Environmental Monitoring and Assessment

, Volume 184, Issue 10, pp 6025–6036 | Cite as

Monitoring of marine mucilage formation in Italian seas investigated by infrared spectroscopy and independent component analysis

  • Mauro Mecozzi
  • Marco Pietroletti
  • Michele Scarpiniti
  • Rita Acquistucci
  • Marcelo Enrique Conti
Article

Abstract

The aim of this study is to present and to discuss some characteristics of recalcitrant organic matter mechanism and formation. These aggregates called mucilages that are produced by the degradation reactions of several algae, have been investigated by infrared (FTIR) spectroscopy. FTIR spectra of macroaggregates produced by different algal samples have been daily collected in order to investigate the steps of aggregation. Afterwards, they have been elaborated by means of Independent Component Analysis (ICA). ICA investigation of FTIR spectra showed that the global aggregation process of marine mucilage always consisted of two different phases or independent components (ICs). One IC is related to the first degradation step of algal cells leading to the production of mono and oligosaccharides with aminoacids and oligopeptides. The second IC is related to the polymerization of oligosaccharides with aminoacids and oligopeptides and to their interaction with less polar compounds such as lipids thus producing supramolecular structures. The emerging mechanisms of anomalous size aggregates of organic matter match those of natural organic matter aggregation. The approach we suggest is to use synthetic mucilages which allows to monitor the macroaggregates formation because it can hardly be performed by means of natural marine macroaggregates.

