Application of ligninolytic potentials of a white-rot fungus Ganoderma lucidum for degradation of lindane

  • Harsimran Kaur
  • Shammi Kapoor
  • Gaganjyot Kaur


Lindane, a broad-spectrum organochlorine pesticide, has caused a widespread environmental contamination along with other pesticides due to wrong agricultural practices. The high efficiency, sustainability and eco-friendly nature of the bioremediation process provide an edge over traditional physico-chemical remediation for managing pesticide pollution. In the present study, lindane degradation was studied by using a white-rot fungus, Ganoderma lucidum GL-2 strain, grown on rice bran substrate for ligninolytic enzyme induction at 30 °C and pH 5.6 after incorporation of 4 and 40 ppm lindane in liquid as well as solid-state fermentation. The estimation of lindane residue was carried out by gas chromatography coupled to mass spectrometry (GC-MS) in the selected ion monitoring mode. In liquid-state fermentation, 100.13 U/ml laccase, 50.96 U/ml manganese peroxidase and 17.43 U/ml lignin peroxidase enzymes were obtained with a maximum of 75.50 % lindane degradation on the 28th day of incubation period, whereas under the solid-state fermentation system, 156.82 U/g laccase, 80.11 U/g manganese peroxidase and 18.61 U/g lignin peroxidase enzyme activities with 37.50 % lindane degradation were obtained. The lindane incorporation was inhibitory to the production of ligninolytic enzymes and its own degradation but was stimulatory for extracellular protein production. The dialysed crude enzyme extracts of ligninolytic enzymes were though efficient in lindane degradation during in vitro studies, but their efficiencies tend to decrease with an increase in the incubation period. Hence, lindane-degrading capabilities of G. lucidum GL-2 strain make it a potential candidate for managing lindane bioremediation at contaminated sites.


Biodegradation Fermentation Ganoderma lucidum Ligninolytic enzymes Lindane 



This research was supported by Punjab Agricultural University (Department of Microbiology), Ludhiana. The authors are indebted to their parent institution for providing a favourable and conducive environment for professional and academic growth.


