using silver stain or radioisotopes. Plant Mol Biol Reporter 18: 235–241.Google Scholar
  2. Endrizzi, J.E., E.L. Turcotte & R.J. Kohel, 1984. Qualitative genetics, cytology and cytogenetics. In: R.J. Kohel & C.F. Lewis (eds.), Cotton. pp. 81–109, Am Society of Agronomy.Google Scholar
  3. Frary, A., T.C. Nesbitt, A. Frary, S. Grandillo, E. van der Knaap, B. Cong, J. Liu, J. Meller, R. Elber, K.B. Alpert & S.D. Tanksley, 2000. fw2.2: A quantitative trait locus key to the evolution of tomato fruit size. Science 289: 85–88.PubMedGoogle Scholar
  4. Fryxell, P.A., 1992. A revised taxonomic interpretation of Gossypium. Rheedea 2: 108–165.Google Scholar
  5. Iqbal, M.J., O.U.K. Reddy, K.M. El-Zik & A.E. Pepper, 2001. A genetic bottleneck in the ‘evolution under domestication’ of upland cotton Gossypium hirsutum L. examined using DNA fingerprinting. Theor Appl Genet 103: 547–554.Google Scholar
  6. Kao, C.-H. & Z.-B. Zeng, 1997. General formulae for obtaining the MLEs and the asymptotic variance-covariance matrix in mapping quantitative trait loci when using the EM algorithm. Biometrics 53: 653–665.PubMedGoogle Scholar
  7. Kloth, R.H., 1995. Quantitative trait loci affecting cotton fiber are linked to the t1 locus in upland cotton. Theor Appl Genet 91: 762–768.CrossRefGoogle Scholar
  8. Kohel, R.J., 1999. Cotton germplasm resources and the potential for improved fiber production and quality. In: A.S. Basra (ed.), Cotton Fibers, pp.167–182, The Haworth Press Inc, New York.Google Scholar
  9. Kosambi, D.D., 1944. The estimation of map distances from recombination values. Ann Eugen 12: 172–175.Google Scholar
  10. Lacape, J.M., T.B. Nguyen, S. Thibivilliers, B. Bojinov, B. Courtois, R.G. Cantrell, B. Burr & B. Hau, 2003. A combined RFLP–SSR–AFLP map of tetraploid cotton based on a Gossypium hirsutum ×Gossypium barbadense backcross population. Genome 46: 612–626.CrossRefPubMedGoogle Scholar
  11. Liu, S., S. Saha, D.M. Stelly, B. Burr & R.G. Cantrell, 2000. Chromosomal assignment of microsatellite loci in cotton. J Hered 91: 326–332.CrossRefPubMedGoogle Scholar
  12. Meredith Jr, W.R., 1984. Qualitative genetics. In: R.J. Kohel & C.F. Lewis (ed.), Cotton. pp.131–150, Am Society of Agronomy.Google Scholar
  13. Nguyen, T.B., M. Giband, P. Brottier, A.M. Risterucci & J.M. Lacape, 2004. Wide coverage of the tetraploid cotton genome using newly developed microsatellite markers. Theor Appl Genet 109: 167–175.CrossRefPubMedGoogle Scholar
  14. Paterson, A.H., Y. Saranga, M. Menz & C.-X. Jiang, 2003. QTL analysis of genotype × environment interactions affecting cotton fiber quality. Theor Appl Genet 106: 384–396PubMedGoogle Scholar
  15. Permingeat, H.R., M.V. Romagnoli & R.H. Vallejos, 1998. A simple method for isolating high yield and quality DNA from cotton (Gossypium hirsutum L.) leaves. Plant Mol Biol Reporter 16: 1–6.Google Scholar
  16. Reddy, O.U.K., A.E. Pepper, I. Abdurakhmonov, S. Saha, J.N. Jenkins, T. Brooks, Y. Bolek & K.M. El-Zik, 2001. New dinucleotide and trinucleotide microsatellite markers resources for cotton genome research. J Cotton Sci 5: 103–113.Google Scholar
  17. Reinisch, A.J., J.M. Dong, C.L. Brubaker, J.F. Wendel & A.H. Paterson, 1994. A detailed RFLP map of cotton, Gossypium hirsutum × Gossypium barbadense: chromosome organization and evolution in disomic polyploid genome. Genetics 138: 829–847.PubMedGoogle Scholar
