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Euphytica

, Volume 142, Issue 1–2, pp 131–136 | Cite as

Simple sequence repeat markers for botanical varieties of cultivated peanut (Arachis hypogaea L.)

  • Guohao He
  • Ronghua Meng
  • Hui Gao
  • Baozhu Guo
  • Guoqing Gao
  • Melanie Newman
  • Roy N Pittman
  • C. S. Prakash
Article

Abstract

Cultivated peanut (Arachis hypogaea L.) consists of six botanical varieties. Identification of DNA markers associated with botanical varieties would be useful in plant genotyping, germplasm management, and evolutionary studies. We have developed 130 simple sequence repeat (SSR) markers in peanut, 38 of which were used in this study because of their ability in detecting genetic polymorphism among 24 peanut accessions. Eight SSR markers were found useful to classify botanical varieties. Among them, six SSR markers were specific to botanical varieties fastigiata and vulgaris, one to botanical varieties hypogaea and hirsuta, and one to botanical varieties peruviana, and aequatoriana. Also, three of them derived from peanut expressed sequence tags (ESTs) were associated with putative genes. As botanical varieties have different morphological traits and belong to different subspecies in A. hypogaea, these markers might be associated with genes involved in the expression of morphological traits. The results also suggested that SSRs (also called microsatellites) might play a role in shaping evolution of cultivated peanut. Multiplex PCR of botanical variety-specific markers could be applied to facilitate efficient genotyping of the peanut lines.

Keywords

botanical variety identification peanut simple sequence repeat (SSR) 

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Guohao He
    • 1
  • Ronghua Meng
    • 1
  • Hui Gao
    • 1
  • Baozhu Guo
    • 2
  • Guoqing Gao
    • 3
  • Melanie Newman
    • 4
  • Roy N Pittman
    • 4
  • C. S. Prakash
    • 1
  1. 1.Department of Agricultural SciencesTuskegee UniversityTuskegee
  2. 2.USDA-ARSCrop Protection and Management Research UnitTifton
  3. 3.Guangxi Academy of Agricultural SciencesNanningChina
  4. 4.USDA-ARSPlant Genetic Resources Conservation UnitGriffin

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