Environmental Monitoring and Assessment

, Volume 130, Issue 1–3, pp 215–220 | Cite as

Serogroups of Escherichia coli from Drinking Water

Original Article

Abstract

Fifty seven isolates of thermotolerant E. coli were recovered from 188 drinking water sources, 45 (78.9%) were typable of which 15 (26.3%) were pathogenic serotypes. Pathogenic serogroup obtained were 04 (Uropathogenic E. coli, UPEC), 025 (Enterotoxigenic E. coli, ETEC), 086 (Enteropathogenic E. coli, EPEC), 0103 (Shiga-toxin producing E. coli, STEC), 0157 (Shiga-toxin producing E. coli, STEC), 08 (Enterotoxigenic E. coli, ETEC) and 0113 (Shiga-toxin producing E. coli, STEC). All the pathogenic serotypes showed resistance to bacitracin and multiple heavy metal ions. Resistance to streptomycin and co-trimazole was detected in two strains whereas resistance to cephaloridine, polymixin-B and ampicillin was detected in one strain each. Transfer of resistances to drugs and metallic ions was observed in 9 out of 12 strains studied. Resistances to bacitracin were transferred in all nine strains. Among heavy metals resistance to As3+ followed by Cr6+ were transferred more frequently.

Keywords

Thermotolerant E. coli UPEC ETEC EPEC STEC Antibiotic resistance 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. APHA. (1992). Standard methods for the examination of water and wastewater (18th ed.). Washington, D.C.: American Public Health Association.Google Scholar
  2. Bauer, A. W., Kirby, W. M. M., Sherris, J. C., & Twick, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45, 493.Google Scholar
  3. Bolton, F. J., Crozier, L., & Williamson, J. K. (1996). Isolation of E. coli 0157 from raw meat products. Letters in Applied Microbial, 23, 317–321.Google Scholar
  4. Bonnet, R., Souweine, B., Gauthier, G., Rich, C., Livrelli, V., Sirot, J., et al. (1998). Non-0157:H 7 Stx2-producing E. coli strain associated with sporadic cases of hemolytic–uremic syndrome in adults. Journal of Clinical Microbiology, 36, 1777–1780.Google Scholar
  5. Boyce, T. G., Swerdlow, D. L., & Griffin, P. M. (1995). Current concepts: E. coli 0157:H7 and the hemotypic uremic syndrome. New England Journal of Medicine, 333, 364.CrossRefGoogle Scholar
  6. Cervantes-vega, C., Chanez, J., Cordona, N. A., de la More, P., & Velasco, J. A. (1986). Resistance to metals by Pseudomonas aeruginosa clinical isolates. Microbios, 48, 159.Google Scholar
  7. Chattopadhya, D., & Basu, S. (1986). Modified scheme for identification of coliform organism in drinking water. Indian Journal of Medical Research, 83, 152.Google Scholar
  8. Day, N. P., Scotland, S. M., Cheasty, T., & Rowe, B. (1983). E. coli 0157: H7 associated with human infections in the United Kingdom. Lancet, 1, 825.CrossRefGoogle Scholar
  9. Griffin, P. M. (1999). Escherichia coli 0157: H7 and other enterohaemorrhagic E. coli. In M. J. Blaser, P. D. Smith, J. I. Ravdin, H. B. Greenberg & R. L. Guerrant (Eds.), Infections of the gastrointestinal Tract I (p. 739). New York: Raven.Google Scholar
  10. Kaper, J. B. (1994). Molecular pathogenesis of enteropathogenic Escherichia coli. In V. L. Miller, J. B. Kaper, D. A. Portnoy & R. R. Iceberg (Eds.), Molecular genetics of bacterial pathogenesis (p. 173). Washington: ASM.Google Scholar
  11. Kita, E., Yunou, Y., Kurioka, T., Harada, H., Yoshikawa, S., Mikasa, K., et al. (2000). Pathogenic mechanism of mouse brain damage caused by oral infection with shiga toxin producing Escherichia coli 0157: H7. Infection and Immunity, 68, 1207.CrossRefGoogle Scholar
  12. Lindenthal, C., & Elinghorst, E. A. (2001). Enterotoxigenic Escherichia coli TibA glycoprotein adheres to human intestine epithelial cells. Infection and Immunity, 69, 52.CrossRefGoogle Scholar
  13. Maniatis, T., Fritsch, E. F., & Sambrook, J. (1982). Molecular cloning, a laboratory manual. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory.Google Scholar
  14. Meyers, J. A., Sanchez, D., Elwell, L. P., & Falkow, S. (1976). Simple agarose gel electrophoretic methods for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology, 127, 1529.Google Scholar
  15. Muhldorfer, I., & Hacker, J. (1994). Genetic aspect of E. coli virulence. Microbial Pathogenesis, 16, 171.CrossRefGoogle Scholar
  16. Nataro, J. P., & Kapar, J. B. (1998). Diarrheagenic Escherichia coli. Clinical Microbiology Reviews, 11, 142.Google Scholar
  17. Oswald, E., Schmidt, H., Moradito, S., Karch, H., Marches, O., & Caprioli, A. (2000). Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: Characterization of a new intimin variant. Infection and Immunity, 68, 64.CrossRefGoogle Scholar
  18. Pichel, M., Binsztein, N., & Viboud, G. (2000). Cs22, a novel human enteroxigenic Escherichia coli adhesin, is related to Cs15. Infection and Immunity, 68, 3280.CrossRefGoogle Scholar
  19. Ramteke, P. W. (1995). Comparison of standard most probable number method with three alternate tests for detection of bacteriological water quality indicators. Environmental Toxicology and Water Quality, 10, 173–178.CrossRefGoogle Scholar
  20. Ramteke, P. W., Bhattacharjee, J. W., Pathak, S. P., & Kalra, N. (1992). Evaluation of coliforms as indicators of water quality in India. Journal of Applied Bacteriology, 72, 352–356.Google Scholar
  21. Tewari, S., Ramteke, P. W., & Garg, S. K. (2003). Evaluation of simple microbial tests for detection of fecal coliforms directly at 45.5°C. Environmental Monitoring and Assessment, 85, 191–198.CrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media B.V. 2006

Authors and Affiliations

  1. 1.Department of BiotechnologyAllahabad Agricultural Institute (Deemed University)AllahabadIndia

Personalised recommendations