Simultaneous measurements of dissolved CH4 and H2 in wetland soils

  • David S. Pal
  • Rajan Tripathee
  • Matthew C. Reid
  • Karina V. R. Schäfer
  • Peter R. Jaffé


Biogeochemical processes in wetland soils are complex and are driven by a microbiological community that competes for resources and affects the soil chemistry. Depending on the availability of various electron acceptors, the high carbon input to wetland soils can make them important sources of methane production and emissions. There are two significant pathways for methanogenesis: acetoclastic and hydrogenotrophic methanogenesis. The hydrogenotrophic pathway is dependent on the availability of dissolved hydrogen gas (H2), and there is significant competition for available H2. This study presents simultaneous measurements of dissolved methane and H2 over a 2-year period at three tidal marshes in the New Jersey Meadowlands. Methane reservoirs show a significant correlation with dissolved organic carbon, temperature, and methane emissions, whereas the H2 concentrations measured with dialysis samplers do not show significant relationships with these field variables. Data presented in this study show that increased dissolved H2 reservoirs in wetland soils correlate with decreased methane reservoirs, which is consistent with studies that have shown that elevated levels of H2 inhibit methane production by inhibiting propionate fermentation, resulting in less acetate production and hence decreasing the contribution of acetoclastic methanogenesis to the overall production of methane.


Methane Hydrogen Wetlands Sediments 


Funding information

This work was supported by the CBET-1133074, CBET 1033639, CBET 1133275, CBET 1311713, CBET 1033451, CBET 1311547, and CBET-1133281, NSF Collaborative Research: RAPID Award no. 1311796.


