Optimization of QuPPe approach and validation of analytical method for estimation of validamycin from grain, paddy husk, and soil by HPLC-ESI-MS/MS-based method

  • Bharat Bhusan SubudhiEmail author
  • Nihar Ranjan Muduli
  • Prabhat Kumar Behera
  • Alok Kumar Moharana
  • Rajanikanta SahuEmail author


Validamycin is the most active component of the antibiotic and antifungal validamycin complex. Since it is widely used to protect rice crops, its persistence in soil and rice matrices may affect the quality and the health of the consumer. Methods for its estimation from soil matrices are not available. Besides, its analysis from complex matrices including grain and paddy husk is challenging. So very few reliable analytical methods are available for its residue analysis. Thus, we aimed to develop a new QuPPe technique and new HPLC-ESI-MS/MS analytical method for the estimation of validamycin in grain, paddy husk, and soil. The QuPPe method was validated with a linearity range of 0.00101 to 0.10134 μg/mL. The LOQ of validamycin in grain, paddy husk, and soil was 1.01013 μg/kg and the retention time was 5.62 min. These results will be helpful in further analysis of food safety and environmental pollution.


Validamycin HPLC Rice QuPPe 



The authors are thankful to Krish Biotech Research Pvt. Ltd. for providing the support to complete the study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Not applicable

Supplementary material

10661_2019_8006_MOESM1_ESM.docx (1.1 mb)
ESM 1 (DOCX 1133 kb)


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Drug Development and Analysis Lab., School of Pharmaceutical SciencesSiksha O Anusandhan Deemed to be UniversityBhubaneswarIndia
  2. 2.Krish Biotech Research Pvt. Ltd.KalyaniIndia

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