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A survey on the infection of Onion yellow dwarf virus and Iris yellow spot tospovirus in seed and bulb productions systems of onion in Calabria, Italy

  • Ariana Manglli
  • Laura Tomassoli
  • Antonio Tiberini
  • Giovanni E. Agosteo
  • Anna Fontana
  • Hanu R. Pappu
  • Giuliana AlbaneseEmail author
Article
  • 35 Downloads

Abstract

A survey on the incidence of onion yellow dwarf virus (OYDV) and iris yellow spot virus (IYSV) was carried out over three production cycles of onion ‘Rossa di Tropea’ in Calabria, Italy. OYDV was found to be the prevalent virus. ‘Rossa di Tropea’ seedbeds adjacent to OYDV-infected green onion field had seedlings with 1.76% infection rate determining 36.2% and 98.67% infected plants in the bulbs and in the subsequent seed harvesting times, respectively. When seedbeds were at least one km away from other onion crops seedlings and bulb cultivation had the infection rate close to zero. OYDV was detected in whole plants except the roots and outer desiccated bulb skins. Seed transmission was not detected in ‘Rossa di Tropea’. Early OYDV infection significantly reduced the number and weight of seeds/inflorescence compared to late season infection, while the weight of 100 seeds was not different in the two early and late OYDV infected plants. IYSV was never found in seedbeds. It was always detected first in seed crops (April) than in bulb crops (June), and the final infection rate was higher in seed (2.67%–3.33%) than in bulb crops (0%–0.87%), suggesting there was an internal source of viral inoculum in the field. IYSV was detected in 3/123 apex bulbs randomly collected from stored bulbs and in 12/12 apex fresh bulbs collected at harvest time from infected plants, suggesting the role of bulbs as IYSV inoculum source. On the contrary, randomly collected bulbs (N = 109) from warehouse and bulbs of infected plants (N = 22), transplanted after storage, did not result in IYSV-infected plants.

Keywords

Onion cv Rossa di Tropea OYDV IYSV Italy Virus detection Virus transmission 

Notes

Acknowledgements

This study was partially supported by Regione Calabria, Italy, Accordo di Programma Quadro (APQ) Ricerca Scientifica e Innovazione Tecnologica, Azione 3, “Innovazione di Filiera per la Valorizzazione della Cipolla Rossa di Tropea IGP”, and the Italian Ministry of Education, University and Research in the ‘Scientific Independence of young Researcher’ (SIR) programme, project “Study on Interaction between Onion yellow dwarf virus and nutraceutical compounds of ‘Rossa di Tropea’ onion” (SI.ORTO).

Authors thank Prof. Rita Marullo (Mediterranean University of Reggio Calabria, Italy) for identifying Thrips tabaci and Dr. Salvatore Vitale (Research Centre for Plant Protection and Certification, Rome, Italy) for identifying Alternaria spp. and Peronospora destructor in onion samples during this survey.

Compliance with ethical standards

Ethical statement

This research did not involve any animal and/or human participant. The authors declare that they have no conflict of interest.

Supplementary material

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ESM 1 (DOCX 79 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2020

Authors and Affiliations

  • Ariana Manglli
    • 1
    • 2
  • Laura Tomassoli
    • 2
  • Antonio Tiberini
    • 1
  • Giovanni E. Agosteo
    • 1
  • Anna Fontana
    • 1
  • Hanu R. Pappu
    • 3
  • Giuliana Albanese
    • 1
    Email author
  1. 1.Department of AGRARIAUniversità degli Studi Mediterranea di Reggio CalabriaReggio CalabriaItaly
  2. 2.Consiglio per la Ricerca in agricoltura e l’analisi dell’Economia Agraria, Research Centre for Plant Protection and CertificationRomeItaly
  3. 3.Department of Plant PathologyWashington State UniversityPullmanUSA

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