Environmental Monitoring and Assessment

, Volume 104, Issue 1–3, pp 437–444 | Cite as

Nickel and Cadmium Concentrations in Plasma and Na+/K+ATPase Activities in Erythrocyte Membranes of the People Exposed to Cement Dust Emissions

  • Temİr Alİ Demİr
  • Tamer Akar
  • Fahrettİn AkyÜz
  • Burhanettİn IŞikli
  • GÜngÖr Kanbak


The aim of this study was to determine the concentrations of nickel and cadmium in blood plasma of the people exposed to cement dust emissions and to investigate the effects of exposure period on the activities of Na+/K+ ATPase enzymes in their erythrocyte membranes. The study was carried out on people living in Eskişehir Çukurhisar rural area, located near a cement factory. Blood samples of the individuals residing in this area were taken from 80subjects (30 for control) following a physical examination. The analysis of plasma samples showed that nickel concentrations in subject group were found to be significantly higher than those of the control group (p < 0.001). Cadmium concentrations were found to be within the reference values for both group and no difference was found between the subjects and controls (p > 0.05). Furthermore, no correlation was observed between the levels of Na+/K+ ATPase activity in erythrocyte membranes of the subject group and the ages of people living in the region (p > 0.05, r = 0.133). It was also observed that nickel concentrations increased by age (p < 0.001, r = 0.646) while no effect was observed in means of cadmium. Na+/K+ ATPase activities in the erythrocyte membranes were not affected.

In conclusion although there was no difference between the Na+/K+ ATPase activity in means of age, there was an environmental pollution and may be it was due to the industrial plant.


