Euphytica

, Volume 145, Issue 3, pp 269–279 | Cite as

QTL mapping of volatile compounds in ripe apples detected by proton transfer reaction-mass spectrometry

  • Elena Zini
  • Franco Biasioli
  • Flavia Gasperi
  • Daniela Mott
  • Eugenio Aprea
  • Tilmann D. Märk
  • Andrea Patocchi
  • Cesare Gessler
  • Matteo Komjanc
Article

Abstract

The availability of genetic linkage maps enables the detection and analysis of QTLs contributing to quality traits of the genotype. Proton Transfer Reaction Mass Spectrometry (PTR-MS), a relatively novel spectrometric technique, has been applied to measure the headspace composition of the Volatile Organic Compounds (VOCs) emitted by apple fruit genotypes of the progeny ‘Fiesta’ × ‘Discovery’. Fruit samples were characterised by their PTR-MS spectra normalised to total area. QTL analysis for all PTR-MS peaks was carried out and 10 genomic regions associated with the peaks at m/z = 28, 43, 57, 61, 103, 115 and 145 were identified (LOD > 2.5). We show that it is possible to find quantitative trait loci (QTLs) related to PTR-MS characterisation of the headspace composition of single whole apple fruits indicating the presence of a link between molecular characterisation and PTR-MS data. We provide tentative information on the metabolites related to the detected QTLs based on available chemical information. A relation between apple skin colour and peaks related to carbonyl compounds was established.

Keywords

linkage map Malus on-line headspace analysis PTR-MS QTLs Volatile Organic Compounds 

Abbreviation

ANOVA:

analysis of variance

bp:

base pairs

IM:

Interval Mapping

KW:

Kruskal Wallis

LG:

linkage group

LOD:

Logarithm of the odds

m/z:

mass charge ratio

ppbv:

part per billion by volume

PTR-MS:

Proton Transfer Reaction- Mass Spectrometry

QTLs:

Quantitative Trait Loci

RAPD:

Random Amplified Polymorphism DNA

SSR:

Simple Sequence Repeats

VOCs:

Volatile Organic Compounds

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Elena Zini
    • 1
  • Franco Biasioli
    • 1
  • Flavia Gasperi
    • 1
  • Daniela Mott
    • 1
  • Eugenio Aprea
    • 1
    • 2
  • Tilmann D. Märk
    • 2
    • 3
  • Andrea Patocchi
    • 4
  • Cesare Gessler
    • 4
    • 5
  • Matteo Komjanc
    • 1
  1. 1.Istituto Agrario di S. Michele all'AdigeS. Michele all'AdigeItaly
  2. 2.Institut für IonenphysikUniversität InnsbruckInnsbruckAustria
  3. 3.Department of PlasmaphysicsUniversity of BratislavaBratislavaSlovak Republic
  4. 4.Institute of Plant SciencesSwiss Federal Institute of TechnologyZürichSwitzerland
  5. 5.SafeCrop centerIstituto Agrario di S. Michele all'AdigeS. Michele all'AdigeItaly

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