Morphological characteristics and genetic diversity of Colletotrichum horii infecting persimmon tree in China

  • Quan-en Deng
  • Xiang-yang Ding
  • Jian-an LiEmail author
  • Lin-kai Cui
  • Jian-qiang Xu


Anthracnose is the most devastating disease affecting persimmon (Diospyros kaki Thunb.) cultivars in China, and is caused by the pathogen recently identified as Colletotrichum horii. In this study, the symptoms of anthracnose in persimmon fruits and trees in China, and the morphological characteristics of C. horii, were observed and recorded. Thirty simple sequence repeat (SSR) markers for C. horii were developed from the whole genome of the closely related species C. gloeosporioides, which is available from the GenBank database. After screening 30 combinations of primer pairs, six SSR markers were selected to amplify the genomic DNA of 23 isolates, to assess the degree of polymorphism and the reproducibility of the SSR markers. A total of 164 discernible bands were obtained when visualizing the amplicons by electrophoresis, 156 of which showed polymorphism. A dendrogram was constructed with FreeTree software (ver. and the similarity coefficients ranged from 0.58–0.98. The result indicated there was genetic diversity in the C. horii population in China. There was an obvious correlation between the cluster groups and the sites from which the samples were isolated. The results of this study will facilitate a more detailed understanding of the population structure of C. horii in China.


Colletotrichum horii Morphological characteristics Simple sequence repeat Genetic diversity 



This research was supported by the Special Fund for Agroscientific Research in the Public Interest (201203047) and the Scientific Innovation Fund for Graduate of Central South University of Forestry and Technology (20181003).

The English in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, please see:

Compliance with ethical standards

Ethical statement

The authors declare that they have no conflict of interest. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.


