Identification and detection sensitivity of Microcystis aeruginosa from mixed and field samples using MALDI-TOF MS
To verify the applicability of identifying Microcystis aeruginosa by matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry (MALDI-TOF MS), mixed and field samples were employed to study the sensitivity and the analysis power, respectively. Series diluted samples and artificially mixed samples by the M. aeruginosa NIES-843 strain were designed to verify the sensitivity. The lowest detection limit was 1.955 × 106 cells in pure samples, while for mixed samples, the lowest detection limit and ratio of NIES-843 strain were 2.88 × 106 cells and 33.7%, respectively. The results provided a reference for the reasonable volume of the water sample in which the M. aeruginosa could be detected. Ribosomal protein biomarkers for identifying M. aeruginosa which were successfully detected from the field samples in Taihu Lake, indicated that the identification of M. aeruginosa by MALDI-TOF MS could be applied in field samples. Furthermore, different genetic types of M. aeruginosa strains were also detected at different locations in Taihu Lake, which revealed the diversity of M. aeruginosa and the detection power of MALDI-TOF MS at the strain level for the field samples. The sensitivity and detection power in the analysis of M. aeruginosa by the MALDI-TOF MS demonstrated the applicability of this method in routine environmental monitoring.
KeywordsMicrocystis aeruginosa MALDI-TOF MS Cyanobacterial bloom Ribosomal protein
The present study was supported by the National Key Research and Development Program - China (2016YFB0601003). We would like to express our gratitude toward Dr. Kosei Yumoto at MCC-NIES, Japan, for providing cultures of all cyanobacteria strains. We thank Dr. Noriko Takamura for her cooperation in the present research.
Li-Wei Sun and Wen-Jing Jiang conceived and designed the experiments; Li-Wei Sun, Wen-Jing Jiang, and Yang Du performed the experiment; Jun-Yi Zhang and Wen-Qian Wang partly performed the experiments; Wen-Jing Jiang, Masanobu Kawachi, and Li-Wei Sun analyzed the data; Ran Yu conceived part of the experiment and prepared part of the manuscript; Hiroaki Sato contributed to the analysis of data and improved the manuscript; Li-Wei Sun wrote the paper.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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