Keywords

Marine mucilage Humification Independent component analysis 

References

  1. Alvarez-Puebla, R. A., & Garrido, J. J. (2005). Effect of pH on the aggregation of a gray humic acid in colloidal and solid states. Chemosphere, 59, 659–667.CrossRefGoogle Scholar
  2. Buzzelli, E., Gianna, R., Marchiori, E., & Bruno, M. (1997). Influence of nutrient factors on production of mucilage by Amphora coffeaeformis var. perpusilla. Continental Shelf Research, 17, 1171–1180.CrossRefGoogle Scholar
  3. Choi, S., Cichocki, A., Park, H., & Lee, S. (2005). Blind source separation and independent component analysis: a review. Neural Inference Processing—Letters and Reviews, 6, 1–57.Google Scholar
  4. Chun Chin, W., Orellana, M., & Verdugo, P. (1998). Spontaneous assembly of marine organic matter into polymer gels. Nature, 391, 468–472.CrossRefGoogle Scholar
  5. Conti, M. E. (1996). The pollution of the Adriatic Sea: scientific knowledge and policy actions. International Journal of Environment and Pollution, 6, 113–130.Google Scholar
  6. Conti, M. E., Iacobucci, M., & Cecchetti, G. (2005). A statistical approach applied to trace metal data from biomonitoring studies. International Journal of Environment and Pollution, 23, 29–41.CrossRefGoogle Scholar
  7. Conti, M. E., Iacobucci, M., & Cecchetti, G. (2007). A biomonitoring study: trace metals in seagrass, algae and molluscs in a marine reference ecosystem (Southern Tyrrhenian Sea). International Journal of Environment and Pollution, 29, 308–332.CrossRefGoogle Scholar
  8. Conti, M. E., Bocca, B., Iacobucci, M., Finoia, M. G., Mecozzi, M., Pino, A., et al. (2010). Baseline trace metals in seagrass, algae and molluscs in a southern Tyrrhenian ecosystem (Linosa Island, Sicily). Archives of Environmental Contamination and Toxicology, 58, 79–95.CrossRefGoogle Scholar
  9. De Angelis, F., Barbarulo, M. V., Bruno, M., Volterra, L., & Nicoletti, R. (1993). Chemical composition and biological origin of dirty sea mucilages. Phytochemistry, 34, 393–395.CrossRefGoogle Scholar
  10. Deserti, M., Cacciamani, C., Chiggiato, J., Rinaldi, A., & Ferrari, C. R. (2005). Relationships between northern Adriatic Sea mucilage events and climate variability. Science of the Total Environment, 353, 82–88.CrossRefGoogle Scholar
  11. Ding, Y., Chin, W., Rodriguez, A., Hung, C., Santschi, P. H., & Verdugo, P. (2008). Amphiphilic exopolymers from Sagittula stellata induce DOM self-assembly and formation of marine microgels. Marine Chemistry, 112, 11–19.Google Scholar
  12. Grilli, F., Paschini, E., Precali, R., Russo, A., & Supić, N. (2005a). Circulation and horizontal fluxes in the Northern Adriatic Sea in the period June 1999–July 2002. Part I: geostrophic circulation and current measurements. Science of the Total Environment, 353, 57–67.CrossRefGoogle Scholar
  13. Grilli, F., Marini, M., Degobbis, D., Ferrari, C. R., Fornasiero, P., Russo, A., et al. (2005). Circulation and horizontal fluxes in the northern Adriatic Sea in the period June 1999–July 2002, Part II: nutrients transport. Science of the Total Environment, 353, 115–125.CrossRefGoogle Scholar
  14. Guetzeloff, T. J., & Rice, J. A. (1996). In J. S. Gaffey, S. A. Marley, & S. B. Clark (Eds.), Micelle nature of humic colloids. Washington DC: American Chemical Society.Google Scholar
  15. Guibaud, G., Tixier, N., Bouju, A., & Baudu, M. (2003). Relation between extracellular polymers' composition and its ability to complex Cd, Cu and Pb. Chemosphere, 52, 1701–1710.CrossRefGoogle Scholar
  16. Guibaud, G., Comte, S., Bordas, F., Dupuy, S., & Baudu, M. (2005). Comparison of the complexation of polymeric substances (EPS), extracted from activated sludges and produced by pure bacteria strains, for cadmium, lead and nickel. Chemosphere, 59, 629–638.CrossRefGoogle Scholar
  17. Guibaud, G., van Hullebusch, E., & Bordas, F. (2006). Lead and cadmium biosorption by extracellular polymeric substances (EPS) extracted from activated sludges: pH-sorption edge tests and mathematical equilibrium modelling 64, 1955–1962.Google Scholar
  18. Hyvarinen, A. (1999). Fast and robust fixed-point algorithms for independent component analysis. IEEE Transactions on Neural Networks, 10, 626–634.CrossRefGoogle Scholar
  19. Hyvarinen, A., & Oja, E. (1997). A fast fixed-point algorithm for independent component analysis. Neural Computation, 9, 1483–1492.CrossRefGoogle Scholar
  20. Hyvärinen, A., & Oja, E. (2000). Independent component analysis: algorithms and applications. Neural Networks, 13, 411–430.CrossRefGoogle Scholar
  21. Hyvärinen, A., Karhunen, J., & Oja, E. (2001). Independent component analysis. New York, USA: John Wiley & Sons.CrossRefGoogle Scholar
  22. Innamorati, M. (1995). Hyperproduction of mucilages by micro and macroalgae in the Tyrrhenian sea. Science of the Total Environment, 165, 65–81.CrossRefGoogle Scholar
  23. Ishiwatari, R. R. (1992). Macromolecular materials (humic substances) in the water column and sediments. Marine Chemistry, 39, 151–166.CrossRefGoogle Scholar
  24. Jung, W., Choi, I., Oh, S., Park, S., Seo, S., Lee, S., et al. (2009). Anti-asthmatic effect of marine red alga (Laurencia undulata) polyphenolic extracts in a murine model of asthma. Food and Chemical Toxicology, 47, 293–297.CrossRefGoogle Scholar
  25. Kokalj, M., Metka Rihtarič, M., & Kreft, S. (2011). Commonly applied smoothing of IR spectra showed unappropriate for the identification of plant leaf samples. Chemometrics and Intelligent Laboratory Systems, 108, 154–161.CrossRefGoogle Scholar
  26. Leppard, G. G. (1995). The characterization of algal and microbial mucilages and their aggregates in aquatic ecosystems. Science of the Total Environment, 165, 103–131.CrossRefGoogle Scholar
  27. Maie, N., Pisani, O., & Jaffè, R. (2008). Mangrove tannins in aquatic ecosystems: their fate and possible influence on dissolved organic carbon and nitrogen. Limnology and Oceanography, 53, 160–171.CrossRefGoogle Scholar