blood plasma cadmium cement Na+/K+ATPase nickel 


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  1. Bayhan, Y. K. and Özbay, O.: 1992, ‘Çimento toz emisyonlarının bazı bitkilerin büyüme ve metabolik olaylarına etkileri üzerine incelemeler,’, in: Z. Ayvaz (ed.), Çevre Kirliliği ve Kontrolü, 1. Uluslar arası çevre koruma sempozyumu bildiri kitabı, Ege University, İzmir, Turkey, pp. 302–313.Google Scholar
  2. Cavallo, D., Ursini, C. L., Setini, A., Chianese, C., Piegari, P., Perniconi, B. and Iavicoli, S.: 2003, ‘Evaluation of oxidative damage and inhibition of DNA repair in an in vitro study of nickel exposure,’, Toxicol. Vitro. 17, 603–607.Google Scholar
  3. Dodge, J. T., Mitchell, C. and Hanahan, D. J.: 1963, ‘The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes,’, Arch. Biochem. Biophys. 100, 119–130.Google Scholar
  4. Golik, A., Weissgarten, J., Evans, S., Cohen, N., Averbukh, Z., Zaidenstein, R., Cotariu, D. and Modia, D.: 1996, ‘Erythrocyte Na+, K+ and Ca2 +, Mg2 +-ATPase activities in hypertensives on angiotensin-converting enzyme inhibitors,’, Clin. Biochem. 29(3), 249–254.Google Scholar
  5. Gündüz, T.: 1993, Kalitatif Analiz Laboratuvar Kitabı, Bilge Yayıncılık, Ankara.Google Scholar
  6. Işıklı, B., Demir, T. A., Ürer, S. M., Berber, A., Akar, T. and Kalyoncu, C.: 2003, ‘Effects of chromium exposure from a cement factory,’, Environ. Res. 91, 113–118.Google Scholar
  7. Kasprzak, K. S., Sunderman, Jr., F. W. and Salnikow, K.: 2003, ‘Nickel carcinogenesis,’, Mutat. Res. 533, 67–97.Google Scholar
  8. Kızıler, A. R. and Barutçu, U. B.: 1997, ‘Canlıların çinko düzeylerine çevresel faktörlerin etkilerinin incelenmesi,’, in: Ulusal Çinko Kongresi (Tarım ve sağık), Anadolu Tarımsal Araştırma Enstitüsü, Eskişehir, Turkey.Google Scholar
  9. Linden, J. V., Hopfer, S. M., Grossling, H. R. and Sunderman, F. W.: 1985, ‘Blood nickel concentrations in patients wih stainless-steel hip prostheses,’, Ann. Clin. Lab. Sci. 15(6), 459–464.Google Scholar
  10. Manahan, S. E.: 1994, Environmental Chemistry, CRC Press, Florida, pp. 303–305.Google Scholar
  11. Martinsen, I. and Thomassen, Y.: 1983, ‘The Application of Atomic Spectroscopy in the Control of Industrial Exposure to Toxic Elements,’, in: S. S. Brown and J. Savory (eds), Proceedings of the Second International Conference on Clinical Toxicology and Clinical Chemistry of Metals, 19–22 July 1983, Montreal, Canada.Google Scholar
  12. Matilla, L. and Kilpelainen, M.: 2001, ‘Prevalence of nickel allergy among finnish university students in 1995,’, Contact Dermatits 44(4), 218–223.Google Scholar
  13. Milne, D. B.: 1994, ‘Trace Elements,’, in: C. A. Burtis and E. R. Ashwood (eds), Tıetz Texbook of Clinical Chemistry, 2nd ed., W.B. Saunders Company, Philadelphia, Pennsylvania, pp. 1347–1348.Google Scholar
  14. Que Hee, S. S. and Boyle, J. R.: 1988, ‘Simultaneous multielemental analyses of some environmental and biological samples by inductively coupled plasma atomic emission spectrometry,’, Anal. Chem. 60, 1033–1042.Google Scholar
  15. Rondia, D.: 1978, ‘Sources, Modes and Levels of Human Exposure to Chromium and Nickel,’, in: E. D. Ferrante (ed.), Proceedings of the Research Seminar on Trace Metals: Exposure and Health Effects, 10–13 July 1978, University of Surrey, Guildford, UK, Pergamon Press, pp. 117–134.Google Scholar
  16. Schoner, W., von Ilberg, C., Kramer, R. and Schubert, W.: 1967, ‘On the mechanism of Na+, K+-stimulated hydrolysis of adenosine triphosphate. Purification and properties of a Na+- and K+ activated ATPase from ox brain,’, Eur. J. Biochem. 1, 334–343.Google Scholar
  17. Silverman, L. M. and Christenson, R. H.: 1994, ‘Amino Acids and Proteins,’, in: C. A. Burtis and E. R. Ashwood (eds), Tıetz Texbook of Clinical Chemistry, 2nd ed., W.B. Saunders Company, Philadelphia, Pennsylvania, pp. 696–698.Google Scholar
  18. Spiegel, H.: 2002, ‘Trace element accumulation in selected bioindicators exposed to emissions along the industrial facilities of Danube Lowland,’, Turk. J. Chem. 26, 815–823.Google Scholar
  19. Waalkes, M. P.: 2003, ‘Cadmium carcinogenesis,’, Mutat. Res. 553, 107–120.Google Scholar
  20. Woodford, J., Gossman, R. and Gossman, D.: 1992, ‘Metal Precompliance for Cement Kilns,’, in: Proceedings of the Presentation at the AWMA International Speciality Conference on Waste Combustion in Boilers and Industrial Furnaces, March 1992.Google Scholar
  21. World Health Organization (WHO): 1991, Nickel Environmental Health, Criteria no: 108, Geneva.Google Scholar
  22. World Health Organization (WHO): 1992, Cadmium-Environmental Aspects Environmental Health, Criteria no: 135, Geneva.Google Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Temİr Alİ Demİr
    • 1
  • Tamer Akar
    • 1
  • Fahrettİn AkyÜz
    • 2
  • Burhanettİn IŞikli
    • 3
  • GÜngÖr Kanbak
    • 2
  1. 1.Department of Chemistry, Faculty of Arts and SciencesOsmangazi UniversityEskişehirTurkey
  2. 2.Department of Biochemistry, Faculty of MedicineOsmangazi UniversityEskişehirTurkey
  3. 3.Department of Public Health, Faculty of MedicineOsmangazi UniversityEskişehirTurkey

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