Environmental Monitoring and Assessment

, Volume 186, Issue 5, pp 3277–3282 | Cite as

Validation of spectrophotometric method for Se(IV) determination: analytical applications

  • Gladiola Tantaru
  • Madalina Vieriu
  • Maria-Cristina Popescu
Article
First Online:
Received:
Accepted:
  • 407 Downloads

Abstract

As selenium is an important part of the antioxidant enzymes and also because there are several studies suggesting a possible link between cancer and selenium deficiency, this paper presents a spectrophotometric method for the assay of Se(IV), using N,N-diethyl-p-phenylenediamine monohydrochloride as reagent. The proposed method is based on the reaction between the selenium and potassium iodide in low acidic medium, when iodine is released. This last product will further oxidise the new reagent. The final obtained product is strongly coloured in red and has an absorption maximum at 552 nm and molar extinction coefficient (ε) of 6.1 × 104 L mol−1 cm−1. The optimum working conditions were established, and the developed method was validated, being characterised by a good linearity (in the range of 0.5–3.0 μg/mL), a limit of detection (0.0573 μg/mL) and a limit of quantification (0.1737 μg/mL). At the same time, the repeatability, the precision of the method and the accuracy were established. The proposed and validated method was applied with good results for the determination of Se(IV) in spring and bottled water from Iasi and also in pharmaceutical and cosmetic products.

Keywords

Selenium Spectrophotometric method Validation 
This is a preview of subscription content, log in to check access.

References

  1. Beck, M. A., Levander, O., & Handy, J. (2003). Selenium deficiency and viral infection. Journal of Nutrition, 133, 1463S–1467S.Google Scholar
  2. Combs, G. F., Clark, L. C., & Turnbull, B. W. (2001). An analysis of cancer prevention by selenium. BioFactors, 14, 153–159.CrossRefGoogle Scholar
  3. Food and Nutrition Board, Institute of Medicine. (2000). Dietary reference intakes for vitamin C, vitamin E, selenium and carcinoids (pp. 284–324). New York: National Academic.Google Scholar
  4. Goldhaber, S. B. (2003). Trace element risk assessment: essentiality vs. toxicity. Regulatory Toxicology and Pharmacology, 38, 232–242.CrossRefGoogle Scholar
  5. Hathcock, J. (1997). Vitamins and minerals: efficacy and safety. American Journal of Clinical Nutrition, 66, 427–437.Google Scholar
  6. Knekt, P., Marniemi, J., Teppo, L., Heliovaara, M., & Aromaa, A. (1998). Is low selenium status a risk factor for lung cancer? American Journal of Epidemiology, 148, 975–982.CrossRefGoogle Scholar
  7. Kose, K., Dogan, P., Kardas, Y., & Saraymen, R. (1996). Plasma selenium levels in rheumatoid arthritis. Biological Trace Element Research, 53, 51–56.CrossRefGoogle Scholar
  8. Levander, O. A., & Beck, M. A. (1997). Interacting nutritional and infectious etiologies of Keshan disease. Insights from coxsackie virus B-induced myocarditis in mice deficient in selenium or vitamin E. Biological Trace Element Research, 56, 5–21.CrossRefGoogle Scholar
  9. Lippman, S. M., Klein, E. A., Goodman, P. J., Lucia, M. S., Thompson, I. M., Ford, L. G., et al. (2009). Effect of selenium and vitamin E on risk of prostate cancer and other cancers: the Selenium and Vitamin E Cancer Prevention Trial (SELECT). Journal of American Medical Association, 301, 39–51.CrossRefGoogle Scholar
  10. Neve, J. (1996). Selenium as risk factors for cardiovascular disease. Journal of Cardiovascular Risk, 3, 42–47.CrossRefGoogle Scholar
  11. Niedzielski, P., & Siepak, M. (2003). Analytical methods for determining arsenic, antimony and selenium in environmental samples. Polish Journal of Environmental Studies, 12(6), 653–667.Google Scholar
  12. Revanasiddappa, H. D., & Kiran Kumar, T. N. (2001). A facile spectrophotometric method for the determination of selenium. Analytical Science, 17, 1309–1312.CrossRefGoogle Scholar
  13. Sasakura, C., & Suzuki, K. T. (1998). Biological interaction between transition metals (Ag, Cd and Hg), selenide/sulfide and selenoprotein P. Journal of Inorganic Biochemistry, 71, 159–162.CrossRefGoogle Scholar
  14. Singhal, N., & Austin, J. (2002). A clinical review of micronutrients in HIV infection. International Association of Physicians in AIDS Care, 1, 63–75.CrossRefGoogle Scholar
  15. Terry, N., Zayed, A. M., DeSouza, M. P., & Tarun, A. N. (2000). Selenium in higher plants. Annual Review of Plant Physiology and Plant Molecular Biology, 51, 401–432.CrossRefGoogle Scholar
  16. Whange, P. D. (2002). Selenocompounds in plants and animals and their biological significance. Journal of the American College of Nutrition, 21(3), 223–232.CrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Gladiola Tantaru
    • 1
  • Madalina Vieriu
    • 1
  • Maria-Cristina Popescu
    • 2
  1. 1.Faculty of Pharmacy“Grigore T. Popa” University of Medicine and PharmacyIasiRomania
  2. 2.“P. Poni” Institute of Macromolecular Chemistry of Romanian AcademyIasiRomania

Personalised recommendations