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Environmental Monitoring and Assessment

, Volume 119, Issue 1–3, pp 1–13 | Cite as

Evaluation of the Fertilization Capability of Paracentrotus Lividus Sea Urchin Storaged Gametes by the Exposure to Different Aqueous Matrices

  • S. LeraEmail author
  • D. Pellegrini
Article

Abstract

Several experiments were performed to evaluate the fertilization capability of Paracentrotus lividus sea urchin gametes, which are usually used in fertilization tests, but which, until now, were employed only for few hours after spawning.

The aim of this work is to evaluate the possibility of performing the bioassay for longer periods, employing ‘conserved' gametes. As far as the method, the sperm was stored ‘dry' at 4 C; eggs were conserved in an antibiotic solution at 4 C, and they were exposed both to copper solutions as reference toxicant and to aqueous matrices.

The use of conserved gametes in the fertilization bioassay lead to EC50 values which were acceptable for about 3 days. Moreover, these experiments permitted to identify the limiting factor: the male gamete.

Keywords

antibiotics bioassay copper gametes storage Paracentrotus lividus sea urchin aqueous matrices 

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References

  1. Anderson, S., Hoffman, E., Steward, D. and Harte, J.: 1990, Ambient Toxicity Characterization of San Francisco Bay and Adjacent Wetland Ecosystem, Report, LBL-29579, Lawrence Berkeley Laboratory, University of California, pp. 1–85.Google Scholar
  2. Ankley, G. T.: 1991, ‘Predicting the toxicity of bulk sediments to aquatic organisms with aqueous test fractions: Pore water vs. Elutriate’, Environmental Toxicology and Chemistry 10, 1359–1366.Google Scholar
  3. Arizzi Novelli, A., Volpi Ghirardini, A., Giuliani, S., Falugi, C., Pagano, G. and Iaccarino, M.: 2001, ‘Valutazione della tossicità di acque e sedimenti costieri con gameti e embrioni di specie autoctone di ricci di mare utilizzando metodiche differenziate’, Biol. Mar. Medit. 8 (2), 41–59.Google Scholar
  4. ASTM: 1995, ‘Standard guide for conducting static acute toxicity tests with echinoid embryos’, E 1563-95, American Society for Testing and Materials, Philadelphia, pp. 1029–1046.Google Scholar
  5. Carr, R. S. and Chapman, D. C.: 1995, ‘Comparison of methods for conducting marine and estuarine sediment porewater toxicity tests: Extraction, storage and handling techniques’, Arch. Environ. Contam. Toxicol. 28, 69–77.CrossRefGoogle Scholar
  6. Chapman, G. A.: 1995, ‘Sea urchin sperm cell test’, in: G.M. Rand (ed.) Fundamentals of Aquatic Toxicology: Effects, Environmental Fate and Risk Assessment, 2nd Edition, Bristol, PA, Taylor and Francis, pp. 189–205.Google Scholar
  7. Cherr, G. N., Shenker, J. M., Lundmark, C. and Turner, K. O.: 1987, ‘Toxic effects of selected bleached kraft mill effluent constituents on the sea urchin sperm cell’, Toxicol. Chem. 6, 561–569.Google Scholar
  8. Clementson, L. A. and Wayte, S. E.: 1992, ‘The effects of frozen storage of open-ocean seawater samples on the concentration of dissolved Phosphate and Nitrate’, Wat. Res. 26 (9), 1171–1176.CrossRefGoogle Scholar
  9. De Angelis, E.: 1975, ‘The problem of bacterial contamination during sea urchin egg culture’, Pubbl. Staz. Zool. 40, 19–25.Google Scholar
  10. De Angelis, E.: 1976, ‘The effects of antibiotics on sea urchin egg culture’, Pubbl. Staz. Zool. 40, 26–33.Google Scholar
  11. Decreto legislativo no. 152: 1999, ‘Disposizione sulla tutela delle acque dell'inquinamento e recepimento della direttiva 91/271/CEE concernente il trattamento delle acque reflue e della direttiva 91/676/CEE relativa alla protezione delle acque dell'inquinamento provocato dai nitrati provenienti da fonti agricole’, Gazzetta Ufficiale, 29 Maggio 1999, pp. 1–125.Google Scholar
  12. Dinnel, P. A., Link, J. M. and Stober, Q. J.: 1987, ‘Improved methodology for a sea urchin sperm cell bioassay for marine waters’, Arch. Environ. Contam. Toxicol 16, 23–32.CrossRefGoogle Scholar
  13. Dinnel, P. A., Pagano, G. and Ossida, P. S.: 1988, ‘A sea urchin test system for marine environmental monitoring’, in: R. D. Burke, P. V. Mladenov and R. L. Parsley (eds.), Echinoderm Biology. Proceedings of the Sixth International Echinoderm Conference, Balkema, Rotterdam, pp. 611–619.Google Scholar
