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A new multistep purification method for simultaneously determining organic ultraviolet absorbents in fish tissue

  • Fuyong Zhong
  • Xianhui Zhang
  • Guanghui Li
  • Zhenwu TangEmail author
  • Xue Han
  • Jiali ChengEmail author
Article
  • 44 Downloads

Abstract

More information is required to improve our understanding of the presence of organic ultraviolet absorbents (UVAs) in organisms and the risks posed to biota. Effective removal of lipids in the determination of UVAs in biological samples is a sample-processing bottleneck. In this study, we optimized a multistep purification method for fish tissue samples extracted using an ultrasonic-assisted extraction method. The purification method involved performing Florisil column chromatography, redissolving the extract in ethanol, and then performing dispersive solid-phase extraction using primary and secondary amine, Florisil, and C18 silica sorbents. The purified samples were analyzed by gas chromatography–mass spectrometry. The method was effective and reliable, and was used to detect trace concentrations (ng/g) of 12 UVAs in fish tissue samples. The mean recovery range of the UVAs in fish tissue extracts was 65.4–118%, and the method detection limit range was 0.20–2.50 ng/g dw. The validated method was used to analyze 12 UVAs in fish samples from a local supermarket.

Keywords

Organic ultraviolet absorbent Dispersive solid-phase extraction Multistep purification Gas chromatography–mass spectrometry Fish 

Notes

Funding information

This research was supported by the National Natural Science Foundation of China (Grant Nos. 41571445; 41877467) and the Fundamental Research Funds for the Central Universities (Grant No. 15MS60).

Supplementary material

10661_2018_7139_MOESM1_ESM.docx (204 kb)
ESM 1 (DOCX 203 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.College of Life and Environmental SciencesMinzu University of ChinaBeijingChina
  2. 2.MOE Key Laboratory of Resources and Environmental System Optimization, College of Environmental Science and EngineeringNorth China Electric Power UniversityBeijingChina
  3. 3.Agricultural Product Quality Safety Inspection and Testing Center of HuainanHuainanChina
  4. 4.China Merchants Ecological Environmental Protection Technology Co., Ltd.ChongqingChina
  5. 5.Key Laboratory of Trace Element Nutrition of National Health CommissionNational Institute for Nutrition and Health, Chinese Center for Disease Control and PreventionBeijingChina

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