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Assessing ambient ozone injury in olive (Olea europaea L.) plants by using the antioxidant ethylenediurea (EDU) in Saudi Arabia

  • J. M. Basahi
  • I. M. Ismail
  • N. S. Haiba
  • I. A. Hassan
  • G. Lorenzini
Article

Abstract

The antiozonant chemical, ethylenediurea (N-[2-(2-oxo-1-imidazolidinyl)ethyl]-N′-phenylurea, abbreviated as EDU), was applied as stem injections or soil drenches to 5-year-old containerized plants of olive (Olea europaea L. cultivar Kalamata) in growth chambers in order to assess its ameliorative effects against realistic ozone (O3) stress. Visible injury symptoms were reduced greatly in individuals treated with EDU, with injection applications having greater protection than soil drenches. EDU application caused increases in the measured ecophysiological parameters compared to untreated individuals. In particular, the stem injection protected plants against photosynthetic impairment (unchanged net photosynthetic rates and intercellular CO2 concentration, in comparison to plants grown in filtered air). EDU application increased the protection of PSII from ambient O3 oxidative stress, although it did not retain the proportion of redox state of QA, pigment composition of photosynthetic apparatus and size of light-harvesting complex of PSII. However, the stem injection of plants with EDU induced lower non-photochemical quenching (NPQ) values in comparison to ambient air (−2 %), indicating a better photoprotection of PSII in comparison to soil drench application. EDU application caused increases in the morphological and biometric parameters compared to individuals exposed to ambient air. To the best of our knowledge, this is the first study highlighting the protection of Kalamata olive trees due to EDU in terms of growth, yield, visible injury, and photosynthetic performance. Furthermore, this study proved that EDU could be a low-cost and a low-technology efficient tool for assessing O3 effects on plant performances in the field in Saudi Arabia.

Keywords

O3 Stem injection Soil drench Photosynthesis Growth and yield 

Notes

Acknowledgments

We would like to thank Prof. Jeremy D. Barnes (Dept. Agricultural and Environmental Science, Newcastle University, Newcastle upon Tyne, UK) for a generous supply of EDU. Thanks are also due to Elisa Pellegrini and Lorenzo Cotrozzi, University of Pisa, for their helpful contribution to discussion.

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • J. M. Basahi
    • 1
  • I. M. Ismail
    • 1
  • N. S. Haiba
    • 2
  • I. A. Hassan
    • 1
    • 3
  • G. Lorenzini
    • 4
  1. 1.Air Pollution Laboratory (APL), Centre of Excellence in Environmental Studies (CEES)King Abdulaziz UniversityJeddahSaudi Arabia
  2. 2.Department of Chemistry, Faculty of EducationAlexandria UniversityAlexandriaEgypt
  3. 3.Faculty of ScienceAlexandria UniversityAlexandriaEgypt
  4. 4.Department of Agriculture, Food and EnvironmentThe University of PisaPisaItaly

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