  1. Agarwal, M., Shrivastava, N., & Padh, H. (2008). Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Reports, 4, 617–631. Scholar
  2. Cai, L., Hyde, K., Taylor, P., Weir, B., Waller, J., Abang, M., Zhang, J.-Z., Yang, Y.-L., Phoulivong, S., Liu, Z.-Y., Prihastuti, H., Shivas, R., McKenzie, E., & Johnston, P. (2009). A polyphasic approach for studying Colletotrichum. Fungal Diversity, 2, 183–204.Google Scholar
  3. Chung, K.-R., Shilts, T., Ertürk, Ü., Timmer, L.-W., & Ueng, P.-P. (2003). Indole derivatives produced by the fungus Colletotrichum acutatum causing lime anthracnose and postbloom fruit drop of citrus. FEMS Microbiology Letters, 226(1), 23–30. Scholar
  4. Damm, U., Baroncelli, R., Cai, L., Kubo, Y., O’Connell, R., Weir, B., Yoshino, K., & Cannon, P. (2010). Colletotrichum: Species, ecology and interactions. IMA Fungus, 2, 161–165. Scholar
  5. Dobrowolski, M., Tommerup, I., Blakeman, H., & O’Brien, P. (2002). Non-Mendelian inheritance revealed in a genetic analysis of sexual progeny of Phytophthora cinnamomi with microsatellite markers. Fungal Genetics and Biology, 35, 197–212. Scholar
  6. Fajolu, O., Wadl, P., Vu, A., Gwinn, K., Scheffler, B., Trigiano, R., & Ownley, B. (2013). Development and characterization of simple sequence repeats for Bipolaris sorokiniana and cross transferability to related species. Mycologia, 105, 1164. Scholar
  7. Freeman, S., Katan, T., & Shabi, E. (1998). Characterization of Colletotrichum species responsible for anthracnose diseases of various fruits. Plant Disease, 82, 596–605.CrossRefGoogle Scholar
  8. Garzón, C., Geiser, D., & Moorman, G. (2005). Diagnosis and population analysis of Pythium species using AFLP fingerprinting. Plant Disease, 89, 81–89. Scholar
  9. Guan, C.-F., Chen, W.-X., Mo, R.-L., Du, X.-Y., Zhang, Q.-L., & Luo, Z.-R. (2016). Isolation and characterization of DkPK genes associated with natural Deastringency in C-PCNA persimmon. Frontiers in Plant Science, 112, 1–11. Scholar
  10. Hemelrijck, W., Debode, J., Heungens, K., Maes, M., & Creemers, P. (2010). Phenotypic and genetic characterization of Colletotrichum isolates from Belgian strawberry fields. Plant Pathology, 59, 853–861. Scholar
  11. Hori, S. (1910). Kaki no Shinbyogai Tansobyo. Engei no Tomo, 6, 21–24.Google Scholar
  12. Ito, S. (1911). Gloeosporiose of the Japanese persimmon. The Botanical Magazine (Tokyo), 25, 197–202.CrossRefGoogle Scholar
  13. Ivors, K., Garbelotto, M., Vries, I., Ruyterspira, C., Hekkert, B., Rosenzweig, N., & Bonants, P. (2010). Microsatellite markers identify three lineages of Phytophthora ramorum in US nurseries, yet single lineages in US forest and European nursery populations. Molecular Ecology, 6, 1493–1505. Scholar
  14. Jyufuku, S., Furuya, N., Goto, T., Tsuchiya, K., & Yoshimura, A. (2009). Pathogenic and genetic diversity in Asian strains of Xanthomonas oryzae pv. oryzae. Journal of the Faculty of Agriculture Kyushu University, 54(1), 19–23. Scholar
  15. Kim, K., Yoon, J., Park, H., Park, E., & Kim, Y. (2004). Structural modifications and programmed cell death of chili pepper fruit related to resistance responses to Colletotrichum gloeosporioides infection. Phytopathology, 94, 1295–1304. Scholar
  16. Kono, A., Nakaune, R., Yamada, M., Nakano, M., Mitani, N., & Ueno, T. (2009). Effect of culture conditions on conidia formation by Elsinoë ampelina, the causal organism of grapevine anthracnose. Plant Disease, 93(5), 481–484. Scholar
  17. Kwon, J.-H., & Park, C.-S. (2004). Dissemination of conidiospores of Colletotrichum gloeosporiodes, the anthracnose of persimmon and the disease development. Research Plant Disease, 10, 272–278. Scholar
  18. Lee, S.-H., & Moorman, G. (2008). Identification and characterization of simple sequence repeat markers for Pythium aphanidermatum, P. cryptoirregulare, and P. irregulare and the potential use in Pythium population genetics. Current Genetics, 53, 81–93. Scholar
  19. Liu, K.-Q., & Mu, H.-F. (1988). Studies on persimmon anthracnose (Gloeosporium kaki hori). Journal of Shandong Agricultural University, 19, 69–71.Google Scholar
  20. Macdonald, B. (2004). Population genetics of plant pathogens. Plant Health Instructor.Google Scholar
  21. Maffei, L. (1921). Una malattia delle foglie del “Kaki” dovuta al Colletotrichum kaki n. sp. Rivista di Patologia Vegetale, 11, 116–118.Google Scholar
  22. Mahuku, G., & Riascus, J. (2004). Virulence and molecular diversity within Colletotrichum lindemuthianum isolates from Andean and Mesoamerican bean varieties and regions. European Journal of Plant Pathology, 110, 253–263. Scholar
  23. Matsumoto, C., Kageyama, K., Suga, H., & Hyakumachi, M. (2000). Intraspecific DNA polymorphisms of Pythium irregulare. Mycological Research, 104, 1333–1341. Scholar
  24. Middleton, J. (1943). The taxonomy, host range and geographic distribution of the genus Pythium. Memoirs of the Torrey Botanical Club, 20, 1–171.Google Scholar
  25. Min, T., Yin, X.-R., Shi, Y.-N., Luo, Z.-R., Yao, Y.-C., Donald, G., Ian, F., & Chen, K.-S. (2012). Ethylene-responsive transcription factors interact with promoters of ADH and PDC involved in persimmon (Diospyros kaki) fruit de-astringency. Journal of Experimental Botany, 18, 6393–6405. Scholar
  26. Mo, R.-L., Yang, S.-C., Huang, Y.-M., Chen, W.-X., Zhang, Q.-L., & Luo, Z.-R. (2016). ADH and PDC genes involved in tannins coagulation leading to natural de-astringency in Chinese pollination constant and non-astringency persimmon (Diospyros kaki Thunb.). Tree Genetics & Genomes, 2, 1–11. Scholar
  27. Nei, M., & Li, W. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America, 10, 5269–5273.CrossRefGoogle Scholar
  28. Peres, N.-A., Mackenzie, S.-J., Peever, T.-L., & Timmer, L.-W. (2008). Postbloom fruit drop of citrus and key lime anthracnose are caused by distinct phylogenetic lineages of Colletotrichum acutatum. Phytopathology, 98(3), 345–352. Scholar
  29. Sawada, K. (1933). Report of survey on fungi in Taiwan. Report of Agriculture Ministry of Taiwan Center Institute, 61, 1–99.Google Scholar
  30. Shi, X. Q., Li, B. Q., Qin, G. Z., & Tian, S. P. (2011). Antifungal activity and possible mode of action of borate against Colletotrichum gloeosporioides on mango. Plant Disease, 95(1), 63–69. Scholar
  31. Tautz, D., & Renz, M. (1984). Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Research, 10, 4127–4138.CrossRefGoogle Scholar
  32. Weir, B., & Johnston, P. (2010). Characterisation and neotypification of Gloeosporium kaki Hori as Colletotrichum horii nom. nov. Mycotaxon, 111, 209–219. Scholar
  33. Wharton, P., & Diéguez-Uribeondo, J. (2004). The biology of Colletotrichum acutatum. Anales del Jardín Botánico de Madrid, 61, 3–22. Scholar
  34. Xie, L., Zhang, J.-Z., Cai, L., & Hyde, K. (2010). Biology of Colletotrichum horii, the causal agent of persimmon anthracnose. Mycology, 1(4), 242–253.CrossRefGoogle Scholar
  35. Yamada, M. (2004). Persimmon genetic resources and breeding in Japan. Acta Horticulture, 685, 55–64. Scholar
  36. Yonemori, K., Honsho, C., Kanzaki, S., Ino, H., Ikegami, A., Kitajima, A., Sugiura, A., & Parfitt, D. (2008). Sequence analyses of the ITS regions and the mat K gene for determining phylogenetic relationships of Diospyros kaki (persimmon) with other wild Diospyros (Ebenaceae) species. Tree Genetics & Genomes, 4, 149–158. Scholar
  37. Zhimo, V. Y., Dilip, D., Sten, J., Ravat, V. K., Bhutia, D. D., Panja, B., et al. (2017). Antagonistic yeasts for biocontrol of the banana postharvest anthracnose pathogen Colletotrichum musae. Journal of Phytopathology, 165(1), 35–43. Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  • Quan-en Deng
    • 1
  • Xiang-yang Ding
    • 2
  • Jian-an Li
    • 1
    Email author
  • Lin-kai Cui
    • 3
  • Jian-qiang Xu
    • 3
  1. 1.Key Laboratory of Non-Wood Forest Product of State Forestry Administration, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education, College of ForestryCentral South University of Forestry and TechnologyChangshaChina
  2. 2.Henan Academy of ForestryZhengzhouChina
  3. 3.Henan University of Science and TechnologyLuoyangChina

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