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A combined liquid chromatography-triple-quadrupole mass spectrometry method for the residual detection of veterinary drugs in porcine muscle, milk, and eggs

  • Dan Zhang
  • Zee-Yong Park
  • Jin-A Park
  • Seong-Kwan Kim
  • Daun Jeong
  • Sang-Hyun Cho
  • Jae-Han Shim
  • Jin-Suk Kim
  • A. M. Abd El-AtyEmail author
  • Ho-Chul ShinEmail author
Article

Abstract

A liquid chromatography-electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method was developed for monitoring and detection of four different drugs, namely acetanilide, pentylenetetrazole, phenacetin, and tetramethrin in porcine muscle, pasteurized milk, and table egg samples. For acetanilide and pentylenetetrazole, the samples were extracted with 0.1 % formic acid in acetonitrile, followed by defatting with n-hexane, partitioning at −20 °C for 1 h, centrifugation, and filtration, whereas the quick, easy, cheap, effective, rugged, and safe “QuEChERS” method was used for phenacetin and tetramethrin. The final extracts were combined and analyzed in a single chromatographic run using an XBridgeTM analytical column and 0.1 % formic acid and 10 mM ammonium formate in ultrapure water (A) and 0.1 % formic acid and 10 mM ammonium formate in methanol (B) as the mobile phase. Owing to the unavailability of internal standards, matrix-matched calibrations were used for analyte quantification with coefficients of determination (R 2) ≥ 0.9865. The intra- and inter-day accuracies ranged from 60.75 to 90.90 % and from 63.75 to 89.30 %, respectively, while the respective analytical precisions were 1.48–17.44 % (23.3 % for porcine sample spiked with phenacetin) and 1.97–15.78 %. The limits of quantification (LOQ) were between 0.5 and 2.5 ng/g in the matrices tested. Food samples purchased from local markets in Seoul were analyzed using the developed method and none of the tested drugs was detected.

Keywords

Veterinary drugs Animal food products QuEChERS Tandem mass spectrometry Porcine muscle Milk Table eggs 

Notes

Acknowledgments

This study was supported by a grant (14162MFDS886) from the Ministry of Food and Drug Safety Administration in 2014.

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Dan Zhang
    • 1
  • Zee-Yong Park
    • 2
  • Jin-A Park
    • 1
  • Seong-Kwan Kim
    • 1
  • Daun Jeong
    • 1
  • Sang-Hyun Cho
    • 1
  • Jae-Han Shim
    • 3
  • Jin-Suk Kim
    • 1
  • A. M. Abd El-Aty
    • 1
    • 4
    Email author
  • Ho-Chul Shin
    • 1
    Email author
  1. 1.Department of Veterinary Pharmacology and Toxicology, College of Veterinary MedicineKonkuk UniversitySeoulRepublic of Korea
  2. 2.School of Life SciencesGwangju Institute of Science and TechnologyGwangjuRepublic of Korea
  3. 3.Biotechnology Research Institute, College of Agriculture and Life SciencesChonnam National UniversityGwangjuRepublic of Korea
  4. 4.Department of Pharmacology, Faculty of Veterinary MedicineCairo UniversityGizaEgypt

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