  18. Rong, J.-K., C. Abbey, J.E. Bowers, C.L. Brubaker, C. Chang, W.-C. Peng, T.A. Delmonte, X.-L. Ding, J.J. Garza, B.S. Marler, C.H. Park, G.J. Pierce, K.M. Rainey, V.K. Rastogi, S.R. Schulze, N.L. Trolinder, J.F. Wendel, T.A. Wilkins, T.D. Williams-Coplin, R.A. Wing, R.J. Wright, X.-P. Zhao, L.-H. Zhu & A.H. Paterson, 2004. A 3347-locus genetic recombination map of sequence-tagged sites reveals features of genome organization, transmission and evolution of cotton (Gossypium). Genetics 166: 389–417.CrossRefPubMedGoogle Scholar
  19. Schwarz, G., 1978. Estimating the dimension of model. Ann Stat 6: 461–464.Google Scholar
  20. Shappley, Z.W., J.N. Jenkins, W.R. Meredith Jr & J.C. McCarty, 1998a. An RFLP linkage map of upland cotton, Gossypium hirsutum L. Theor Appl Genet 97: 756–761.CrossRefGoogle Scholar
  21. Shappley, Z.W., J.N. Jenkins, J. Zhu & J.C. McCarty, 1998b. Quantitative trait loci associated with agronomic and fiber traits of upland cotton. J Cotton Sci 2: 153–163.Google Scholar
  22. Tanksley, S.D. & J. Hewitt, 1988. Use of molecular markers in breeding for soluble solids content in tomato – a re-examination. Theor Appl Genet 75: 811–823.CrossRefGoogle Scholar
  23. Ulloa, M. & W.R. Meredith Jr, 2000. Genetic linkage map and QTL analysis of agronomic and fiber quality traits in an intraspecific population. J Cotton Sci 4: 161–170.Google Scholar
  24. Ulloa, M., W.R. Meredith Jr, Z.W. Shappley & A.L. Kahler, 2002. RFLP genetic linkage maps from four F2.3 populations and a joinmap of Gossypium hirsutum L. Theor Appl Genet 104: 200–208.CrossRefPubMedGoogle Scholar
  25. Van Ooijen, J.W. & R.E. Voorrips, 2001. JoinMap 3.0, Software for the calculation of genetic linkage maps. Plant Research International, Wageningen, the Netherlands.Google Scholar
  26. Voorrips, R.E., 2002. MapChart 2.1: Software for the graphical presentation of linkage maps and QTLs. Plant Research International, Wageningen, The Netherlands. http://www.plant.wageningen-ur.nl.
  27. Vos, P., R. Hogers & M. Bleeker, 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acids Res 23: 4407–4414.PubMedGoogle Scholar
  28. Wang, S.-C., C.J. Basten & Z.-B. Zeng, 2004. Windows QTL Cartographer WinQtlcartV2.0. Statistical Genetics, North Carolina State University. http://statgen.ncsu.edu/qtlcart/WQTLCart.htm.
  29. Wendel, J.F., C.L. Brubaker & E. Percival, 1992. Genetic diversity in Gossypim hirsutum and the origin of Upland cotton. Am J Bot 79: 1291–1310.Google Scholar
  30. Wright, R.J., P.M. Thaxton, K.M. El-Zik & A.H. Paterson, 1998. D-Subgenome bias of Xcm resistance genes in tetraploid Gossypium (cotton) suggests that polyloid formation has created novel avenues for evolution. Genetics 149: 1987–1996.PubMedGoogle Scholar
  31. Xiao, J., S. Grandillo, S.N. Ahn, S.R. McCouch, S.D. Tanskley, J. Li & L. Yuan, 1996. Genes from wild rice improve yield. Nature 384: 223–224.CrossRefGoogle Scholar
  32. Zhang, J., W.-Z. Guo & T.-Z. Zhang, 2002. Molecular linkage map of allotetraploid (Gossypium hirsutum L. × Gossypium barbadense L.) with a haploid population. Theor Appl Genet 105: 1166–1174.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Zheng-Sheng Zhang
    • 1
  • Yue-Hua Xiao
    • 1
  • Ming Luo
    • 1
  • Xian-Bi Li
    • 1
  • Xiao-Ying Luo
    • 1
  • Lei Hou
    • 1
  • De-Mou Li
    • 1
  • Yan Pei
    • 1
  1. 1.Key Laboratory of Biotechnology & Crop Quality Improvement, Ministry of AgricultureSouthwest Agricultural UniversityChongqingP.R. China

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