  1. Achtnich, C., Friedhelm, B., & Conrad, R. (1995). Competition for electron donors among nitrate reducers, ferric iron reducers, sulfate reducers, and methanogens in anoxic paddy soil. Biol Fertil Soils Biology and Fertility of Soils, 19(1), 65–72.CrossRefGoogle Scholar
  2. Altor, A., & Mitsch, W. J. (2006). Methane flux from created riparian marshes: relationship to intermittent versus continuous inundation and emergent macrophytes. Ecological Engineering, 28(3), 224–234.CrossRefGoogle Scholar
  3. Bridgham, S., Cadillo-Quiroz, H., Keller, J. K., & Zhuang, Q. (2013). Methane emissions from wetlands: biogeochemical, microbial, and modeling perspectives from local to global scales. Global Change Biology, 19(5), 1325–1346.CrossRefGoogle Scholar
  4. Brook E, Archer D, Dlugokencky E, Frolking S, Lawrence D (2008). Potential for abrupt changes in atmospheric methane. Abrupt climate change: A report by the U.S. Climate Change Science Program and the Subcommittee on Global Change Research. U.S. Geological Survey, Reston, VA, 360–452.Google Scholar
  5. Brown, D. G., Komlos, J., & Jaffé, P. R. (2005). Simultaneous utilization of acetate and hydrogen by Geobacter sulfurreducens and implications for use of hydrogen as an indicator of redox conditions. Environmental Science & Technology, 39(9), 3069–3076.CrossRefGoogle Scholar
  6. Chin, K. J., & Conrad, R. (1995). Intermediary metabolism in methanogenic paddy soil and the influence of temperature. FEMS Microbio. Eco., 18, 85–102.CrossRefGoogle Scholar
  7. Conrad, R., Bak, F., Seitz, H. J., Thebrath, B., Mayer, H. P., & Schutz, H. (1989). Hydrogen turnover by psychotropic homoacetogenic and mesophilic methanogenic bacteria in anoxic paddy soil and lake sediment. FEMS Microbio. Ecology, 62, 285–294.CrossRefGoogle Scholar
  8. Conrad, R., Klose, M., & Claus, P. (2002). Pathway of CH4 formation in anoxic rice field soil and rice roots determined by 13C-stable isotope fractionation. Chemosphere, 47(8), 797–806.CrossRefGoogle Scholar
  9. ElBishlawi, H., Shin, J. Y., & Jaffe, P. R. (2013). Trace metal dynamics in the sediments of a constructed and natural urban tidal marsh: the role of iron, sulfide, and organic complexation. Ecological Engineering, 58, 133–141.CrossRefGoogle Scholar
  10. Glissmann, K., & Conrad, R. (2000). Fermentation pattern of methanogenic degradation of rice straw in anoxic paddy soil. FEMS Microbiology Ecology, 31(2), 117–126.CrossRefGoogle Scholar
  11. Glissman, K., Chin, K. J., Casper, P., & Conrad, R. (2004). Methanogenic pathway and archael community structure in the sediment of eutrophic Lake Dagow: effect of temperature. Microbial Ecology, 48, 389–399.CrossRefGoogle Scholar
  12. Hesslein, R. (1976). An in situ sampler for close interval pore water studies. Limnology and Oceanography, 21(6), 912–914.CrossRefGoogle Scholar
  13. Karadagli, F., & Rittmann, B. E. (2007). Thermodynamic and kinetic analysis of the H2 threshold for Methanobacterium bryantii M.o.H. Biodegradation, 18, 439–452.CrossRefGoogle Scholar
  14. Komlos, J., & Jaffé, P. R. (2004). Effect of iron bioavailability on dissolved hydrogen concentrations during microbial iron reduction. Biodegradation, 15(5), 315–325.CrossRefGoogle Scholar
  15. Laanbroek, H. J. (2009). Methane emission from natural wetlands: interplay between emergent macrophytes and soil microbial processes. A mini-review. Annals of Botany, 105(1), 141–153.CrossRefGoogle Scholar
  16. Li, T., Huang, Y., Zhang, W., & Song, C. (2010). CH4MODwetland: a biogeophysical model for simulating methane emissions from natural wetlands. Ecological Modeling, 221(4), 666–680.CrossRefGoogle Scholar
  17. Liu, F., & Conrad, R. (2011). Chemolithotrophic acetogenic H2/CO2 utilization in Italian rice field soil. The ISME Journal, 5, 1526–1539.CrossRefGoogle Scholar
  18. Macdonald, L., Paull, J. S., & Jaffé, P. R. (2012). Enhanced semipermanent dialysis samplers for long-term environmental monitoring in saturated sediments. Environmental Monitoring and Assessment, 185(5), 3613–3624.CrossRefGoogle Scholar
  19. Pal, D., & Jaffé, P. R. (2016). Modeling the inhibition of dissolved H2 on propionate fermentation and methanogenesis in wetland sediments. Ecological Modelling, 322, 115–123.CrossRefGoogle Scholar
  20. Pal, D. S., Reid, M. C., & Jaffé, P. R. (2014). Impact of hurricane sandy on CH 4 released from vegetated and unvegetated wetland microsites. Environmental Science & Technology Letters, 1(9), 372–375.CrossRefGoogle Scholar
  21. Reid, M. C., & Jaffé, P. R. (2012). Gas-phase and transpiration-driven mechanisms for volatilization through wetland macrophytes. Environmental Science & Technology, 46(10), 5344–5352.CrossRefGoogle Scholar
  22. Reid, M. C., & Jaffé, P. R. (2013). A push–pull test to measure root uptake of volatile chemicals from wetland soils. Environmental Science & Technology, 47(7), 3190–3198.Google Scholar
  23. Reid, M. C., Tripathee, R., Schäfer, K. V. R., & Jaffé, P. R. (2013). Tidal marsh methane dynamics: difference in seasonal lags in emissions driven by storage in vegetated versus unvegetated sediments. Journal of Geophysical Research – Biogeosciences, 118, 1802–1813.CrossRefGoogle Scholar
  24. Schutz, H., Conrad, R., Goodwin, S., & Sieler, W. (1988). Emission of hydrogen from deep and shallow freshwater environments. Biogeochemisty, 5, 295–311.CrossRefGoogle Scholar
  25. Whiting, G. J., & Chanton, J. P. (1993). Primary production control of methane emission from wetlands. Nature, 364, 794–795.CrossRefGoogle Scholar
  26. Whiting, G. J., & Chanton, J. P. (2001). Greenhouse carbon balance of wetlands: methane emission versus carbon sequestration. Tellus, B53(5), 521–528.Google Scholar
  27. Xu, S., Jaffé, P. R., & Mauzerall, D. L. (2007). A process-based model for methane emission from flooded rice paddy systems. Ecological Modelling, 205, 475–491.CrossRefGoogle Scholar
  28. Yamamoto, A., Hirota, M., Suzuki, S., Oe, Y., Zhang, P., & Mariko, S. (2009). Effects of tidal fluctuations on CO2 and CH4 fluxes in the littoral zone of a brackish-water Lake. Limnology, 10(3), 229–237.CrossRefGoogle Scholar
  29. Yavitt, J. B. (2010). Cryptic wetlands. Nature Geoscience, 3, 749–750.CrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • David S. Pal
    • 1
  • Rajan Tripathee
    • 2
  • Matthew C. Reid
    • 3
  • Karina V. R. Schäfer
    • 2
  • Peter R. Jaffé
    • 1
  1. 1.Department of Civil and Environmental EngineeringPrinceton UniversityPrincetonUSA
  2. 2.Department of Biological SciencesRutgers UniversityNewarkUSA
  3. 3.School of Civil and Environmental EngineeringCornell UniversityIthacaUSA

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