  14. Fenaux, L.: 1968, ‘Maturation des gonads et cycle saissonier des larves chez Arbacia lixula, Paracentrotus lividus, et Psammechinus microtuberculatus (Echinedes) à Villefranche-sur-Mer’, Vie Milieu 19, 1–52.Google Scholar
  15. Finney, D. J.: 1971, Probit Analysis, Cambridge University Press, London, UK.Google Scholar
  16. Giambartolomei, F. M.: 1990, ‘Nuove acquisizioni sul test di fertilità dello sperma di riccio di mare Paracentrotus lividus’, Thesis, University of Padova, Italy.Google Scholar
  17. Giuliani, S., Ennas, C., Pellegrini, D. and Kozinkova, L.: 2002, ‘The bioassay with sea urchin Paracentrotus lividus: Different end-points to be applied to different environmental studies’, Fres. Environ. Bull. 11 (10), 806–809.Google Scholar
  18. Giuliani, S., Lera, S., Kozinkova, L., De Ranieri, S. and Pellegrini, D.: 2004, ‘Sensibilità del saggio di fecondazione con gameti di Paracentrotus lividus (Lamarck) al variare di alcune condizioni sperimentali’, Biol. Mar. Medit., in press.Google Scholar
  19. Hamilton, M. A., Russo, R. C. and Thurston, R. V.: 1978, ‘Trimmed Spearman-Karber method for estimating median lethal concentrations in toxicity bioassays’, Environ. Sci. Technol. 12, 714–720.CrossRefGoogle Scholar
  20. ICES: 1997, Report of the ICES Advisory Committee on the Marine Environment.Google Scholar
  21. Kobayashi, N.: 1984, ‘Marine ecotoxicological testing with echinoderms’, in: G. Persoone, E. Jaspers and C. Claus (eds.), Ecotoxicological Testing for the Marine Environment, State Univ. Ghent and Inst. Mar. Scient. Res., Bredene, Belgium, pp. 341–405.Google Scholar
  22. Kozinkova, L., Ennas, C., Giuliani, S. and Pellegrini, D.: 2003, ‘Valutazione della qualità dei gameti di Paracentrotus lividus (Lamarck) utilizzando Cu(NO3)2·3H2O come tossico di riferimento su animali stabulati e non stabulati’, Biol. Mar. Medit. 10 (2), 1101–1104.Google Scholar
  23. Lambertson, J. Q., Dewitt, T. H. and Swartz, C.: 1992, ‘Assessment of sediment toxicity to marine benthos’, in: G. A. Burton (ed.), Lewis Publishers, Sediment Toxicity Assessment, pp. 183–211.Google Scholar
  24. Mackintosh, R. F. and Bell, E.: 1970, ‘Reversible response to puromycin and some characteristics of the uptake and use of aminoacids by unfertilized sea urchin eggs’, Biol. Bull. 139, 296–303.Google Scholar
  25. Marin, M. G., Da Los, L., Moschino, V. and Campesan, G.: 2001, ‘Sediment elutriate toxicity testing with embryos of sea urchin (Paracentrotus lividus)’, Aquatic Ecosystem Health and Management 4, 215–221.CrossRefGoogle Scholar
  26. Nemer, M. and Infante, A.: 1967, ‘Ribosomal ribonucleic acid of the sea urchin egg and its fate during embryogenesis’, J. Mol. Biol. 27, 73–86.CrossRefGoogle Scholar
  27. Pagano, G., Cipollaro, M., Corsale, G., Esposito, A., Ragucci, E., Giordano, G. G. and Trieff, N. M.: 1986, ‘The sea urchin: Bioassay for the assessment of damage from environmental contaminants’, in: J. Cairns (ed.), Community Toxicity Testing, ASTM STP 920, Philadelphia, PA, pp. 66–92.Google Scholar
  28. Pellegrini, D., Giuliani, S., Onorati, F., Bigongiari, N., De Ranieri, S. and Meschini, P.: 1997, ‘Impiego di due saggi biologici per la valutazione della tossicità di sedimenti del porto di Piombino’, Biol. Mar. Medit. 4 (1), 490–492.Google Scholar
  29. Pinto, B., Pellegrini, D., Gabellini, M. and Ausili, A.: 1995, ‘Harbour and coastal sediments chemistry and toxicology: a preliminary study in dredging activities’, J. Aquat. Ecosyst. Health 4, 1–17.CrossRefGoogle Scholar
  30. Tyler, A.: 1949, ‘A simple non-injurious method for inducing repeated spawning of sea urchins and sand dollars’, Collecting Net 19, 19–20.Google Scholar
  31. U.S EPA:1991, Earl-Standard Operating Procedure Conducting the Sea Urchin Arbacia punctulata Fertilization Test, Environmental Research Laboratory, Narragansett, RI, pp. 125–131.Google Scholar
  32. U.S. EPA: 1994, Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms, 600-4-91-003, Cincinnati, Ohio.Google Scholar
  33. U.S. EPA: 1995, Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Water to West Coast Marine and Estuarine Organisms, 600/R-95/136, Cincinnati, Ohio.Google Scholar
  34. Volpi Ghirardini, A. and Arizzi Novelli, A.: 2001, ‘A sperm cell toxicity test procedure for the Mediterranean species Paracentrotus lividus (Echinodermata: Echinoidea)’, Environmental Technology 22, 439–445.CrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  1. 1.ICRAMCentral Institute of Marine ResearchRomeItaly

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