Euphytica

, Volume 139, Issue 1, pp 59–64 | Cite as

Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai

  • Xu Zhang
  • Miaoping Zhou
  • Lijuan Ren
  • Guihua Bai
  • Hongxiang Ma
  • Olga E Scholten
  • Peiguo Guo
  • Weizhong Lu
Article

Abstract

Fusarium head blight (FHB) is a destructive disease of wheat worldwide. FHB resistance genes from Sumai 3 and its derivatives such as Ning 7840 have been well characterized through molecular mapping. In this study, resistance genes in Wangshuibai, a Chinese landrace with high and stable FHB resistance, were analyzed through molecular mapping. A population of 104 F2-derived F7 recombinant inbred lines (RILs) was developed from the cross between resistant landrace Wangshuibai and susceptible variety Alondra‘s’. A total of 32 informative amplified fragment length polymorphism (AFLP) primer pairs (EcoRI/MseI) amplified 410 AFLP markers segregating among the RILs. Among them, 250 markers were mapped in 23 linkage groups covering a genetic distance of 2,430 cM. In addition, 90 simple sequence repeat (SSR) markers were integrated into the AFLP map. Fifteen markers associated with three quantitative trait loci (QTL) for FHB resistance (P < 0.01) were located on two chromosomes. One QTL was mapped on 1B and two others were mapped on 3B. One QTL on 3BS showed a major effect and explained up to 23.8% of the phenotypic variation for type II FHB resistance.

amplified fragment length polymorphisms AFLP Fusarium head blight simple sequence repeat Triticum aestivum L. 

Abbreviations

cM

centimorgan

LOD

log likelihood ratio

RILs

recombinant inbred lines

AFLP

amplified fragment length polymorphisms

FHB

Fusarium head blight

SSR

simple sequence repeat

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Xu Zhang
    • 1
  • Miaoping Zhou
    • 1
  • Lijuan Ren
    • 1
  • Guihua Bai
    • 2
  • Hongxiang Ma
    • 1
  • Olga E Scholten
    • 3
  • Peiguo Guo
    • 4
  • Weizhong Lu
    • 1
  1. 1.Institute of Agrobiological Genetics and PhysiologyJiangsu Academy of Agricultural SciencesNanjingP.R. China
  2. 2.USDA/ARS/PSERU, 4008 Throckmorton Hall, Department of AgronomyKansas State UniversityU.S.A.
  3. 3.BU Genetics and BreedingPlant Research InternationalWageningenThe Netherlands
  4. 4.Department of Plant and Soil SciencesOklahoma State UniversityU.S.A.

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