  1. Bhatia, G., Srivastava, A., & Srivstava, P. C. (2011). Degradation of lindane (γ-HCH) in a Mollisol as affected by different soil amendments. Journal of Environmental Protection, 2, 1207–1210.CrossRefGoogle Scholar
  2. D’Souza, T. M., Merritt, C. S., & Reddy, C. A. (1999). Lignin-modifying enzymes of the white-rot basidiomycete Ganoderma lucidum. Applied and Environmental Microbiology, 65(12), 5307–5313.Google Scholar
  3. Diaz, E. (2010). Microbial biodegradation: genomics and molecular biology (1st ed.). Norfolk: Caister Academic Press.Google Scholar
  4. Dritsa, V., & Rigas, F. (2013). The ligninolytic and biodegradation potential on lindane of Pleurotus ostreatus spp. Journal of Mining World Express, 2(1), 23–30.Google Scholar
  5. Dritsa, V., Rigas, F., Doulia, D., Avramides, E. J., & Hatzianestis, I. (2009). Optimization of culture conditions for the biodegradation of lindane by the polypore fungus Ganoderma australe. Water, Air and Soil Pollution, 204(1), 19–27.CrossRefGoogle Scholar
  6. Elcey, C. D., & Kunhi, A. A. M. (2010). Substantially enhanced degradation of hexachlorocyclohexane isomers by a microbial consortium on acclimation. Journal of Agricultural and Food Chemistry, 58, 1046–1054.CrossRefGoogle Scholar
  7. Gavrilescu, M. (2005). Fate of pesticides in the environment and its biodegradation. Engineering in. Life Sciences, 5(6), 497–527.CrossRefGoogle Scholar
  8. Hattaka, A. (1994). Lignin-modifying enzymes production and role in lignin degradation. FEMS Microbiological Reviews, 13, 125–135.CrossRefGoogle Scholar
  9. Hussaini, S. Z., Shaker, M., & Iqbal, M. A. (2013). Isolation of fungal isolates for degradation of selected pesticides. Bulletin of Environment, Pharmacology and Life Sciences, 2(4), 50–53.Google Scholar
  10. Jo, W. S., Park, H. N., Cho, D. H., Yoo, Y. B., & Park, S. C. (2011). Detection of extracellular enzyme activities in Ganoderma neo-japonicum. Mycobiology, 39, 118–120.CrossRefGoogle Scholar
  11. Kaur, K. (2013). Development of lignin and silica solubilizing microbial consortium for paddy straw pretreatment to enhance biogas production. Punjab: Ph.D. Dissertation, Punjab Agricultural University, Ludhiana.Google Scholar
  12. Kaur, H., Sharma, S., Khanna, P. K., & Kapoor, S. (2015). Evaluation of Ganoderma lucidum strains for the production of bioactive components and their potential use as antimicrobial agents. Journal of Applied and Natural Science, 7(1), 298–303.Google Scholar
  13. Kersten, P., & Cullen, D. (2007). Extracellular oxidative systems of the lignin-degrading basidiomycete Phanerochaete chrysosporium. Fungal Genetics and Biology, 44, 77–87.CrossRefGoogle Scholar
  14. Lang, E., Nerud, F., & Zadrazil, F. (1998). Production of ligninolytic enzymes by Pleurotus sp and Dichomitus squalens in soil and lignocellulose substrate as influence by soil microorganisms. FEMS Microbiology Letters, 167(2), 239–244.CrossRefGoogle Scholar
  15. Li, Y. F. (1999). Global technical hexachlorocyclohexane usage and its contamination consequences in the environment: from 1948 to 1997. Science of the Total Environment, 232, 147–158.CrossRefGoogle Scholar
  16. Lowry, O. H., Rosenbrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.Google Scholar
  17. MacRae, I. C., Yamaya, Y., & Yashida, T. (1984). Persistence of hexachlorocyclohexane isomers in soil suspensions. Soil Biology and Biochemistry, 16, 285–286.CrossRefGoogle Scholar
  18. Maeda, Y., Kajiwara, S., & Ohtaguchi, K. (2001). Manganese peroxidase gene of the perennial mushroom Elfvingia applanata: cloning and evaluation of its relationship with lignin degradation. Biotechnology Letters, 23(2), 103–109.CrossRefGoogle Scholar
  19. Marco-Urrea, E., & Reddy, C. A. (2012). Degradation of chloro-organic pollutants by white rot fungi. In S. N. Singh (Ed.), Microbial degradation of xenobiotics, environmental science and engineering (pp. 31–66). Berlin Heidelberg: Springer.CrossRefGoogle Scholar
  20. Mohammed, A. I., & Brtakke, K. V. (2014). Isolation of pesticide degrading microorganisms from soil. Advances in Bioresearch, 5(4), 164–168.Google Scholar
  21. Mougin, C., Pricaud, C., Dubroca, J., & Asther, M. (1997). Enhanced mineralization of lindane in soils supplemented with the white rot basidiomycetes Phanerochaete chrysosporium. Soil Biology and Biochemistry, 29, 1321–1324.CrossRefGoogle Scholar