  28. Marche, T., Schnitze, M., Dinel, H., Paré, T., Champagne, P., Schulten, H.-R., et al. (2003). Chemical changes during composting of a paper mill sludge–hardwood sawdust mixture. Geoderma, 116, 345–356.CrossRefGoogle Scholar
  29. Mecozzi, M., & Pietrantonio, E. (2006). Carbohydrates proteins and lipids in fulvic and humic acids of sediments and its relationships with mucilaginous aggregates in the Italian seas. Marine Chemistry, 101, 27–39.CrossRefGoogle Scholar
  30. Mecozzi, M., Acquistucci, R., Di Noto, V., Pietrantonio, E., Amici, M., & Cardarilli, D. (2001). Characterization of mucilage aggregates in Adriatic and Tyrrhenian sea: structure similarities between mucilage samples and the insoluble fractions of marine humic substance. Chemosphere, 44, 711–722.CrossRefGoogle Scholar
  31. Mecozzi, M., Pietrantonio, E., Di Noto, V., & Papái, Z. (2005). The humin structure of mucilage aggregates from the Adriatic and Tyrrhenian sea: a reasonable cause of mucilage formation. Marine Chemistry, 95, 255–269.CrossRefGoogle Scholar
  32. Mecozzi, M., Pietroletti, M., & Conti, M. E. (2008). The complex mechanisms of marine mucilage formation by spectroscopic investigation of the structural characteristics of natural and synthetic mucilage samples. Marine Chemistry, 112, 38–52.CrossRefGoogle Scholar
  33. Mecozzi, M., Pietroletti, M., Gallo, V., & Conti, M. E. (2009). Formation of incubated marine mucilages investigated by FTIR and UV–VIS spectroscopy and supported by two-dimensional correlation analysis. Marine Chemistry, 116, 18–35.CrossRefGoogle Scholar
  34. Monakhova, Y. B., Astakhov, S. A., Kraskov, A., & Mushtakova, S. P. (2010). Independent components in spectroscopic analysis of complex mixtures. Chemometrics and Intelligent Laboratory System, 103, 108–115.CrossRefGoogle Scholar
  35. Monti, M., Welker, C., Dellavalle, G., Casaretto, L., & Fonda Umani, S. (1995). Mucous aggregates under natural and laboratory conditions: a review. Science of the Total Environment, 165, 145–154.CrossRefGoogle Scholar
  36. Orellana, M. V., Peterasen, T. W., Diercks, A. H., Donohoe, S., Verdugo, P., & van den Engh, G. (2007). Marine microgels: optical and proteomic fingerprints. Marine Chemistry, 105, 229–239.CrossRefGoogle Scholar
  37. Piccolo, A. (2001). The supramolecular structure of humic substance. Soil Science, 166, 810–832.CrossRefGoogle Scholar
  38. Piccolo, A., Conte, P., & Cozzolino, A. (2001). Chromatographic and spectrophotometric properties of dissolved humic substances compared with macromolecular polymers. Soil Science, 166, 174–185.CrossRefGoogle Scholar
  39. Pistocchi, R., Trigari, G., Serrazanetti, G. P., Taddei, P., Monti, G., Palamidesi, S., et al. (2005). Chemical and biochemical parameters of cultured diatoms and bacteria from the Adriatic sea as possible biomarkers of mucilage production. Science of the Total Environment, 353, 287–299.CrossRefGoogle Scholar
  40. Polvillo, O., González-Pérez, J. A., Boski, T., & González-González-Vila, F. J. (2009). Structural features of humic acids from a sedimentary sequence in the Guadiana estuary (Portugal-Spain border). Organic Geochemistry, 40, 20–28.CrossRefGoogle Scholar
  41. Savitzky, A., & Golay, M. J. E. (1964). Smoothing and differentiation of data by simplified least squares procedures. Analytical Chemistry, 36, 1627–1639.CrossRefGoogle Scholar
  42. Schulten, H. R., & Schnitzer, M. (1995). Three dimensional models for humic acids and soil organic matter. Naturwissenschaften, 82, 487–498.CrossRefGoogle Scholar
  43. Schwanninger, M., Hinterstoisser, B., Gradiger, C., Mesner, K., & Fackler, K. (2004). Examination of spruce wood biodegraded by Ceriporiopsis subvermispora using near and mid infrared spectroscopy. Journal of Near Infrared Spectroscopy, 12, 397–409.CrossRefGoogle Scholar
  44. Shank, C. G., Lee, R., Vähätalo, A., Zepp, R. G., & Bartels, E. (2010). Production of chromophoric dissolved organic matter from mangrove leaf litter and floating Sargassum colonies. Marine Chemistry, 119, 172–181.CrossRefGoogle Scholar
  45. Skoog, A., Alldredge, A., Passow, U., Dunne, J., & Murray, J. (2008). Neutral aldoses as source indicators for marine snow. Marine Chemistry, 108, 195–206.CrossRefGoogle Scholar
  46. Verdugo, P., Alldredge, A. L., Azam, F., Kirchman, D. L., Passow, U., & Santschi, P. H. (2004). The oceanic gel phase: a bridge in the DOMPOM continuum. Marine Chemistry, 92, 67–85.CrossRefGoogle Scholar
  47. Vollenweider, R. A., & Rinaldi, A. (1995). Editorial. Science of the Total Environment, 165, 5–7.CrossRefGoogle Scholar
  48. Volterra, L., & Conti, M. E. (2000). Algae as biomarkers, bioaccumulators and toxin producers. In Conti M. E., Botre` F. (Eds.) The Control of Marine Pollution: Current Status and Future Trends. International Journal of Environment and Pollution, 13, 92–125.Google Scholar
  49. Von Wandruska, R. (2000). Humic acids: their detergent qualities and potential uses in pollution remediation. Geochemical Transations, 1, 10.CrossRefGoogle Scholar
  50. Wang, G., Ding, Q., & Hou, Z. (2008). Independent component analysis and its applications in signal processing for analytical chemistry. Trends in Analytical Chemistry, 27, 368–376.CrossRefGoogle Scholar
  51. Wells, M. L., & Goldberg, E. D. (1993). Colloids aggregation in seawater. Maine Chemistry, 41, 353–358.Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Mauro Mecozzi
    • 1
  • Marco Pietroletti
    • 1
  • Michele Scarpiniti
    • 2
  • Rita Acquistucci
    • 3
  • Marcelo Enrique Conti
    • 4
  1. 1.Laboratory of Chemometrics and Environmental Applications, ISPRARomeItaly
  2. 2.INFOCOM Department‘Sapienza’ University of RomeRomeItaly
  3. 3.INRAN, National Institute for Research on Food and NutritionRomeItaly
  4. 4.Department of ManagementUniversity of RomeRomeItaly

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