  22. Natal-da-Luz, T., Tidona, S., Jesus, B., Morais, P. V., & Souse, J. P. (2009). The use of sewage sludge as soil amendment: the need for an ecotoxicological evaluation. Journal of Soils Sediments., 9(3), 246–260.CrossRefGoogle Scholar
  23. Nuhoglu, A., & Yalcin, B. (2008). Modeling of phenol removal in a batch reactor. Process Biochemistry, 40, 233–239.Google Scholar
  24. Paszezynski, A. J., Ronald, L. C., & Van, B. H. (1988). Manganese peroxidase of Phanerochaete chrysosporium. Methods of Enzymology, 161, 264–270.CrossRefGoogle Scholar
  25. Phillips, T. M., Seech, A. G., Lee, H., & Trevors, J. T. (2005). Biodegradation of hexachlorocyclohexane (HCH) by microorganisms. Biodegradation, 16, 363–392.CrossRefGoogle Scholar
  26. Pointing, S. B. (2001). Feasibility of bioremediation by white rot fungi. Applied Microbiology and Biotechnology, 57(1–2), 20–33.Google Scholar
  27. Quintero, J. C., Moreira, M. T., Feijoo, G., & Lema, J. M. (2008). Screening of white rot fungi species for their capacity to degrade lindane and other isomers of hexachlorocyclohexane (HCH). Ciencia e Investigación Agraria, 32(2), 159–167.Google Scholar
  28. Reddy, C. A., & Mathew, Z. (2001). Bioremediation potential of white rot fungi. In G. M. Gadd (Ed.), Fungi in bioremediation (pp. 52–78). London: Cambridge University Press.CrossRefGoogle Scholar
  29. Rigas, F., Dritsa, V., Marchant, R., Papadopoulou, K., Avramides, E. J., & Hatziannestis, I. (2005). Biodegradation of lindane by Pleurotus ostreatus via central composite design. Environment International, 31(2), 191–196.CrossRefGoogle Scholar
  30. Rigas, F., Papadopoulou, K., Dritsa, V., & Doulia, D. (2007). Bioremediation of a soil contaminated by lindane utilizing the fungus Ganoderma australe via response surface methodology. Journal of Hazardous Materials, 104, 325–332.CrossRefGoogle Scholar
  31. Sahoo, S. K., & Singh, B. (2015). Degradation and downward movement of lindane in soil under cultivated field conditions. Advances in Crop Science and Technology, 4(1), 1000198.CrossRefGoogle Scholar
  32. Sahoo, S. K., Kousik, M., Kumar, R., Singh, B., & Battu, R. S. (2011). Presistence of chlorpyrifos and lindane under moisture conditions. Annals of Plant Protection Sciences, 19(1), 199–202.Google Scholar
  33. Sahu, S. K., Patnaik, K. K., Bhuyan, S., & Sethunathan, N. (1993). Degradation of soil applied isomers of hexachlorocyclohexane by a Pseudomonas sp. Soil Biology and Biochemistry, 25, 387–391.CrossRefGoogle Scholar
  34. Salam, J. A., & Das, N. (2012). Remediation of lindane from environment—a review. International Journal of Advances in Biological Research, 2(1), 9–15.Google Scholar
  35. Schoen, S., & Winterlin, W. (1987). The effects of various soil factors and amendments on the degradation of pesticide mixture. Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Waste, 22(3), 347–377.CrossRefGoogle Scholar
  36. Singh, R. P., Garcha, H. S., & Khanna, P. K. (1988). Laccase production by Pleurotus spp. Indian Journal of Microbiology, 24, 38–41.Google Scholar
  37. Tien, M., & Kirk, T. K. (1988). Lignin peroxidase of Phanerochaete chrysosporium. Methods of Enzymology, 161, 238–249.CrossRefGoogle Scholar
  38. Turner, E. M. (1974). Phenoloxidase activity in relation to substrate and development stage in mushroom Agaricus bisporus. Transactions of the British Mycological Society, 63(3), 541–547.CrossRefGoogle Scholar
  39. Wang, H., Brown, S. L., Magesan, G. N., Slade, A. H., Quintern, M., & Clinton, P. W. (2008). Technological options for the management of biosolids. Environmental Science & Pollution Research, 15(4), 308–317.CrossRefGoogle Scholar
  40. Wang, H. Z., He, M. C., Lin, C., Quan, X. C., Guo, W., & Yang, Z. F. (2007). Monitoring and assessment of persistent organochlorine residues in sediments from the Daliaohe river watershed, northeast of China. Environmental Monitoring and Assessment, 133(1–3), 231–242.CrossRefGoogle Scholar
  41. Zhou, X. W., Su, K. Q., & Zhang, Y. M. (2012). Applied modern biotechnology for cultivation of Ganoderma and development of their products. Applied Microbiology and Biotechnology, 93(3), 941–963.CrossRefGoogle Scholar

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© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Department of Microbiology, College of Basic Sciences and HumanitiesPunjab Agricultural UniversityLudhianaIndia
  2. 2.Department of Entomology, College of AgriculturePunjab Agricultural UniversityLudhianaIndia

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